Results 111 to 120 of about 8,248,717 (265)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
, 2022 [ARCHIVES] Copyright Article From:Tropical Journal of Natural Product Research (TJNPR)Diabetes mellitus (DM) is linked to an increase in dyslipidemia, which is associated with atherosclerosis, one of the leading causes of cardiovascular ...
Wibisono, Nugroho +2 more
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Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Applied and Environmental MicrobiologyUnderstanding the gut microbiome’s intricate dynamics and its impact on host health necessitates the cultivation and isolation of its constituent microorganisms.
Sundar Hengoju +6 more
doaj +1 more source
A Validated Method for Standardization of the Bark of
Natural Product Communications, 2010 Clerodendron serratum (Linn.) Moon (family: Verbenaceae) is used in Indian System of Medicine for asthma and cough. In the present study the extract of the bark of the plant was standardized using oleanolic acid as a marker.
Arunava Gantait +4 more
doaj +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
FEBS Open Bio, EarlyView.Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
Nature CommunicationsLead halide perovskites are promising for artificial photosynthesis but suffer from aqueous instability. Here, we stabilize CsPbI3 quantum dots within a hydrophobic chlorine-functionalized covalent organic framework through multisite atomic-chlorine ...
Genping Meng +8 more
doaj +1 more source
Memòria del Parc Natural dels Ports 2024 [PDF]
Gestió efectuada durant el 2024 al Parc Natural dels Ports, destacar les accions estratègiques i les fites més rellevants en cada un dels àmbits i línies de treball.[es] Gestión efectuada durante el 2024 en el Parque Natural de Els Ports, destaca las ...
Parc Natural dels Ports
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