Results 191 to 200 of about 10,520,469 (283)

A Rapid Lithium Ion‐Based Transformation Method for Kluyveromyces lactis

open access: yesYeast, EarlyView.
ABSTRACT Kluyveromyces lactis is a budding yeast host useful for recombinant protein production; however, its transformation often relies on electroporation due to the perceived low efficiency of chemical methods, thereby limiting throughput. In this study, we developed a robust, small‐scale chemical transformation protocol (using 15 μL of competent ...
Daisuke Tsugama, Ziyi Zuo
wiley   +1 more source

Nutrimental Determinants of Chronological Aging and Competitiveness in the snf1Δ Warburg Model

open access: yesYeast, EarlyView.
Deletion of SNF1 in Saccharomyces cerevisiae alters chronological aging in a nutrient‐dependent manner, shortening lifespan under low‐nutrient conditions and extending it under high‐glucose and high‐amino‐acid conditions. Under nutrient‐rich conditions, the snf1∆ strain also showed an approximately 2‐log increase in competitiveness relative to the wild
Abigail Correa‐Olivares   +7 more
wiley   +1 more source

Mapping Allergen B‐ and T‐Cell Epitopes: Technological Advances and Their Role in Precision Allergy Therapy

open access: yesAllergy, EarlyView.
ABSTRACT Allergic diseases arise from aberrant immune recognition of otherwise harmless environmental proteins and are driven by epitope‐specific interactions between allergens and the adaptive immune system. Although component‐resolved diagnostics have improved molecular characterisation of sensitization, they remain limited to whole‐allergen ...
Mark Møiniche   +11 more
wiley   +1 more source

The Use of Specific Non-Saccharomyces Yeasts as Sustainable Biocontrol Solutions Against Botrytis cinerea on Apples and Strawberries. [PDF]

open access: yesJ Fungi (Basel)
Gomomo Z   +8 more
europepmc   +1 more source

IgE‐Binding Bacterial Components Not Yet Documented in the Allergen Databases and Their Allergenic Mechanisms: An EAACI Position Paper

open access: yesAllergy, EarlyView.
ABSTRACT Among bacteria that are associated with the development and/or exacerbation of allergic/atopic diseases, several have been described to produce IgE‐binding components. However, only one bacterial protein, Bac s 1, is currently listed in the official database of the World Health Organization/International Union of Immunological Societies (WHO ...
Uta Jappe   +24 more
wiley   +1 more source

Two Metschnikowia nectar yeast species have similar volatile profiles but elicit differential foraging in bee pollinators

open access: yesEcological Entomology, EarlyView.
Yeasts that specialize in flower nectar play an important role in pollination ecology. Metschnikowia reukaufii and Metschnikowia koreensis were the most prevalent nectar yeasts found in our field sites. Bee pollinators exhibited different behavioural responses to nectar yeasts in field experiments. Bees visited more flowers with M.
M. Elizabeth Moore   +8 more
wiley   +1 more source

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