Results 1 to 10 of about 262,938 (280)

Non-Canonical Amino Acid-Based Engineering of (R)-Amine Transaminase [PDF]

Frontiers in Chemistry, 2022
Non-canonical amino acids (ncAAs) have been utilized as an invaluable tool for modulating the active site of the enzymes, probing the complex enzyme mechanisms, improving catalytic activity, and designing new to nature enzymes.
Amol D. Pagar, Hyunwoo Jeon, Taresh P. Khobragade, Sharad Sarak, Pritam Giri, Seonga Lim, Tae Hyeon Yoo, Byoung Joon Ko, Hyungdon Yun   +8 more
doaj   +5 more sources

Non-canonical amino acid labeling in proteomics and biotechnology [PDF]

Journal of Biological Engineering, 2019
Metabolic labeling of proteins with non-canonical amino acids (ncAAs) provides unique bioorthogonal chemical groups during de novo synthesis by taking advantage of both endogenous and heterologous protein synthesis machineries.
Aya M. Saleh, Kristen M. Wilding, Sarah Calve, Bradley C. Bundy, Tamara L. Kinzer-Ursem   +4 more
doaj   +5 more sources

Photoregulation of PRMT-1 Using a Photolabile Non-Canonical Amino Acid [PDF]

Molecules, 2021
Protein methyltransferases are vital to the epigenetic modification of gene expression. Thus, obtaining a better understanding of and control over the regulation of these crucial proteins has significant implications for the study and treatment of ...
Elizabeth A. King, Emily M. Peairs, Diya M. Uthappa, Jordan K. Villa, Cameron M. Goff, Naya K. Burrow, Rebecca T. Deitch, Anna K. Martin, Douglas D. Young   +8 more
doaj   +5 more sources

Fluorescent non-canonical amino acid provides insight into the human serotonin transporter [PDF]

Nature Communications
The serotonin transporter (SERT), responsible for the reuptake of released serotonin, serves as a major target for antidepressants and psychostimulants. Nevertheless, refining the mechanistic models for SERT remains challenging.
Andreas Nygaard, Linda G. Zachariassen, Kathrine S. Larsen, Anders S. Kristensen, Claus J. Loland   +4 more
doaj   +6 more sources

Non-canonical amino acid incorporation into AAV5 capsid enhances lung transduction in mice [PDF]

Molecular Therapy: Methods & Clinical Development, 2023
Gene therapy using recombinant adeno-associated virus (rAAV) relies on safe, efficient, and precise in vivo gene delivery that is largely dependent on the AAV capsid.
Hao Chang, Ailing Du, Jun Jiang, Lingzhi Ren, Nan Liu, Xuntao Zhou, Jialing Liang, Guangping Gao, Dan Wang   +8 more
doaj   +2 more sources

Rapid Structural Analysis of a Synthetic Non-canonical Amino Acid by Microcrystal Electron Diffraction [PDF]

Frontiers in Molecular Biosciences, 2021
Structural characterization of small molecules is a crucial component of organic synthesis. In this work, we applied microcrystal electron diffraction (MicroED) to analyze the structure of the product of an enzymatic reaction that was intended to produce
Patrick R. Gleason, Patrick R. Gleason, Brent L. Nannenga, Brent L. Nannenga, Jeremy H. Mills, Jeremy H. Mills   +5 more
doaj   +2 more sources

Residue-Specific Incorporation of the Non-Canonical Amino Acid Norleucine Improves Lipase Activity on Synthetic Polyesters [PDF]

Frontiers in Bioengineering and Biotechnology, 2022
Environmentally friendly functionalization and recycling processes for synthetic polymers have recently gained momentum, and enzymes play a central role in these procedures.
Karolina Haernvall, Patrik Fladischer, Patrik Fladischer, Heidemarie Schoeffmann, Sabine Zitzenbacher, Tea Pavkov-Keller, Tea Pavkov-Keller, Tea Pavkov-Keller, Tea Pavkov-Keller, Karl Gruber, Karl Gruber, Karl Gruber, Michael Schick, Motonori Yamamoto, Andreas Kuenkel, Doris Ribitsch, Doris Ribitsch, Georg M. Guebitz, Georg M. Guebitz, Birgit Wiltschi, Birgit Wiltschi, Birgit Wiltschi   +21 more
doaj   +2 more sources

Design and evolution of artificial enzyme with in-situ biosynthesized non-canonical amino acid [PDF]

Nature Communications
The creation of designer enzymes by incorporating genetically encoded non-canonical amino acids (ncAAs) could significantly expand the catalytic repertoire of the enzyme universe for abiological transformations.
Wei Huang, Shiping Wang, Ya Wei, Yuting Bai, Zhixi Zhu, Dejing Yin, Tao Liu, Xiang Sheng, Zhi Zhou   +8 more
doaj   +2 more sources

Evidence for co-translational misincorporation of non-canonical amino acid hydroxyproline in recombinant antibodies produced in Chinese Hamster Ovary (CHO) cell lines. [PDF]

PLoS ONE, 2020
With the advent of highly sensitive technologies such as tandem mass spectrometry and next-generation sequencing, recombinant antibodies are now routinely analyzed for the presence of low-level sequence variants including amino acid misincorporations ...
Shanta Boddapati, Jason Gilmore, Kyle Boone, John Bushey, Jonathan Ross, Brian Gfeller, William McFee, Romesh Rao, Greg Corrigan, Aaron Chen, Howard Clarke, John Valliere-Douglass, Swapnil Bhargava   +12 more
doaj   +2 more sources

Development of orthogonal aminoacyl-tRNA synthetase mutant for incorporating a non-canonical amino acid [PDF]

AMB Express
Genetic code expansion involves introducing non-canonical amino acids (ncAAs) with unique functional groups into proteins to broaden their applications.
Dongheon Lee, Ja Gyung Kim, Tae Wan Kim, Jong-il Choi   +3 more
doaj   +2 more sources