Results 51 to 60 of about 998,588 (389)

Models and information-theoretic bounds for nanopore sequencing

, 2018
Nanopore sequencing is an emerging new technology for sequencing DNA, which can read long fragments of DNA (~50,000 bases) in contrast to most current short-read sequencing technologies which can only read hundreds of bases. While nanopore sequencers can
Diggavi, Suhas, Kannan, Sreeram, Mao, Wei   +2 more
core   +1 more source

Detailed evaluation of data analysis tools for subtyping of bacterial isolates based on whole genome sequencing : Neisseria meningitidis as a proof of concept [PDF]

, 2019
Whole genome sequencing is increasingly recognized as the most informative approach for characterization of bacterial isolates. Success of the routine use of this technology in public health laboratories depends on the availability of well-characterized ...
Bertrand, Sophie, De Keersmaecker, Sigrid CJ, Marchal, Kathleen, Mattheus, Wesley, Roosens, Nancy HC, Saltykova, Assia   +5 more
core   +1 more source

Nanopore Sequencing Technology and Tools for Genome Assembly: Computational Analysis of the Current State, Bottlenecks and Future Directions

, 2018
Nanopore sequencing technology has the potential to render other sequencing technologies obsolete with its ability to generate long reads and provide portability.
Alkan, Can, Cali, Damla Senol, Ghose, Saugata, Kim, Jeremie S., Mutlu, Onur   +4 more
core   +2 more sources

Evaluation of Nanopore sequencing for Mycobacterium tuberculosis drug susceptibility testing and outbreak investigation: a genomic analysis

The Lancet Microbe, 2022
Summary Background Mycobacterium tuberculosis whole-genome sequencing (WGS) has been widely used for genotypic drug susceptibility testing (DST) and outbreak investigation.
M. Hall, M. Rabodoarivelo, A. Koch, A. Dippenaar, S. George, M. Grobbelaar, R. Warren, T. Walker, H. Cox, S. Gagneux, D. Crook, T. Peto, N. Rakotosamimanana, S. Grandjean Lapierre, Z. Iqbal   +14 more
semanticscholar   +1 more source

De novo whole-genome assembly of a wild type yeast isolate using nanopore sequencing [version 2; referees: 1 approved, 3 approved with reservations]

F1000Research, 2018
Background: The introduction of the MinION sequencing device by Oxford Nanopore Technologies may greatly accelerate whole genome sequencing. Nanopore sequence data offers great potential for de novo assembly of complex genomes without using other ...
Michael Liem, Hans J. Jansen, Ron P. Dirks, Christiaan V. Henkel, G. Paul H. van Heusden, Richard J.L.F. Lemmers, Trifa Omer, Shuai Shao, Peter J. Punt, Herman P. Spaink   +9 more
doaj   +1 more source

Application of Nanopore Sequencing for High Throughput Genotyping in Horses

Animals, 2023
Nanopore sequencing is a third-generation biopolymer sequencing technique that relies on monitoring the changes in an electrical current that occur as nucleic acids are passed through a protein nanopore.
Artur Gurgul, Igor Jasielczuk, Tomasz Szmatoła, Sebastian Sawicki, Ewelina Semik-Gurgul, Bogusława Długosz, Monika Bugno-Poniewierska   +6 more
doaj   +1 more source

Applications of Nanopore sequencing in precision cancer medicine

International Journal of Cancer
Oxford Nanopore Technologies sequencing, also referred to as Nanopore sequencing, stands at the forefront of a revolution in clinical genetics, offering the potential for rapid, long read, and real‐time DNA and RNA sequencing.
S. Dyshlovoy, Stefanie Paigin, Ann-Kristin Afflerbach, A. Lobermeyer, Stefan Werner, U. Schüller, Carsten Bokemeyer, A. Schuh, Lina Bergmann, G. von Amsberg, S. Joosse   +10 more
semanticscholar   +1 more source

Benchmarking of Nanopore R10.4 and R9.4.1 flow cells in single-cell whole-genome amplification and whole-genome shotgun sequencing.

Computational and Structural Biotechnology Journal, 2023
Third-generation sequencing can be used in human cancer genomics and epigenomic research. Oxford Nanopore Technologies (ONT) recently released R10.4 flow cell, which claimed an improved read accuracy compared to R9.4.1 flow cell. To evaluate the benefits
Y. Ni, Xudong Liu, Zemenu Mengistie Simeneh, Mengsu Yang, Runsheng Li   +4 more
semanticscholar   +1 more source

MethPhaser: methylation-based long-read haplotype phasing of human genomes

Nature Communications
The assignment of variants across haplotypes, phasing, is crucial for predicting the consequences, interaction, and inheritance of mutations and is a key step in improving our understanding of phenotype and disease.
Yilei Fu, Sergey Aganezov, Medhat Mahmoud, John Beaulaurier, Sissel Juul, Todd J. Treangen, Fritz J. Sedlazeck   +6 more
doaj   +1 more source

Correlation Dynamics and Enhanced Signals for Serial DNA Sequencing

, 2013
Nanopore based sequencing has demonstrated significant potential for the development of fast, accurate, and cost-efficient fingerprinting techniques for next generation molecular detection and sequencing.
Ahmed, Towfiq, Balatsky, Alexander V., Di Ventra, Massimiliano, Haraldsen, Jason T., Rehr, John J., Schuller, Ivan K.   +5 more
core   +1 more source