Results 31 to 40 of about 262,938 (280)

The genetic incorporation of thirteen novel non-canonical amino acids [PDF]

Chem. Commun., 2014
Thirteen novel non-canonical amino acids were synthesized and tested for suppression of an amber codon using a mutant pyrrolysyl-tRNA synthetase–tRNAPylCUA pair.
Alfred, Tuley, Yane-Shih, Wang, Xinqiang, Fang, Yadagiri, Kurra, Yohannes H, Rezenom, Wenshe R, Liu   +5 more
openaire   +2 more sources

Lithographic Patterning of Photoreactive Cell-Adhesive Proteins [PDF]

, 2007
We describe a novel, simple method for the photolithographic patterning of cell-adhesive proteins. Intrinsically photoreactive proteins are synthesized in Escherichia coli through incorporation of the non-canonical, photosensitive amino acid para ...
Carrico, Isaac S., Franck, Christian, Heilshorn, Sarah C., Liu, Julie C., Maskarinec, Stacey A., Mock, Marissa L., Nowatzki, Paul J., Ravichandran, Guruswami, Tirrell, David A.   +8 more
core   +1 more source

Analyzing N-terminal Arginylation Through the Use of Peptide Arrays and Degradation Assays [PDF]

, 2016
Nα-terminal arginylation (Nt-arginylation) of proteins is mediated by the Ate1 arginyltransferase (R-transferase), a component of the Arg/N-end rule pathway.
Brower, Christopher, Piatkov, Konstantin, Varshavsky, Alexander, Wadas, Brandon   +3 more
core   +1 more source

Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila

Bio-Protocol, 2019
Copper-catalyzed azide-alkyne-cycloaddition (CuAAC), also known as ‘click chemistry’ serves as a technique for bio-orthogonal, that is, bio-compatible labeling of macromolecules including proteins or lipids.
Kathrin Marter, Oliver Kobler, Ines Erdmann, Elaheh Soleimanpour, Peter Landgraf, Anke Müller, Julia Abele, Ulrich Thomas, Daniela Dieterich   +8 more
doaj   +1 more source

Genome-Wide Identification, Functional Analysis and Expression Profiling of the Aux/IAA Gene Family in Tomato [PDF]

, 2012
Auxin is a central hormone that exerts pleiotropic effects on plant growth including the development of roots, shoots, flowers and fruit. The perception and signaling of the plant hormone auxin rely on the cooperative action of several components,among
Abel, Abel, Abel, Bargmann, Carole Bassa, Chaabouni, Chaabouni, Corinne Audran-Delalande, Deng, Dharmasiri, Dharmasiri, Dreher, Eshed, Farid Regad, Finn, Hagen, Hamilton, Isabelle Mila, Jain, Jones, Kagale, Kalluri, Kepinski, Kloosterman, Leclercq, Li, Liscum, Mohamed Zouine, Mondher Bouzayen, Montgomery, Nebenführ, Oeller, Ohme-Takagi, Ottenschläger, Overvoorde, Paponov, Pirrello, Reed, Remington, Saitou, Sato, Szemenyei, Tamura, Tan, Theologis, Tiwari, Tiwari, Uehara, Ulmasov, Ulmasov, Wang, Wang, Wang, Wang, Yamamoto, Zuckerkandl   +55 more
core   +4 more sources

β11-12 linker isomerization governs acid-sensing ion channel desensitization and recovery

eLife, 2020
Acid-sensing ion channels (ASICs) are neuronal sodium-selective channels activated by reductions in extracellular pH. Structures of the three presumptive functional states, high-pH resting, low-pH desensitized, and toxin-stabilized open, have all been ...
Matthew L Rook, Abby Williamson, John D Lueck, Maria Musgaard, David M Maclean   +4 more
doaj   +1 more source

Celiac Immunogenic Potential of α-Gliadin Epitope Variants from Triticum and Aegilops Species [PDF]

, 2019
The high global demand of wheat and its subsequent consumption arise from the physicochemical properties of bread dough and its contribution to the protein intake in the human diet.
Barro Losada, Francisco, Comino Montilla, Isabel María, López Casado, Miguel Ángel, Moreno Amador, María de Lourdes, Ozuna Serafini, Carmen Victoria, Ruiz Carnicer, Ángela, Segura Montero, Verónica, Sousa Martín, Carolina, Torres López, María Isabel   +8 more
core   +1 more source

Enhancing the performance of a mutant pyrrolysyl-tRNA synthetase to create a highly versatile eukaryotic cell-free protein synthesis tool

Scientific Reports, 2023
Modification of proteins with a broad range of chemical functionalities enables the investigation of protein structure and activity by manipulating polypeptides at single amino acid resolution.
Jeffrey L. Schloßhauer, Anne Zemella, Srujan K. Dondapati, Lena Thoring, Manpreet Meyer, Stefan Kubick   +5 more
doaj   +1 more source

Discovery of noncanonical translation initiation sites through mass spectrometric analysis of protein N termini [PDF]

, 2017
Translation initiation generally occurs at AUG codons in eukaryotes, although it has been shown that non-AUG or non-canonical translation initiation can also occur.
Barbhuiya, Mustafa, Eacker, Stephen, Halushka, Marc, Kim, Min-Sik, Menschaert, Gerben, Na, Chan Hyun, Overall, Christopher, Pandey, Akhilesh, Pletnikova, Olga, Troncoso, Juan, Verbruggen, Steven   +10 more
core   +1 more source

Facilitating the structural characterisation of non-canonical amino acids in biomolecular NMR [PDF]

Magnetic Resonance, 2022
Abstract. Peptides and proteins containing non-canonical amino acids (ncAAs) are a large and important class of biopolymers. They include non-ribosomally synthesised peptides, post-translationally modified proteins, expressed or synthesised proteins containing unnatural amino acids, and peptides and proteins that are chemically modified.
S. Kuschert, M. Stroet, Y. K.-Y. Chin, A. C. Conibear, X. Jia, T. Lee, C. R. O. Bartling, K. Strømgaard, P. Güntert, P. Güntert, P. Güntert, K. J. Rosengren, A. E. Mark, M. Mobli   +13 more
openaire   +6 more sources