Non-Canonical Amino Acids as Building Blocks for Peptidomimetics: Structure, Function, and Applications [PDF]
Biomolecules, 2023 This review provides a fresh overview of non-canonical amino acids and their applications in the design of peptidomimetics. Non-canonical amino acids appear widely distributed in nature and are known to enhance the stability of specific secondary ...
Tarsila G. Castro +4 more
doaj +2 more sources
Extensive breaking of genetic code degeneracy with non-canonical amino acids [PDF]
Nature Communications, 2023 Genetic code expansion (GCE) offers many exciting opportunities for the creation of synthetic organisms and for drug discovery methods that utilize in vitro translation.
Clinton A. L. McFeely +5 more
doaj +2 more sources
Facilitating the structural characterisation of non-canonical amino acids in biomolecular NMR [PDF]
Magnetic Resonance, 2023 Peptides and proteins containing non-canonical amino acids (ncAAs) are a large and important class of biopolymers. They include non-ribosomally synthesised peptides, post-translationally modified proteins, expressed or synthesised proteins containing ...
S. Kuschert +13 more
doaj +2 more sources
Protein Modification Employing Non-Canonical Amino Acids to Prepare SUMOylation Detecting Bioconjugates [PDF]
Pharmaceutics, 2022 Protein modification with non-canonical amino acids (ncAAs) represents a useful technology to afford homogenous samples of bioconjugates with site-specific modification.
Alexander C. Williard +10 more
doaj +2 more sources
Rosetta FlexPepDock to predict peptide-MHC binding: An approach for non-canonical amino acids. [PDF]
PLoS ONE, 2022 Computation methods that predict the binding of peptides to MHC-I are important tools for screening and identifying immunogenic antigens and have the potential to accelerate vaccine and drug development.
Nathaniel Bloodworth +4 more
doaj +4 more sources
AmpHGT: expanding prediction of antimicrobial activity in peptides containing non-canonical amino acids using multi-view constrained heterogeneous graph transformer [PDF]
BMC BiologyBackground Antimicrobial peptide (AMP) prediction has been extensively studied in recent years. However, many existing models do not fully leverage the intrinsic chemical structures of AMPs, such as atomic composition and sidechain group characteristics.
Yongcheng He +3 more
doaj +2 more sources
Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids [PDF]
Nature Communications, 2023 The 20 proteinogenic amino acids cannot always satisfy the requirements of protein engineering. Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant ...
Yunjun Pan +13 more
doaj +2 more sources
Computational Site Saturation Mutagenesis of Canonical and Non-Canonical Amino Acids to Probe Protein-Peptide Interactions [PDF]
Frontiers in Molecular Biosciences, 2022 Technologies for discovering peptides as potential therapeutics have rapidly advanced in recent years with significant interest from both academic and pharmaceutical labs.
Jeffrey K. Holden +4 more
doaj +2 more sources
Uncover New Reactivity of Genetically Encoded Alkyl Bromide Non-Canonical Amino Acids [PDF]
Frontiers in Chemistry, 2022 Genetically encoded non-canonical amino acids (ncAAs) with electrophilic moieties are excellent tools to investigate protein-protein interactions (PPIs) both in vitro and in vivo.
Xin Shu +8 more
doaj +2 more sources
Use of non‐canonical amino acids in genetic code expansion‐based therapeutics: Effects on mouse gut microbiota [PDF]
Microbial Biotechnology, 2023 Vaccines and cell therapeutics based on genetic code expansion are emerging. A crucial step in these therapeutic technologies is the oral administration of non‐canonical amino acids (ncAAs) to control pathogen growth and therapeutic protein levels in ...
Manyu Liang +7 more
doaj +2 more sources