Results 21 to 30 of about 64,518 (349)

Recent advances in nanopore sequencing [PDF]

ELECTROPHORESIS, 2012
The prospect of nanopores as a next‐generation sequencing platform has been a topic of growing interest and considerable government‐sponsored research for more than a decade. Oxford Nanopore Technologies recently announced the first commercial nanopore sequencing devices, to be made available by the end of 2012, while other companies (Life, Roche, and ...
Raj D, Maitra, Jungsuk, Kim, William B, Dunbar   +2 more
openaire   +2 more sources

MinION Analysis and Reference Consortium: Phase 1 data release and analysis [PDF]

, 2015
The advent of a miniaturized DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools.
Benedict Paten, Bonnie L. Brown, Camilla L.C. Ip, David A. Eccles, David Buck, Elizabeth M. Batty, Ewan Birney, Hans J. Jansen, Hugh E. Olsen, Jared T. Simpson, John M. Urban, John R. Tyson, Justin O'Grady, Mariateresa de Cesare, Matthew Loose, MinION Analysis and Reference Consortium, Miten Jain, Paolo Piazza, Richard M. Leggett, Rory J. Bowden, Sara Goodwin, Solomon Mwaigwisya, Terrance P. Snutch, Vadim Zalunin   +23 more
core   +2 more sources

Nanopore DNA sequencing with MspA [PDF]

Proceedings of the National Academy of Sciences, 2010
Nanopore sequencing has the potential to become a direct, fast, and inexpensive DNA sequencing technology. The simplest form of nanopore DNA sequencing utilizes the hypothesis that individual nucleotides of single-stranded DNA passing through a nanopore will uniquely modulate an ionic current flowing through the pore, allowing the record ...
Ian M, Derrington, Tom Z, Butler, Marcus D, Collins, Elizabeth, Manrao, Mikhail, Pavlenok, Michael, Niederweis, Jens H, Gundlach   +6 more
openaire   +2 more sources

Simulation of Nanopore Sequencing Signals Based on BiGRU

Sensors, 2020
Oxford Nanopore sequencing is an important sequencing technology, which reads the nucleotide sequence by detecting the electrical current signal changes when DNA molecule is forced to pass through a biological nanopore.
Weigang Chen, Peng Zhang, Lifu Song, Jinsheng Yang, Changcai Han   +4 more
doaj   +1 more source

Modification mapping by nanopore sequencing

Frontiers in Genetics, 2022
Next generation sequencing (NGS) has provided biologists with an unprecedented view into biological processes and their regulation over the past 2 decades, fueling a wave of development of high throughput methods based on short read DNA and RNA sequencing. For nucleic acid modifications, NGS has been coupled with immunoprecipitation, chemical treatment,
Laura K. White, Jay R. Hesselberth
openaire   +3 more sources

Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing [PDF]

, 2017
Compared with conventional methods, single-molecule real-time (SMRT) DNA sequencing exhibits longer read lengths than conventional methods, less GC bias, and the ability to read DNA base modifications.
Henley, Robert Y., Jadhav, Vivek, Korlach, Jonas, Larkin, Joseph, Wanunu, Meni   +4 more
core   +1 more source

Metagenomic identification of severe pneumonia pathogens in mechanically-ventilated patients: a feasibility and clinical validity study

Respiratory Research, 2019
Background Metagenomic sequencing of respiratory microbial communities for pathogen identification in pneumonia may help overcome the limitations of culture-based methods. We examined the feasibility and clinical validity of rapid-turnaround metagenomics
Libing Yang, Ghady Haidar, Haris Zia, Rachel Nettles, Shulin Qin, Xiaohong Wang, Faraaz Shah, Sarah F. Rapport, Themoula Charalampous, Barbara Methé, Adam Fitch, Alison Morris, Bryan J. McVerry, Justin O’Grady, Georgios D. Kitsios   +14 more
doaj   +1 more source

Application of nanopore adaptive sequencing in pathogen detection of a patient with Chlamydia psittaci infection

Frontiers in Cellular and Infection Microbiology, 2023
IntroductionNanopore sequencing has been widely used in clinical metagenomic sequencing for pathogen detection with high portability and real-time sequencing.
Yanfeng Lin, Yanfeng Lin, Yan Dai, Shuang Zhang, Shuang Zhang, Hao Guo, Lang Yang, Jinhui Li, Kaiying Wang, Ming Ni, Ming Ni, Zongqian Hu, Zongqian Hu, Leili Jia, Huiying Liu, Peng Li, Hongbin Song, Hongbin Song   +17 more
doaj   +1 more source

NanoSTR: A method for detection of target short tandem repeats based on nanopore sequencing data

Frontiers in Molecular Biosciences, 2023
Short tandem repeats (STRs) are widely present in the human genome. Studies have confirmed that STRs are associated with more than 30 diseases, and they have also been used in forensic identification and paternity testing.
Jidong Lang, Zhihua Xu, Yue Wang, Jiguo Sun, Zhi Yang   +4 more
doaj   +1 more source

Reversible positioning of single molecules inside zero-mode waveguides [PDF]

, 2014
We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to
Foquet, Mathieu, Korlach, Jonas, Larkin, Joseph, Turner, Stephen W., Wanunu, Meni   +4 more
core   +2 more sources