Results 41 to 50 of about 743,554 (284)
An educational guide for nanopore sequencing in the classroom [PDF]
PLOS Computational Biology, 2020 The last decade has witnessed a remarkable increase in our ability to measure genetic information. Advancements of sequencing technologies are challenging the existing methods of data storage and analysis. While methods to cope with the data deluge are progressing, many biologists have lagged behind due to the fast pace of computational advancements ...
Alex Salazar +11 more
openaire +6 more sources
MethPhaser: methylation-based long-read haplotype phasing of human genomes
Nature CommunicationsThe assignment of variants across haplotypes, phasing, is crucial for predicting the consequences, interaction, and inheritance of mutations and is a key step in improving our understanding of phenotype and disease.
Yilei Fu +6 more
doaj +1 more source
Application of Nanopore Sequencing for High Throughput Genotyping in Horses
Animals, 2023 Nanopore sequencing is a third-generation biopolymer sequencing technique that relies on monitoring the changes in an electrical current that occur as nucleic acids are passed through a protein nanopore.
Artur Gurgul +6 more
doaj +1 more source
, 2019 nanopore_03262018.fastq.gz Description: DNA sample of individual NS12911 was sequenced by Oxford Nanopore (ONT) MinION using flowcell FLO-MIN106D. The library was constructed using ONT 1D Genomic DNA by ligation protocol.
core +1 more source
Nanopore-based technologies beyond DNA sequencing [PDF]
, 2022 Inspired by the biological processes of molecular recognition and transportation across membranes, nanopore techniques have evolved in recent decades as ultrasensitive analytical tools for individual molecules.
Fragasso, Alessio +9 more
core +2 more sources
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Molecular Oncology, EarlyView.Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley +1 more source
BMC GenomicsBackground The endoparasitic rhizocephalan Parasacculina sinensis has a radically simplified morphology and primarily infests decapods crustaceans. Rhizocephalan barnacles usually absorb nutrients from the host through a complex rootlet system (the ...
Wenjie Li +4 more
doaj +1 more source
High-Quality Genome-Scale Models From Error-Prone, Long-Read Assemblies
Frontiers in Microbiology, 2020 Advances in nanopore-based sequencing techniques have enabled rapid characterization of genomes and transcriptomes. An emerging application of this sequencing technology is point-of-care characterization of pathogenic bacteria.
Jared T. Broddrick +5 more
doaj +1 more source
Molecular Oncology, EarlyView.This study identifies somatostatin receptor 4 (Sstr4) as a critical tumor suppressor against skin and head/neck cancers (HNSCC, cSCC, and BCC). The loss of Sstr4 removes a check on cell growth, causing hyperactivation of the MAPK‐ERK signaling pathway (↑).
Ali Taqvi +6 more
wiley +1 more source