Results 51 to 60 of about 743,554 (284)

Accurate Detection of HPV Integration Sites in Cervical Cancer Samples Using the Nanopore MinION Sequencer Without Error Correction

Frontiers in Genetics, 2020
During the carcinogenesis of cervical cancer, the DNA of human papillomavirus (HPV) is frequently integrated into the human genome, which might be a biomarker for the early diagnosis of cervical cancer.
Wenjuan Yang, Ying Liu, Ruyi Dong, Jia Liu, Jidong Lang, Jialiang Yang, Weiwei Wang, Jingjing Li, Bo Meng, Geng Tian, Geng Tian   +10 more
doaj   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Comparative analysis of hybrid-SNP microarray and nanopore sequencing for detection of large-sized copy number variants in the human genome

Molecular Cytogenetics
Background Nanopore sequencing is a technology that holds great promise for identifying all types of human genome variations, particularly structural variations.
Catarina Silva, José Ferrão, Bárbara Marques, Sónia Pedro, Hildeberto Correia, Ana Valente, António Sebastião Rodrigues, Luís Vieira   +7 more
doaj   +1 more source

DNA Sequencing by Hexagonal Boron Nitride Nanopore: A Computational Study

Nanomaterials, 2016
The single molecule detection associated with DNA sequencing has motivated intensive efforts to identify single DNA bases. However, little research has been reported utilizing single-layer hexagonal boron nitride (hBN) for DNA sequencing.
Liuyang Zhang, Xianqiao Wang
doaj   +1 more source

Nanopore sequencing data of transconjugants

, 2023
Nanopore sequencing data of transconjugants.
yan li (6186836)
core   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Nanopore sequencing for smear-negative pulmonary tuberculosis—a multicentre prospective study in China

Annals of Clinical Microbiology and Antimicrobials
Purpose In this prospective study, the diagnosis accuracy of nanopore sequencing-based Mycobacterium tuberculosis (MTB) detection was determined through examining bronchoalveolar lavage fluid (BALF) samples from pulmonary tuberculosis (PTB) -suspected ...
Xiaojing Yan, Guoli Yang, Yunfei Wang, Yuqing Wang, Jie Cheng, Peisong Xu, Xiaoli Qiu, Lei Su, Lina Liu, Ruixue Geng, Yingxia You, Hui Liu, Naihui Chu, Li Ma, Wenjuan Nie   +14 more
doaj   +1 more source

Evaluation of multiplex nanopore sequencing for Salmonella serotype prediction and antimicrobial resistance gene and virulence gene detection

Frontiers in Microbiology, 2023
In a previous study, Multiplex-nanopore-sequencing based whole genome sequencing (WGS) allowed for accurate in silico serotype prediction of Salmonella within one day for five multiplexed isolates, using both SISTR and SeqSero2.
Xingwen Wu, Hao Luo, Chongtao Ge, Feng Xu, Xiangyu Deng, Martin Wiedmann, Robert C. Baker, Abigail E. Stevenson, Guangtao Zhang, Silin Tang   +9 more
doaj   +1 more source

Expanding applications for nanopore sequencing [PDF]

Nature Reviews Genetics, 2019
Two new studies report advances for nanopore-based long-read sequencing for characterizing complex human transcriptomes and for analysing human repetitive DNA regions.
openaire   +2 more sources