Comparative Evaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Human Brucellosis [PDF]
Archives of Razi Institute, 2021 Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. The primary diagnosis of brucellosis can be effective in the achievement of satisfying treatment results and prevention of chronic infections.
L Rahbarnia +3 more
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One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis [PDF]
Memorias do Instituto Oswaldo Cruz, 2002 Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors ...
Cribb Pamela +2 more
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Influenza A H5N1 Detection [PDF]
Emerging Infectious Diseases, 2005 We developed a sensitive and rapid real-time reverse transcription-polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples.
Enders K.O. Ng +4 more
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Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]
Veterinary World, 2017 Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal +6 more
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Nontuberculous mycobacterial conjunctival granuloma detected by nested polymerase chain reaction
Journal of the Formosan Medical Association, 2014 Nontuberculous mycobacteria (NTM) are unusual pathogens causing ocular infections, mostly seen as keratitis, scleral buckle infections, and implantation infections, which are usually related to ocular trauma, or surgery. Conjunctival NTM infections are even rarer.
Chi-Juei Jeng +3 more
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Detection of Helicobacter pylori glmM gene in bovine milk using Nested polymerase chain reaction [PDF]
Veterinary World, 2015 Aim: The aim was to detect the glmM gene of Helicobacter pylori (H. pylori) in cow’s milk from different dairy farms in Khartoum State using Nested polymerase chain reaction (PCR).
Eyman Y. Osman +5 more
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Evaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Clinically Suspected Tuberculous Pleurisy [PDF]
Journal of Clinical and Diagnostic Research, 2013 Background: Early diagnosis of tuberculosis is important in its control. The conventional techniques like smear microscopy and culture suffer from low sensitivity for diagnosis of extra-pulmonary tuberculosis like Pleural Tuberculosis (PTB) due to ...
Manmeet Kaur Gill +2 more
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Molecular Detection of Anaplasma bovis in Cattle from Central Part of Iran [PDF]
Veterinary Research Forum, 2010 Anaplasma bovis is a leukocytotropic agent of bovine anaplasmosis and there is no available information about molecular study on this agent in cattle of Iran. In this study a total 150 cattle blood samples were collected from central part of Iran.
Vahid Noaman, Parviz Shayan
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Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene
Emerging Infectious Diseases, 2001 We developed a sensitive nested polymerase chain reaction (PCR) procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested
Susana Pedraza-Díaz +3 more
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Jornal Brasileiro de Pneumologia, 2013 OBJECTIVE: To compare the performance of nested polymerase chain reaction (NPCR) with that of cultures in the detection of the Mycobacterium tuberculosis complex in pulmonary and extrapulmonary specimens.METHODS: We analyzed 20 and 78 pulmonary and ...
Adriana Antônia da Cruz Furini +8 more
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