Results 71 to 80 of about 65,196 (347)

Megabase-scale methylation phasing using nanopore long reads and NanoMethPhase

Genome Biology, 2021
The ability of nanopore sequencing to simultaneously detect modified nucleotides while producing long reads makes it ideal for detecting and phasing allele-specific methylation. However, there is currently no complete software for detecting SNPs, phasing
Vahid Akbari, Jean-Michel Garant, Kieran O’Neill, Pawan Pandoh, Richard Moore, Marco A. Marra, Martin Hirst, Steven J. M. Jones   +7 more
doaj   +1 more source

Clinical metagenomics. [PDF]

, 2019
Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly moving from research to clinical laboratories. This emerging approach is changing
Chiu, Charles Y, Miller, Steven A
core  

ENDO-Pore: high-throughput linked-end mapping of single DNA cleavage events using nanopore sequencing [PDF]

, 2021
Oscar Enrique Torres Montaguth, Stephen Cross, Kincaid W.A. Ingram, Laura Lee, Fiona M. Diffin, Mark D. Szczelkun   +5 more
openalex   +1 more source

Atomically Revealing Bulk Point Defect Dynamics in Hydrogen‐Driven γ‐Fe2O3 → Fe3O4 → FeO Transformation

Advanced Functional Materials, EarlyView.
In situ TEM uncovers the atomic‐scale mechanisms underlying hydrogen‐driven γ‐Fe2O3→Fe3O4→FeO reduction. In γ‐Fe2O3, oxygen vacancies cluster around intrinsic Fe vacancies, leading to nanopore formation, whereas in Fe3O4, vacancy aggregation is suppressed, preserving a dense structure.
Yupeng Wu, Dongxiang Wu, Wenhui Zhu, Xiaobo Chen, Zhikang Zhou, Zhilu Liang, Shuonan Ye, Meng Li, Xiao Tong, Kim Kisslinger, Sooyeon Hwang, Dmitri Zakharov, Renu Sharma, Judith C. Yang, Guangwen Zhou   +14 more
wiley   +1 more source

A comparison of methods for detecting DNA methylation from long-read sequencing of human genomes

Genome Biology
Background Long-read sequencing can enable the detection of base modifications, such as CpG methylation, in single molecules of DNA. The most commonly used methods for long-read sequencing are nanopore developed by Oxford Nanopore Technologies (ONT) and ...
Brynja D. Sigurpalsdottir, Olafur A. Stefansson, Guillaume Holley, Doruk Beyter, Florian Zink, Marteinn Þ. Hardarson, Sverrir Þ. Sverrisson, Nina Kristinsdottir, Droplaug N. Magnusdottir, Olafur Þ. Magnusson, Daniel F. Gudbjartsson, Bjarni V. Halldorsson, Kari Stefansson   +12 more
doaj   +1 more source

Identifying and correcting repeat-calling errors in nanopore sequencing of telomeres

Genome Biology, 2022
Nanopore long-read sequencing is an emerging approach for studying genomes, including long repetitive elements like telomeres. Here, we report extensive basecalling induced errors at telomere repeats across nanopore datasets, sequencing platforms ...
Kar-Tong Tan, Michael K. Slevin, Matthew Meyerson, Heng Li   +3 more
doaj   +1 more source

Air monitoring by nanopore sequencing

ISME Communications
Abstract While the air microbiome and its diversity are essential for human health and ecosystem resilience, comprehensive air microbial diversity monitoring has remained rare, so that little is known about the air microbiome’s composition, distribution, or functionality.
Reska, T.T.M., Pozdniakova, S., Borràs, S., Perlas Puente,A., Sauerborn, E., Cañas, L., Schloter, M., Rodó, X., Wang, Y., Winkler, J.B., Schnitzler, J.-P., Urban, L.   +11 more
openaire   +3 more sources

Rapid resistome mapping using nanopore sequencing [PDF]

Nucleic Acids Research, 2016
AbstractThe emergence of antibiotic resistance in human pathogens has become a major threat to modern medicine and in particular hospitalized patients. The outcome of antibiotic treatment can be affected by the composition of the gut resistome either by enabling resistance gene acquisition of infecting pathogens or by modulating the collateral effects ...
van der Helm, Eric, Imamovic, Lejla, Hashim Ellabaan, Mostafa M, van Schaik, Willem, Koza, Anna, Sommer, Morten O. A.   +5 more
openaire   +3 more sources

A Biologically‐Architected Wear and Damage‐Resistant Nanoparticle Coating From the Radular Teeth of Cryptochiton stelleri

Advanced Functional Materials, EarlyView.
The ultrahard teeth of mollusks that feed on rocky substrates contain a wear‐resistant coating on their surfaces consisting of densely packed mesocrystalline magnetic nanoparticles within an organic matrix. These coatings display significant hardness and toughness through their highly controlled mesocrystalline architectures.
Taifeng Wang, Yu Chen, Ezra Sarmiento, Taige Hao, Atsushi Arakaki, Michiko Nemoto, Pablo Zavattieri, David Kisailus   +7 more
wiley   +1 more source

Primed and ready: nanopore metabarcoding can now recover highly accurate consensus barcodes that are generally indel-free

BMC Genomics
Background DNA metabarcoding applies high-throughput sequencing approaches to generate numerous DNA barcodes from mixed sample pools for mass species identification and community characterisation.
Jia Jin Marc Chang, Yin Cheong Aden Ip, Wan Lin Neo, Maxine A. D. Mowe, Zeehan Jaafar, Danwei Huang   +5 more
doaj   +1 more source