Results 21 to 30 of about 743,554 (284)

The potential and challenges of nanopore sequencing [PDF]

Nature Biotechnology, 2008
A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect ...
Branton, Daniel, Pershin, Yuriy V, Deamer, David W, Ling, Xinsheng Sean, Wiggin, Matthew, Lindsay, Stuart, Krstic, Predrag S, Huang, Xiaohua, Jovanovich, Stevan B, Butler, Thomas, Soni, Gautam V, Schloss, Jeffery A, Riehn, Robert, Hibbs, Andrew, Tabard-Cossa, Vincent, Wanunu, Meni, Bayley, Hagan, Marziali, Andre, Ramsey, J Michael, Mastrangelo, Carlos H, Meller, Amit, Benner, Steven A, Oliver, John S, Garaj, Slaven, Di Ventra, Massimiliano   +24 more
openaire   +4 more sources

Whole-Genome Sequencing of Human Enteroviruses from Clinical Samples by Nanopore Direct RNA Sequencing. [PDF]

, 2020
Enteroviruses are small RNA viruses that affect millions of people each year by causing an important burden of disease with a broad spectrum of symptoms.
Carole Grädel, Baumann, Christian, Terrazos Miani, Miguel A., Grädel, Carole, Miguel A. Terrazos Miani, Barbani, Maria Teresa, Stephen L. Leib, Maria Teresa Barbani, Ramette, Alban, Christian Baumann, Leib, Stephen L., Alban Ramette, Neuenschwander, Stefan, Suter-Riniker, Franziska, Stefan Neuenschwander, Franziska Suter-Riniker   +15 more
core   +2 more sources

Matched Oxford Nanopore Technologies and Bisulfite Sequencing of the GM24385 Cell Line

, 2022
One of the most widespread genomic modifications is 5-methylcytosine (5mC), which most frequently occurs at CpG dinucleotides. Compared to whole-genome bisulfite sequencing, the traditional method of 5mC detection, nanopore technology can offer many ...
Oxford Nanopore Technologies
core   +1 more source

Adaptation of Oxford Nanopore technology for hepatitis C whole genome sequencing and identification of within-host viral variants

BMC Genomics, 2021
Background Hepatitis C (HCV) and many other RNA viruses exist as rapidly mutating quasi-species populations in a single infected host. High throughput characterization of full genome, within-host variants is still not possible despite advances in next ...
Nasir Riaz, Preston Leung, Kirston Barton, Martin A. Smith, Shaun Carswell, Rowena Bull, Andrew R. Lloyd, Chaturaka Rodrigo   +7 more
doaj   +1 more source

Genotyping of familial Mediterranean fever gene (MEFV)—Single nucleotide polymorphism—Comparison of Nanopore with conventional Sanger sequencing

PLoS ONE, 2022
Background Through continuous innovation and improvement, Nanopore sequencing has become a powerful technology. Because of its fast processing time, low cost, and ability to generate long reads, this sequencing technique would be particularly suitable ...
Jonas Schmidt, Sandro Berghaus, Frithjof Blessing, Holger Herbeck, Josef Blessing, Peter Schierack, Stefan Rödiger, Dirk Roggenbuck, Folker Wenzel   +8 more
doaj   +2 more sources

Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome

Genome Medicine, 2019
Background Intrachromosomal triplications (TRP) can contribute to disease etiology via gene dosage effects, gene disruption, position effects, or fusion gene formation.
Claudia M. B. Carvalho, Zeynep Coban-Akdemir, Hadia Hijazi, Bo Yuan, Matthew Pendleton, Eoghan Harrington, John Beaulaurier, Sissel Juul, Daniel J. Turner, Rupa S. Kanchi, Shalini N. Jhangiani, Donna M. Muzny, Richard A. Gibbs, Baylor-Hopkins Center for Mendelian Genomics, Pawel Stankiewicz, John W. Belmont, Chad A. Shaw, Sau Wai Cheung, Neil A. Hanchard, V. Reid Sutton, Patricia I. Bader, James R. Lupski   +21 more
doaj   +1 more source

Rolling circle reverse transcription enables high fidelity nanopore sequencing of small RNA.

PLoS ONE, 2022
Small RNAs (sRNAs) are an important group of non-coding RNAs that have great potential as diagnostic and prognostic biomarkers for treatment of a wide variety of diseases. The portability and affordability of nanopore sequencing technology makes it ideal
Sean Maguire, Shengxi Guan
doaj   +2 more sources

Nanopore-based metagenomic sequencing for the rapid and precise detection of pathogens among immunocompromised cancer patients with suspected infections

Frontiers in Cellular and Infection Microbiology, 2022
Cancer patients are at high risk of infections and infection-related mortality; thereby, prompt diagnosis and precise anti-infectives treatment are critical.
Qingmei Deng, Qingmei Deng, Qingmei Deng, Yongqing Cao, Xiaofeng Wan, Xiaofeng Wan, Bin Wang, Aimin Sun, Aimin Sun, Huanzhong Wang, Huanzhong Wang, Yunfei Wang, Hongzhi Wang, Hongzhi Wang, Hongzhi Wang, Hongcang Gu, Hongcang Gu, Hongcang Gu   +17 more
doaj   +1 more source

DNA methylation-calling tools for Oxford Nanopore sequencing: a survey and human epigenome-wide evaluation

Genome Biology, 2021
Background Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads.
Yang Liu, Wojciech Rosikiewicz, Ziwei Pan, Nathaniel Jillette, Ping Wang, Aziz Taghbalout, Jonathan Foox, Christopher Mason, Martin Carroll, Albert Cheng, Sheng Li   +10 more
doaj   +1 more source

Simulation of Nanopore Sequencing Signals Based on BiGRU

Sensors, 2020
Oxford Nanopore sequencing is an important sequencing technology, which reads the nucleotide sequence by detecting the electrical current signal changes when DNA molecule is forced to pass through a biological nanopore.
Weigang Chen, Peng Zhang, Lifu Song, Jinsheng Yang, Changcai Han   +4 more
doaj   +1 more source