Results 11 to 20 of about 938,732 (287)

Amplification-Free Nanopore Sequencing for Herpesvirus DNA Detection in Intraocular Fluids [PDF]

Ophthalmology Science
Purpose: To evaluate the feasibility of amplification-free nanopore sequencing for detecting herpesvirus DNA in intraocular fluid using multiplex polymerase chain reaction (mPCR)-characterized herpesvirus-positive and herpesvirus-negative samples. Design:
Sho Yokoyama, MD, Ai Fujita Sajiki, MD, PhD, Hiroaki Ushida, MD, PhD, Ayana Suzumura, MD, PhD, Kosuke Fujita, PhD, Mitsuki Kosaka, MD, PhD, Kazuhisa Yamada, MD, PhD, Koji M. Nishiguchi, MD, PhD   +7 more
doaj   +2 more sources

Application of Nanopore Sequencing for High Throughput Genotyping in Horses

Animals, 2023
Nanopore sequencing is a third-generation biopolymer sequencing technique that relies on monitoring the changes in an electrical current that occur as nucleic acids are passed through a protein nanopore.
Artur Gurgul, Igor Jasielczuk, Tomasz Szmatoła, Sebastian Sawicki, Ewelina Semik-Gurgul, Bogusława Długosz, Monika Bugno-Poniewierska   +6 more
doaj   +2 more sources

Nanopore Sequencing Technology: A Reliable Method for Pathogen Diagnosis in Elderly Patients with Community-Acquired Pneumonia [PDF]

Infection and Drug Resistance
Xiyue Zhang,1,* Jian Ye,2,* Limin Wang,1 Liuhai Zhang,1 Liusheng Wang,1 Hualiang Jin1 1Department of Respiratory Medicine, Affiliated Hangzhou First People’s Hospital, School of Medicine, Westlake University, Hangzhou, People’s Republic of China;
Zhang X, Ye J, Wang L, Zhang L, Wang L, Jin H   +5 more
doaj   +1 more source

Optimized Cas9-Enriched Nanopore Sequencing and Analysis Workflow for Clinical Diagnosis of Repeat Expansion Disorders. [PDF]

Adv Sci (Weinh)
An optimized Cas9‐enriched nanopore sequencing workflow, combined with STRiker, enables simultaneous analysis of disease‐associated STR loci from patient blood. The nCATS–STRiker workflow detects repeat expansions, de novo repeat motifs, interruption patterns, and methylation in a single assay, improving the genetic diagnosis of previously undiagnosed ...
Lee S, Jung C, Kim M, Kim N, Hwang GH, Lee ST, Chu K, Lee SK, Kim HJ, Chae JH, Bae S, Moon J.   +11 more
europepmc   +2 more sources

Nanopore-based technologies beyond DNA sequencing [PDF]

, 2022
Inspired by the biological processes of molecular recognition and transportation across membranes, nanopore techniques have evolved in recent decades as ultrasensitive analytical tools for individual molecules.
Fragasso, Alessio, Long, Yi-Tao, Maglia, Giovanni, Qing, Yujia, Meller, Amit, Hu, Zheng-Li, Bayley, Hagan, Ying, Yi-Lun, Zhang, Shengli, Dekker, Cees   +9 more
core   +2 more sources

Utility of nanopore sequencing for detecting pathogens in bronchoalveolar lavage fluid from pediatric patients with respiratory failure

Journal of Clinical Virology Plus, 2023
RNA viruses are the most frequent pathogens responsible for respiratory infections, particularly in pediatric patients. Next-generation sequencing, represented by Illumina sequencing, is one of the most comprehensive methods for identifying pathogens ...
Makoto Yamaguchi, Kazuhiro Horiba, Kazunori Haruta, Suguru Takeuchi, Takako Suzuki, Yuka Torii, Shinji Kawabe, Sho Wada, Takanari Ikeyama, Yoshinori Ito, Tomoo Ogi, Jun-ichi Kawada   +11 more
doaj   +1 more source

Rolling circle reverse transcription enables high fidelity nanopore sequencing of small RNA.

PLoS ONE, 2022
Small RNAs (sRNAs) are an important group of non-coding RNAs that have great potential as diagnostic and prognostic biomarkers for treatment of a wide variety of diseases. The portability and affordability of nanopore sequencing technology makes it ideal
Sean Maguire, Shengxi Guan
doaj   +2 more sources

The Power of Using Novel Nanopore Sequencing Technology for Diagnosis, Genomic and Pathological Studies of Covid-19 [PDF]

E3S Web of Conferences, 2021
The novel coronavirus disease 2019 (COVID‐19), originally identified in December 2019 Wuhan, China, has propagated to worldwide pandemic, causing many cases of death and morbidity.
Du Chenghao
doaj   +1 more source

Freshwater monitoring by nanopore sequencing [PDF]

eLife, 2020
ABSTRACT While traditional microbiological freshwater tests focus on the detection of specific bacterial indicator species, including pathogens, direct tracing of all aquatic DNA through metagenomics poses a profound alternative.
Urban, Lara, Holzer, Andre, Baronas, J Jotautas, Hall, Michael, Braeuninger-Weimer, Philipp, Scherm, Michael J, Kunz, Daniel J, Perera, Surangi N, Martin-Herranz, Daniel E, Tipper, Edward T, Salter, Susannah J, Stammnitz, Maximilian R   +11 more
openaire   +7 more sources

Matched Oxford Nanopore Technologies and Bisulfite Sequencing of the GM24385 Cell Line

, 2022
One of the most widespread genomic modifications is 5-methylcytosine (5mC), which most frequently occurs at CpG dinucleotides. Compared to whole-genome bisulfite sequencing, the traditional method of 5mC detection, nanopore technology can offer many ...
Oxford Nanopore Technologies
core   +1 more source