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Forensic SNP genotyping using nanopore MinION sequencing [PDF]
, 2017 One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using
Cornelis, Senne +4 more
core +2 more sources
Recent advances in nanopore sequencing [PDF]
ELECTROPHORESIS, 2012 The prospect of nanopores as a next‐generation sequencing platform has been a topic of growing interest and considerable government‐sponsored research for more than a decade. Oxford Nanopore Technologies recently announced the first commercial nanopore sequencing devices, to be made available by the end of 2012, while other companies (Life, Roche, and ...
Raj D, Maitra +2 more
openaire +2 more sources
Nanopore DNA sequencing with MspA [PDF]
Proceedings of the National Academy of Sciences, 2010 Nanopore sequencing has the potential to become a direct, fast, and inexpensive DNA sequencing technology. The simplest form of nanopore DNA sequencing utilizes the hypothesis that individual nucleotides of single-stranded DNA passing through a nanopore will uniquely modulate an ionic current flowing through the pore, allowing the record ...
Ian M, Derrington +6 more
openaire +2 more sources
Simulation of Nanopore Sequencing Signals Based on BiGRU
Sensors, 2020 Oxford Nanopore sequencing is an important sequencing technology, which reads the nucleotide sequence by detecting the electrical current signal changes when DNA molecule is forced to pass through a biological nanopore.
Weigang Chen +4 more
doaj +1 more source
Modification mapping by nanopore sequencing
Frontiers in Genetics, 2022 Next generation sequencing (NGS) has provided biologists with an unprecedented view into biological processes and their regulation over the past 2 decades, fueling a wave of development of high throughput methods based on short read DNA and RNA sequencing. For nucleic acid modifications, NGS has been coupled with immunoprecipitation, chemical treatment,
Laura K. White, Jay R. Hesselberth
openaire +3 more sources
Identification of single nucleotides in MoS2 nanopores [PDF]
, 2015 Ultrathin membranes have drawn much attention due to their unprecedented spatial resolution for DNA nanopore sequencing. However, the high translocation velocity (3000-50000 nt/ms) of DNA molecules moving across such membranes limits their usability.
Bulushev, Roman D. +6 more
core +2 more sources
Colloquium: Physical approaches to DNA sequencing and detection [PDF]
, 2008 With the continued improvement of sequencing technologies, the prospect of genome-based medicine is now at the forefront of scientific research. To realize this potential, however, a revolutionary sequencing method is needed for the cost-effective and ...
Di Ventra, Massimiliano, Zwolak, Michael
core +2 more sources
Respiratory Research, 2019 Background Metagenomic sequencing of respiratory microbial communities for pathogen identification in pneumonia may help overcome the limitations of culture-based methods. We examined the feasibility and clinical validity of rapid-turnaround metagenomics
Libing Yang +14 more
doaj +1 more source
Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing [PDF]
, 2017 Compared with conventional methods, single-molecule real-time (SMRT) DNA sequencing exhibits longer read lengths than conventional methods, less GC bias, and the ability to read DNA base modifications.
Henley, Robert Y. +4 more
core +1 more source
Graphene Nanopores for Protein Sequencing [PDF]
Biophysical Journal, 2016 An inexpensive, reliable method for protein sequencing is essential to unraveling the biological mechanisms governing cellular behavior and disease. Current protein sequencing methods suffer from limitations associated with the size of proteins that can be sequenced, the time, and the cost of the sequencing procedures. This study reports the results of
Wilson, James +3 more
openaire +3 more sources