Results 71 to 80 of about 998,588 (389)

Single-molecule methylation profiles of cell-free DNA in cancer with nanopore sequencing

bioRxiv, 2022
Epigenetic characterization of cell-free DNA (cfDNA) is an emerging approach for detecting and characterizing diseases such as cancer. We developed a strategy using nanopore-based single-molecule sequencing to measure cfDNA methylomes.
Billy T. Lau, A. Almeda, Marie C Schauer, M. McNamara, Xiangqi Bai, Qingxi Meng, Mira Partha, S. Grimes, Hojoon Lee, G. Heestand, Hanlee P. Ji   +10 more
semanticscholar   +1 more source

Nanopore-Based Target Sequence Detection

PLOS ONE, 2016
The promise of portable diagnostic devices relies on three basic requirements: comparable sensitivity to established platforms, inexpensive manufacturing and cost of operations, and the ability to survive rugged field conditions. Solid state nanopores can meet all these requirements, but to achieve high manufacturing yields at low costs, assays must be
Trevor J Morin, Tyler Shropshire, Xu Liu, Kyle Briggs, Cindy Huynh, Vincent Tabard-Cossa, Hongyun Wang, William B Dunbar   +7 more
openaire   +4 more sources

Epilepsy‐Associated Variants of a Single SCN1A Codon Exhibit Divergent Functional Properties

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Pathogenic variants in SCN1A, which encodes the voltage‐gated sodium channel NaV1.1, are associated with multiple epilepsy syndromes exhibiting a range of clinical severity. SCN1A variants are reported in different syndromes, including Dravet syndrome, which is associated with loss‐of‐function, whereas neonatal/infantile‐onset ...
Lanie N. Liebovitz, Christopher H. Thompson, Linda C. Laux, Alfred L. George Jr.   +3 more
wiley   +1 more source

Oxford Nanopore R10.4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or reference polishing

Nature Methods, 2022
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes.
M. Sereika, R. Kirkegaard, S. Karst, T. Michaelsen, E. A. Sørensen, R. Wollenberg, M. Albertsen   +6 more
semanticscholar   +1 more source

Non Thermal‐Driven Photocatalytic Ammonia Decomposition at Near‐Room Temperature on a Plasmonic Nanocone Array

Advanced Functional Materials, EarlyView.
Plasmonic photocatalytic ammonia decomposition occurs at near‐room temperature on a plasmonic Au nanocone array under visible light illumination. The nanostructure efficiently harnesses plasmonic modes, leading to increased reaction rates upon plasmon decay.
Thanh‐Lam Bui, S. M. Hossein Hejazi, Jan Navrátil, Hana Kmentová, Petr Bábor, Olivier Henrotte, Luca Mascaretti, Matteo Bisetto, Stephen K. Sanders, Andrea Schirato, Michal Otyepka, Piotr Błoński, Tiziano Montini, Radek Zboril, Paolo Fornasiero, Alessandro Alabastri, Alberto Naldoni, Stepan Kment   +17 more
wiley   +1 more source

Accurate Detection of HPV Integration Sites in Cervical Cancer Samples Using the Nanopore MinION Sequencer Without Error Correction

Frontiers in Genetics, 2020
During the carcinogenesis of cervical cancer, the DNA of human papillomavirus (HPV) is frequently integrated into the human genome, which might be a biomarker for the early diagnosis of cervical cancer.
Wenjuan Yang, Ying Liu, Ruyi Dong, Jia Liu, Jidong Lang, Jialiang Yang, Weiwei Wang, Jingjing Li, Bo Meng, Geng Tian, Geng Tian   +10 more
doaj   +1 more source

Comparison of Single Molecule, Real-Time Sequencing and Nanopore Sequencing for Analysis of the Size, End-Motif, and Tissue-of-Origin of Long Cell-Free DNA in Plasma.

Clinical Chemistry, 2022
BACKGROUND Recent studies using single molecule, real-time (SMRT) sequencing revealed a substantial population of analyzable long cell-free DNA (cfDNA) in plasma.
Stephanie C. Y. Yu, J. Deng, Rong Qiao, S. Cheng, Wenlei Peng, S. L. Lau, L. Y. L. Choy, Tak Y. Leung, J. Wong, V. Wong, G. Wong, P. Jiang, R. Chiu, K. C. Chan, Y. Lo   +14 more
semanticscholar   +1 more source

Nanopore Sequencing with MspA [PDF]

Biophysical Journal, 2009
Nanopores may provide the basis for a high-speed and inexpensive de novo DNA sequencing technique that could revolutionize medical and biological science. In this technique, single-stranded DNA is electrophoretically translocated through a pore with inner dimensions similar to that of DNA.
Derrington, Ian, Butler, Tom, Pavlenok, M., Collins, Marcus, Niederweis, Michael, Gundlach, Jens   +5 more
openaire   +1 more source

Expanding Chemical Space of Nucleic Acid Nanoparticles for Tunable Antiviral‐Like Immunomodulatory Responses and Potent Adjuvant Activity

Advanced Functional Materials, EarlyView.
We introduce a nucleic acid nanoparticle (NANP) platform designed to be rrecognized by the human innate immune system in a regulated manner. By changing chemical composition while maintaining constant architectural parameters, we identify key determinants of immunorecognition enabling the rational design of NANPs with tunable immune activation profiles
Martin Panigaj, Hannah S. Newton, Jian Wang, Laxmi Pandey, Yelixza I. Avilla, Phong Nguyen, Morgan Chandler, Justin Halman, Yasmine Radwan, Stephanie Smith, Elijah Edmondson, Simone Difilippantonio, Chelsea Sanders, Bao Tran, Yongmei Zhao, Shaojun Xie, Edward Cedrone, Barry W. Neun, Meni Wanunu, Nikolay V. Dokholyan, Marina A. Dobrovolskaia, Kirill A. Afonin   +21 more
wiley   +1 more source

Comparative analysis of hybrid-SNP microarray and nanopore sequencing for detection of large-sized copy number variants in the human genome

Molecular Cytogenetics
Background Nanopore sequencing is a technology that holds great promise for identifying all types of human genome variations, particularly structural variations.
Catarina Silva, José Ferrão, Bárbara Marques, Sónia Pedro, Hildeberto Correia, Ana Valente, António Sebastião Rodrigues, Luís Vieira   +7 more
doaj   +1 more source