Results 31 to 40 of about 743,554 (284)

Direct oligonucleotide sequencing with nanopores [PDF]

Open Research Europe, 2020
Third-generation DNA sequencing has enabled sequencing of long, unamplified DNA fragments with minimal steps. Direct sequencing of ssDNA or RNA gives valuable insights like base-level modifications, phosphoramidite synthesis yield estimates and strand quality analysis, without the need to add the complimentary strand.
Chalapati, Sachin, Crosbie, Conor A, Limbachiya, Dixita, Pinnamaneni, Nimesh   +3 more
openaire   +4 more sources

Metagenomic identification of severe pneumonia pathogens in mechanically-ventilated patients: a feasibility and clinical validity study

Respiratory Research, 2019
Background Metagenomic sequencing of respiratory microbial communities for pathogen identification in pneumonia may help overcome the limitations of culture-based methods. We examined the feasibility and clinical validity of rapid-turnaround metagenomics
Libing Yang, Ghady Haidar, Haris Zia, Rachel Nettles, Shulin Qin, Xiaohong Wang, Faraaz Shah, Sarah F. Rapport, Themoula Charalampous, Barbara Methé, Adam Fitch, Alison Morris, Bryan J. McVerry, Justin O’Grady, Georgios D. Kitsios   +14 more
doaj   +1 more source

Modification mapping by nanopore sequencing

Frontiers in Genetics, 2022
Next generation sequencing (NGS) has provided biologists with an unprecedented view into biological processes and their regulation over the past 2 decades, fueling a wave of development of high throughput methods based on short read DNA and RNA sequencing. For nucleic acid modifications, NGS has been coupled with immunoprecipitation, chemical treatment,
Laura K. White, Jay R. Hesselberth
openaire   +3 more sources

Nanopore sequencing in non-human forensic genetics [PDF]

, 2021
:The past decade has seen a rapid expansion of non-human forensic genetics coinciding with the development of 2nd and 3rd generation DNA sequencing technologies.
Vasiljevic, Nina, Ogden, Rob, Ogden, Rob; https://orcid.org/, Prost, Stefan, Vasiljevic, Nina; https://orcid.org/   +4 more
core   +1 more source

Assessing FOXA1-driven mRNA processing in prostate cancer with Nanopore long-read sequencing

, 2022
Despite advances in the diagnosis and treatment of early stage of prostate cancer (PC), there are few therapeutic options for end-stage metastatic castration-resistant PC.
Matteo Cereda
core   +2 more sources

From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy

Genome Biology, 2018
Nanopore sequencing is a rapidly maturing technology delivering long reads in real time on a portable instrument at low cost. Not surprisingly, the community has rapidly taken up this new way of sequencing and has used it successfully for a variety of ...
Franka J. Rang, Wigard P. Kloosterman, Jeroen de Ridder   +2 more
doaj   +1 more source

A world of opportunities with nanopore sequencing [PDF]

Journal of Experimental Botany, 2017
Oxford Nanopore Technologies' MinION sequencer was launched in pre-release form in 2014 and represents an exciting new sequencing paradigm. The device offers multi-kilobase reads and a streamed mode of operation that allows processing of reads as they are generated.
Richard M Leggett, Matthew D Clark
openaire   +2 more sources

Application of nanopore adaptive sequencing in pathogen detection of a patient with Chlamydia psittaci infection

Frontiers in Cellular and Infection Microbiology, 2023
IntroductionNanopore sequencing has been widely used in clinical metagenomic sequencing for pathogen detection with high portability and real-time sequencing.
Yanfeng Lin, Yanfeng Lin, Yan Dai, Shuang Zhang, Shuang Zhang, Hao Guo, Lang Yang, Jinhui Li, Kaiying Wang, Ming Ni, Ming Ni, Zongqian Hu, Zongqian Hu, Leili Jia, Huiying Liu, Peng Li, Hongbin Song, Hongbin Song   +17 more
doaj   +1 more source

De novo whole-genome assembly of a wild type yeast isolate using nanopore sequencing [version 2; referees: 1 approved, 3 approved with reservations]

F1000Research, 2018
Background: The introduction of the MinION sequencing device by Oxford Nanopore Technologies may greatly accelerate whole genome sequencing. Nanopore sequence data offers great potential for de novo assembly of complex genomes without using other ...
Michael Liem, Hans J. Jansen, Ron P. Dirks, Christiaan V. Henkel, G. Paul H. van Heusden, Richard J.L.F. Lemmers, Trifa Omer, Shuai Shao, Peter J. Punt, Herman P. Spaink   +9 more
doaj   +1 more source

NanoSTR: A method for detection of target short tandem repeats based on nanopore sequencing data

Frontiers in Molecular Biosciences, 2023
Short tandem repeats (STRs) are widely present in the human genome. Studies have confirmed that STRs are associated with more than 30 diseases, and they have also been used in forensic identification and paternity testing.
Jidong Lang, Zhihua Xu, Yue Wang, Jiguo Sun, Zhi Yang   +4 more
doaj   +1 more source