Results 21 to 30 of about 47,109 (297)

Whole-Genome Sequencing of Human Enteroviruses from Clinical Samples by Nanopore Direct RNA Sequencing. [PDF]

, 2020
Enteroviruses are small RNA viruses that affect millions of people each year by causing an important burden of disease with a broad spectrum of symptoms.
Carole Grädel, Baumann, Christian, Terrazos Miani, Miguel A., Grädel, Carole, Miguel A. Terrazos Miani, Barbani, Maria Teresa, Stephen L. Leib, Maria Teresa Barbani, Ramette, Alban, Christian Baumann, Leib, Stephen L., Alban Ramette, Neuenschwander, Stefan, Suter-Riniker, Franziska, Stefan Neuenschwander, Franziska Suter-Riniker   +15 more
core   +1 more source

Rolling circle reverse transcription enables high fidelity nanopore sequencing of small RNA.

PLoS ONE, 2022
Small RNAs (sRNAs) are an important group of non-coding RNAs that have great potential as diagnostic and prognostic biomarkers for treatment of a wide variety of diseases. The portability and affordability of nanopore sequencing technology makes it ideal
Sean Maguire, Shengxi Guan
doaj   +2 more sources

Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome

Genome Medicine, 2019
Background Intrachromosomal triplications (TRP) can contribute to disease etiology via gene dosage effects, gene disruption, position effects, or fusion gene formation.
Claudia M. B. Carvalho, Zeynep Coban-Akdemir, Hadia Hijazi, Bo Yuan, Matthew Pendleton, Eoghan Harrington, John Beaulaurier, Sissel Juul, Daniel J. Turner, Rupa S. Kanchi, Shalini N. Jhangiani, Donna M. Muzny, Richard A. Gibbs, Baylor-Hopkins Center for Mendelian Genomics, Pawel Stankiewicz, John W. Belmont, Chad A. Shaw, Sau Wai Cheung, Neil A. Hanchard, V. Reid Sutton, Patricia I. Bader, James R. Lupski   +21 more
doaj   +1 more source

Nanopore-based metagenomic sequencing for the rapid and precise detection of pathogens among immunocompromised cancer patients with suspected infections

Frontiers in Cellular and Infection Microbiology, 2022
Cancer patients are at high risk of infections and infection-related mortality; thereby, prompt diagnosis and precise anti-infectives treatment are critical.
Qingmei Deng, Qingmei Deng, Qingmei Deng, Yongqing Cao, Xiaofeng Wan, Xiaofeng Wan, Bin Wang, Aimin Sun, Aimin Sun, Huanzhong Wang, Huanzhong Wang, Yunfei Wang, Hongzhi Wang, Hongzhi Wang, Hongzhi Wang, Hongcang Gu, Hongcang Gu, Hongcang Gu   +17 more
doaj   +1 more source

DNA methylation-calling tools for Oxford Nanopore sequencing: a survey and human epigenome-wide evaluation

Genome Biology, 2021
Background Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads.
Yang Liu, Wojciech Rosikiewicz, Ziwei Pan, Nathaniel Jillette, Ping Wang, Aziz Taghbalout, Jonathan Foox, Christopher Mason, Martin Carroll, Albert Cheng, Sheng Li   +10 more
doaj   +1 more source

Three decades of nanopore sequencing [PDF]

Nature Biotechnology, 2016
A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule through that pore. With the development of enzyme-based methods that ratchet polynucleotides through the nanopore, nucleobase-by-nucleobase, measurements of changes in the current ...
David, Deamer, Mark, Akeson, Daniel, Branton   +2 more
openaire   +2 more sources

Nanopore sequencing data of transconjugants [PDF]

, 2023
Nanopore sequencing data of transconjugants.
yan li (6186836)
core   +1 more source

Direct oligonucleotide sequencing with nanopores [PDF]

Open Research Europe, 2020
Third-generation DNA sequencing has enabled sequencing of long, unamplified DNA fragments with minimal steps. Direct sequencing of ssDNA or RNA gives valuable insights like base-level modifications, phosphoramidite synthesis yield estimates and strand quality analysis, without the need to add the complimentary strand.
Chalapati, Sachin, Crosbie, Conor A, Limbachiya, Dixita, Pinnamaneni, Nimesh   +3 more
openaire   +4 more sources

Evaluating nanopore sequencing data processing pipelines for structure variation identification, supplemental data [PDF]

, 2019
nanopore_03262018.fastq.gz Description: DNA sample of individual NS12911 was sequenced by Oxford Nanopore (ONT) MinION using flowcell FLO-MIN106D. The library was constructed using ONT 1D Genomic DNA by ligation protocol.

core   +1 more source

Simulation of Nanopore Sequencing Signals Based on BiGRU

Sensors, 2020
Oxford Nanopore sequencing is an important sequencing technology, which reads the nucleotide sequence by detecting the electrical current signal changes when DNA molecule is forced to pass through a biological nanopore.
Weigang Chen, Peng Zhang, Lifu Song, Jinsheng Yang, Changcai Han   +4 more
doaj   +1 more source