Results 21 to 30 of about 65,196 (347)

Nanopore Technology and Its Applications in Gene Sequencing

Biosensors, 2021
In recent years, nanopore technology has become increasingly important in the field of life science and biomedical research. By embedding a nano-scale hole in a thin membrane and measuring the electrochemical signal, nanopore technology can be used to ...
Bo Lin, Jianan Hui, Hongju Mao
doaj   +1 more source

Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification [PDF]

, 2020
Understanding genome organization and gene regulation requires insight into RNA transcription, processing and modification. We adapted nanopore direct RNA sequencing to examine RNA from a wild-type accession of the model plant Arabidopsis thaliana and a ...
Barton, Geoffrey J., Gould, Peter D., Hall, Anthony J. W., Knop, Katarzyna, Mackinnon, Katarzyna, Parker, Matthew T., Schurch, Nicholas J., Sherwood, Anna V., Simpson, Gordon G.   +8 more
core   +3 more sources

Genotyping of familial Mediterranean fever gene (MEFV)—Single nucleotide polymorphism—Comparison of Nanopore with conventional Sanger sequencing

PLoS ONE, 2022
Background Through continuous innovation and improvement, Nanopore sequencing has become a powerful technology. Because of its fast processing time, low cost, and ability to generate long reads, this sequencing technique would be particularly suitable ...
Jonas Schmidt, Sandro Berghaus, Frithjof Blessing, Holger Herbeck, Josef Blessing, Peter Schierack, Stefan Rödiger, Dirk Roggenbuck, Folker Wenzel   +8 more
doaj   +2 more sources

Adaptation of Oxford Nanopore technology for hepatitis C whole genome sequencing and identification of within-host viral variants

BMC Genomics, 2021
Background Hepatitis C (HCV) and many other RNA viruses exist as rapidly mutating quasi-species populations in a single infected host. High throughput characterization of full genome, within-host variants is still not possible despite advances in next ...
Nasir Riaz, Preston Leung, Kirston Barton, Martin A. Smith, Shaun Carswell, Rowena Bull, Andrew R. Lloyd, Chaturaka Rodrigo   +7 more
doaj   +1 more source

Direct oligonucleotide sequencing with nanopores [PDF]

Open Research Europe, 2020
Third-generation DNA sequencing has enabled sequencing of long, unamplified DNA fragments with minimal steps. Direct sequencing of ssDNA or RNA gives valuable insights like base-level modifications, phosphoramidite synthesis yield estimates and strand quality analysis, without the need to add the complimentary strand.
Sachin Chalapati, Conor A Crosbie, Dixita Limbachiya, Nimesh Pinnamaneni   +3 more
openaire   +2 more sources

Three decades of nanopore sequencing [PDF]

Nature Biotechnology, 2016
A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule through that pore. With the development of enzyme-based methods that ratchet polynucleotides through the nanopore, nucleobase-by-nucleobase, measurements of changes in the current ...
David, Deamer, Mark, Akeson, Daniel, Branton   +2 more
openaire   +2 more sources

Recent advances in nanopore sequencing [PDF]

ELECTROPHORESIS, 2012
The prospect of nanopores as a next‐generation sequencing platform has been a topic of growing interest and considerable government‐sponsored research for more than a decade. Oxford Nanopore Technologies recently announced the first commercial nanopore sequencing devices, to be made available by the end of 2012, while other companies (Life, Roche, and ...
Raj D, Maitra, Jungsuk, Kim, William B, Dunbar   +2 more
openaire   +2 more sources

Nanopore DNA sequencing with MspA [PDF]

Proceedings of the National Academy of Sciences, 2010
Nanopore sequencing has the potential to become a direct, fast, and inexpensive DNA sequencing technology. The simplest form of nanopore DNA sequencing utilizes the hypothesis that individual nucleotides of single-stranded DNA passing through a nanopore will uniquely modulate an ionic current flowing through the pore, allowing the record ...
Ian M, Derrington, Tom Z, Butler, Marcus D, Collins, Elizabeth, Manrao, Mikhail, Pavlenok, Michael, Niederweis, Jens H, Gundlach   +6 more
openaire   +2 more sources

Modification mapping by nanopore sequencing

Frontiers in Genetics, 2022
Next generation sequencing (NGS) has provided biologists with an unprecedented view into biological processes and their regulation over the past 2 decades, fueling a wave of development of high throughput methods based on short read DNA and RNA sequencing. For nucleic acid modifications, NGS has been coupled with immunoprecipitation, chemical treatment,
Laura K. White, Jay R. Hesselberth
openaire   +3 more sources

Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing [PDF]

, 2017
Compared with conventional methods, single-molecule real-time (SMRT) DNA sequencing exhibits longer read lengths than conventional methods, less GC bias, and the ability to read DNA base modifications.
Henley, Robert Y., Jadhav, Vivek, Korlach, Jonas, Larkin, Joseph, Wanunu, Meni   +4 more
core   +1 more source