Results 11 to 20 of about 998,588 (389)

Nanopore sequencing technology and its applications

MedComm, 2023
Since the development of Sanger sequencing in 1977, sequencing technology has played a pivotal role in molecular biology research by enabling the interpretation of biological genetic codes.
Peijie Zheng, Chuntao Zhou, Yuemin Ding, Bin Liu, Liuyi Lu, Feng Zhu, Shiwei Duan   +6 more
doaj   +4 more sources

An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations

Micromachines, 2023
There has been significant progress made in the field of nanopore biosensor development and sequencing applications, which address previous limitations that restricted widespread nanopore use.
Morgan MacKenzie, Christos Argyropoulos
doaj   +2 more sources

DNA methylation-calling tools for Oxford Nanopore sequencing: a survey and human epigenome-wide evaluation

Genome Biology, 2021
Background Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads.
Yang Liu, Wojciech Rosikiewicz, Ziwei Pan, Nathaniel Jillette, Ping Wang, Aziz Taghbalout, Jonathan Foox, Christopher Mason, Martin Carroll, Albert Cheng, Sheng Li   +10 more
doaj   +2 more sources

The Evolution of Nanopore Sequencing [PDF]

Frontiers in Genetics, 2015
The $1,000 Genome project has been drawing increasing attention since its launch a decade ago. Nanopore sequencing, the third-generation, is believed to be one of the most promising sequencing technologies to reach four gold standards set for the $1,000 ...
Yue eWang, Qiuping eYang, Zhimin eWang
doaj   +3 more sources

Diagnostic value of a nanopore sequencing assay of bronchoalveolar lavage fluid in pulmonary tuberculosis

BMC Pulmonary Medicine, 2023
Background To determine the diagnostic accuracy of a nanopore sequencing assay of PCR products from a M. tuberculosis complex-specific region for testing of bronchoalveolar lavage fluid (BALF) samples or sputum samples from suspected pulmonary ...
Zhifeng Liu, Yang Yang, Qingfeng Wang, Lei Wang, Wenjuan Nie, Naihui Chu   +5 more
doaj   +2 more sources

NanoSTR: A method for detection of target short tandem repeats based on nanopore sequencing data

Frontiers in Molecular Biosciences, 2023
Short tandem repeats (STRs) are widely present in the human genome. Studies have confirmed that STRs are associated with more than 30 diseases, and they have also been used in forensic identification and paternity testing.
Jidong Lang, Zhihua Xu, Yue Wang, Jiguo Sun, Zhi Yang   +4 more
doaj   +2 more sources

Portable nanopore-sequencing technology: Trends in development and applications

Frontiers in Microbiology, 2023
Sequencing technology is the most commonly used technology in molecular biology research and an essential pillar for the development and applications of molecular biology.
Pin Chen, Zepeng Sun, Jiawei Wang, Xinlong Liu, Yun Bai, Jiang Chen, Anna Liu, Feng Qiao, Yang Chen, Chenyan Yuan, Jingjie Sha, Jinghui Zhang, Li-Qun Xu, Jian Li   +13 more
doaj   +2 more sources

Following the central dogma: Nanopore sequencing from DNA and RNA to proteins [PDF]

iScience
Summary: The first nanopore-based sequencer was launched in 2014, and subsequently, nanopore played an irreplaceable role in disclosing the first complete, gapless sequence of a human genome in 2022 due to its megabase-scale read lengths.
Yutong Wang, Qian Han, Yilong He, Shumin Deng, Ziwei Huang, Ziyan Zhang, Shuaijian Dai, Zhuxin Dong   +7 more
doaj   +2 more sources

The diagnostic value of Xpert detection combined with nanopore sequencing for tuberculosis in HIV/AIDS patients [PDF]

Infectious Medicine
Background: Tuberculosis (TB) remains a major cause of death in HIV/AIDS patients, where diagnosis is complicated by atypical presentation and paucibacillary disease. Current diagnostic methods have limitations in speed or sensitivity.
Chang Song, Chunyan Zhao, Dan Luo, Aichun Huang, Chaoyan Xu, Jieqing Zhong, Yujie Mo, Zhentao Huang, Xiaoshi Lin, Zhouhua Xie, Qingdong Zhu   +10 more
doaj   +2 more sources

Identifying and correcting repeat-calling errors in nanopore sequencing of telomeres

Genome Biology, 2022
Nanopore long-read sequencing is an emerging approach for studying genomes, including long repetitive elements like telomeres. Here, we report extensive basecalling induced errors at telomere repeats across nanopore datasets, sequencing platforms ...
Kar-Tong Tan, Michael K. Slevin, Matthew Meyerson, Heng Li   +3 more
doaj   +2 more sources