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Current Pharmaceutical Design, 2019
Next-generation sequencing (NGS) is a powerful technology that allows for high-throughput multiplex sequencing. This chapter reviews the key steps in NGS wet lab and dry lab processing. The library preparation process prepares the substrates to be sequenced through nucleic acid isolation, fragmentation, isolation, end repair, adapter ligation, size ...
Martin Wiedmann, Laura M. Carroll
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Next-generation sequencing (NGS) is a powerful technology that allows for high-throughput multiplex sequencing. This chapter reviews the key steps in NGS wet lab and dry lab processing. The library preparation process prepares the substrates to be sequenced through nucleic acid isolation, fragmentation, isolation, end repair, adapter ligation, size ...
Martin Wiedmann, Laura M. Carroll
+6 more sources
Next Generation Sequencing: Transcriptomics
2021Fungal transcriptomics is a rising field which has gained attention in the last two decades. The recent democratization of high throughput next-generation sequencing (NGS) techniques for large-scale transcriptomic analysis provided the opportunity to explore transcriptomes at an unprecedented level of resolution.
Sillo, Fabiano
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Revue medicale de Bruxelles, 2018
Fine needle aspiration is the gold standard method to differentiate benign thyroid nodules from malignant. However, for 15 to 30% of the cases the cytological diagnosis is indeterminate, leading to surgery. Integration of new molecular markers is opening new perspectives in order to increase the diagnostic precision of thyroid nodules with an ...
M, Le Mercier +6 more
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Fine needle aspiration is the gold standard method to differentiate benign thyroid nodules from malignant. However, for 15 to 30% of the cases the cytological diagnosis is indeterminate, leading to surgery. Integration of new molecular markers is opening new perspectives in order to increase the diagnostic precision of thyroid nodules with an ...
M, Le Mercier +6 more
+5 more sources
Next-generation DNA sequencing
Nature Biotechnology, 2008DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection. Over the past three years, massively parallel DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by over two orders of magnitude, and democratizing the field by putting ...
Jay, Shendure, Hanlee, Ji
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Next-Generation Sequencing Platforms
Annual Review of Analytical Chemistry, 2013Automated DNA sequencing instruments embody an elegant interplay among chemistry, engineering, software, and molecular biology and have built upon Sanger's founding discovery of dideoxynucleotide sequencing to perform once-unfathomable tasks. Combined with innovative physical mapping approaches that helped to establish long-range relationships between
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Next generation sequencing technologies
Drug Discovery Today: Technologies, 2005From the investigation of disease-associated loci in humans, to monitoring the changing genomes of pathogenic viruses and bacteria, sequencing is a powerful and versatile tool. A new generation of sequencing technologies will increase the speed and lower the cost of sequencing, and promises to expand the utility of sequencing in drug discovery and ...
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