Results 101 to 110 of about 773 (139)

HUMAN NM23-H1-PROTEIN AND H2-PROTEIN HAVE SIMILAR NUCLEOSIDE DIPHOSPHATE KINASE-ACTIVITIES

International Journal of Oncology, 1992
The nucleoside diphosphate kinase activity of nm23-H1 and nm23-H2 proteins was examined. Full length nm23-H1 and nm23-H2 proteins were produced in E.coli in fusion form with a 26 kDa glutathione S-transferase (GST). Affinity purified nm23-H1 and nm23-H2 formed phosphoenzyme intermediates when incubated with [gamma-P-32]ATP.
T, Urano, S, Fushida, K, Furukawa, H, Shiku   +3 more
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Nuclear Localization of Nucleoside Diphosphate Kinase Type B (nm23-H2) in Cultured Cells

Experimental Cell Research, 1996
Nucleoside diphosphate (NDP) kinases are metabolic enzymes found ubiquitously in cells. Recently, two known human isoforms of NDP kinase (A and B), identical to the protein products of the genes nm23-H1 and nm23-H2, respectively, have been implicated in cancer metastasis and transcriptional regulation.
S K, Kraeft, F, Traincart, S, Mesnildrey, J, Bourdais, M, Véron, L B, Chen   +5 more
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Measurement of nucleoside diphosphate kinase—Nm23 activity by anion-exchange high-performance liquid chromatography

Journal of Chromatography B: Biomedical Sciences and Applications, 1994
A first-order assay to detect the activity of nucleoside diphosphate kinase (NDP-kinase; EC 2.7.4.6) was developed. In this assay, the activity of NDP-kinase is measured using various deoxy- and ribonucleotide triphosphates as phosphate donors and dADP as phosphate acceptor.
G, Pulido-Cejudo, J, Gagnon, J M, Leclerc, K, Jamison, J, Gordon, J, Campione-Piccardo   +5 more
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Nm23/nucleoside diphosphate kinase: Toward a structural and biochemical understanding of its biological functions

BioEssays, 1995
AbstractThe nm23 gene, a putative metastasis suppressor gene, was originally identified by its reduced expression in highly metastatic K‐1735 murine melanoma cell lines, as compared to related, low metastatic melanoma cell lines. Transfection of nm23 cDNA has been reported to suppress malignant progression in Drosophila and mammalian cells.
A, de la Rosa, R L, Williams, P S, Steeg
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nm23 -H4, a new member of the family of human nm23 /nucleoside diphosphate kinase genes localised on chromosome 16p13

Human Genetics, 1997
A novel human nm23/nucleoside diphosphate (NDP) kinase gene, called nm23-H4, was identified by screening a human stomach cDNA library with a probe generated by amplification by reverse transcription-polymerase chain reaction. The primers were designed from publicly available database cDNA sequences selected according to their homology to the human nn23-
L, Milon, M F, Rousseau-Merck, A, Munier, M, Erent, I, Lascu, J, Capeau, M L, Lacombe   +6 more
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Nucleoside diphosphate kinase/NM23 expression in breast cancer: Lack of correlation with lymph‐node metastasis

International Journal of Cancer, 1992
AbstractThe product of the nm23‐H1 gene, reported to be a meta‐static suppressor gene, was recently identified as the nucleoside diphosphate (NDP) kinase A, and was found to be over‐expressed in several types of malignant tumors as compared with normal tissues.
X, Sastre-Garau, M L, Lacombe, M, Jouve, M, Véron, H, Magdelénat   +4 more
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NM23/Nucleoside Diphosphate Kinase as a Transcriptional Activator of c-myc

1996
Expression of the nm23 gene has been correlated with a variety of cellular mechanisms including tumor metastasis (Steeg et al. 1988; Nakayama et al. 1992, 1993), neoplastic development (Zynick et al. 1993), normal development and differentiation (Dearolf et al. 1988; Biggs et al. 1988, Wallet et al. 1990; Yamashiro et al.
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In vivo cross-linking of nm23/nucleoside diphosphate kinase to the PDGF-A gene promoter

Molecular Biology Reports, 2003
Human isoforms A and B of nm23/nucleoside diphosphate (NDP) kinase, functionally important in development and cancer, have been reported to bind to DNA, and in particular isoform A to the PDGF-A promoter and isoform B to the c-myc promoter and to telomeric repeats. However, no direct proof of the binding in vivo has yet been obtained.
CERVONI, Laura, PIETRANGELI, Paola, CHICHIARELLI, Silvia, ALTIERI, Fabio, L. Egistelli, TURANO, Carlo, I. Lascu, GIARTOSIO, Anna   +7 more
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A novel human nucleoside diphosphate (NDP) kinase, Nm23-H6, localizes in mitochondria and affects cytokinesis

Journal of Cellular Biochemistry, 2000
Nucleoside diphosphate kinases (NDP kinases) are enzymes known to be conserved throughout evolution and have been shown to be involved in various biological events, in addition to the "housekeeping" phosphotransferase activity. We present the molecular cloning of a novel human NDP kinase gene, termed Nm23-H6.
H, Tsuiki, M, Nitta, A, Furuya, N, Hanai, T, Fujiwara, M, Inagaki, M, Kochi, Y, Ushio, H, Saya, H, Nakamura   +9 more
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A plant nucleoside diphosphate kinase homologous to the human Nm23 gene product: purification and characterization

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1994
Nucleoside diphosphate kinases (NDPKs) catalyze the transfer of high-energy phosphates from nucleoside triphosphates to nucleoside diphosphates and may be involved in the regulation of growth, development, and signal transduction processes. We report here the purification and characterization of NDPK from detergent-solubilized extracts of dark-grown ...
D, Sommer, P S, Song
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