Results 61 to 70 of about 998,588 (389)

A single-molecule nanopore sequencing platform

bioRxiv
Nanopore sequencing, a third-generation sequencing technology, has revolutionized the gene sequencing industry with its advantages of long reads, fast speed, real-time sequencing and analysis, and potential in detecting base modifications.
Jiayuan Zhang, Yuning Zhang, Lele Wang, Fei Guo, Quanxin Yun, Tao Zeng, Xu Yan, Lei Yu, Lei Cheng, Wei Wu, Xiao Shi, Junyi Chen, Yuhui Sun, Jingnan Yang, Rongrong Guo, Xianda Zhang, L. Kong, Zongan Wang, Jun Yao, Yan-Ni Tan, Liuxin Shi, Zhen Zhao, Zhongwang Feng, Xiaopeng Yu, Chuang Li, Wuqiang Zhan, Yulin Ren, Fan Yang, Zhenjun Liu, Guang-Hui Fan, Weiliang Zhong, Dachang Li, Lei He, Yanwei Qi, Meng Zhang, Yening Zhu, Heng Chi, Ziyu Zhao, Zhuofan Wei, Ziqi Song, Yanmei Ju, Ruijin Guo, Liang Xiao, Xiumei Lin, Liang Chen, Chentao Yang, Qiye Li, Ou Wang, Xin Jin, Ming Ni, Wenwei Zhang, Longqi Liu, Ying Gu, Jian Wang, Yuxiang Li, Xun Xu, Yuliang Dong   +56 more
semanticscholar   +1 more source

Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons

Nature Communications, 2021
Circular RNA (circRNA) is a class of covalently joined non-coding RNAs with functional roles in a wide variety of cellular processes. Their composition shows extensive overlap with exons found in linear mRNAs making it difficult to delineate their ...
K. Rahimi, M. Venø, D. Dupont, J. Kjems
semanticscholar   +1 more source

Expanding applications for nanopore sequencing [PDF]

Nature Reviews Genetics, 2019
Two new studies report advances for nanopore-based long-read sequencing for characterizing complex human transcriptomes and for analysing human repetitive DNA regions.
openaire   +2 more sources

ATF4‐mediated stress response as a therapeutic vulnerability in chordoma

Molecular Oncology, EarlyView.
We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone, James Dunford, Eleanor Calcutt, Vicki Gamble, Filiz Senbabaoglu Aksu, Lorena Ligammari, Georgina Wherry, Giorgia Gaeta, John C. Christianson, Adrienne M. Flanagan, Udo Oppermann, Adam P. Cribbs   +11 more
wiley   +1 more source

High-Quality Genome-Scale Models From Error-Prone, Long-Read Assemblies

Frontiers in Microbiology, 2020
Advances in nanopore-based sequencing techniques have enabled rapid characterization of genomes and transcriptomes. An emerging application of this sequencing technology is point-of-care characterization of pathogenic bacteria.
Jared T. Broddrick, Richard Szubin, Charles J. Norsigian, Jonathan M. Monk, Bernhard O. Palsson, Mary N. Parenteau   +5 more
doaj   +1 more source

Polymer translocation through a nanopore under an applied external field [PDF]

, 2006
We investigate the dynamics of polymer translocation through a nanopore under an externally applied field using the 2D fluctuating bond model with single-segment Monte Carlo moves.
Alberts B., Chang D.-C., Darnell J., de Gennes P. G., Doi M., Guo L., Ilkka Huopaniemi, Kaifu Luo, See-Chen Ying, Tapio Ala-Nissila   +9 more
core   +2 more sources

Direct Nanopore Sequencing of Individual Full Length tRNA Strands

bioRxiv, 2021
We describe a method for direct tRNA sequencing using the Oxford Nanopore MinION. The principal technical advance is custom adapters that facilitate end-to-end sequencing of individual tRNA molecules.
Niki Thomas, V. Poodari, Miten Jain, Hugh E. Olsen, M. Akeson, Robin Abu-Shumays   +5 more
semanticscholar   +1 more source

Complementarity of Long‐Reads and Optical Mapping in Parkinson's Disease for Structural Variants

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Long‐read sequencing and optical genome mapping technologies have the ability to detect large and complex structural variants. This has led to the discovery of novel pathogenic variants in neurodegenerative movement disorders. Thus, we aimed to systematically compare the SV detection capabilities of OGM and ONT in Parkinson's disease.
André Fienemann, Theresa Lüth, Susen Schaake, Carolin Gabbert, Marius Möller, Hauke Busch, Katja Lohmann, Jonas A. Gustafson, Danny E. Miller, Kensuke Daida, Manabu Funayama, Nobutaka Hattori, Samia Ben Sassi, Faycel Hentati, Matthew J. Farrer, Kristian K. Ullrich, Christine Klein, Joanne Trinh   +17 more
wiley   +1 more source

Transcriptome analysis of interna rootlets of the rhizocephalan Parasacculina sinensis reveals potential mechanisms of parasite host control

BMC Genomics
Background The endoparasitic rhizocephalan Parasacculina sinensis has a radically simplified morphology and primarily infests decapods crustaceans. Rhizocephalan barnacles usually absorb nutrients from the host through a complex rootlet system (the ...
Wenjie Li, Yiheng Feng, Haocheng Liu, Niklas Dreyer, Yue Him Wong   +4 more
doaj   +1 more source

Evaluation of multiplex nanopore sequencing for Salmonella serotype prediction and antimicrobial resistance gene and virulence gene detection

Frontiers in Microbiology, 2023
In a previous study, Multiplex-nanopore-sequencing based whole genome sequencing (WGS) allowed for accurate in silico serotype prediction of Salmonella within one day for five multiplexed isolates, using both SISTR and SeqSero2.
Xingwen Wu, Hao Luo, Chongtao Ge, Feng Xu, Xiangyu Deng, Martin Wiedmann, Robert C. Baker, Abigail E. Stevenson, Guangtao Zhang, Silin Tang   +9 more
doaj   +1 more source