Results 81 to 90 of about 526,871 (218)

Adenylation Domain Engineering Enables Biosynthesis of an Improved Polymyxin Antibiotic

Angewandte Chemie, Volume 138, Issue 43, 19 October 2026.
To enable incorporation of l‐2‐aminodecanoic acid into polymyxin by fermentation, a single Trp‐to‐Ala substitution is rationally introduced into the adenylation‐domain residue of its nonribosomal peptide synthetase (NRPS) and efficiently switches substrate selectivity. The engineered strain produces biologically active Ada‐polymyxin, demonstrating that
Maximilian Müll, Pia Lanvers, Giulia Ravagnan, Kilian Klaus, Olena Nosovska, Ivan Vilotijevic, Jochen Schmid, Hajo Kries   +7 more
wiley   +2 more sources

Flexing and Stretching in Nonribosomal Peptide Synthetases

Chemistry & Biology, 2012
Re-engineering of nonribosomal peptide synthetase molecular assembly lines has been hampered by a lack of detailed knowledge concerning inter-domain substrate transfer. Recent structural studies of catalytically relevant domain-domain interactions provide valuable insights into this problem (Liu et al., 2011; Sundlov et al., 2012 [in this issue of ...
Pfennig, Sabrina, Stubbs, Milton T.
openaire   +2 more sources

Expanding the Chemical Space of Nonribosomal Peptide Synthetase-like Enzymes by Domain and Tailoring Enzyme Recombination

, 2018
The potential of tailoring enzymes in combination with engineered hybrid nonribosomal peptide synthetase-like enzymes was explored and resulted in methylated and prenylated forms of novel natural products, highlighting the possibilities and limitations ...
Clay C. C. Wang, Johannes W. A. van Dijk, Clay C. C. Wang (313310), Johannes W. A. van Dijk (3384221)   +3 more
core   +1 more source

Hybrid Macrocyclic Peptides — Synthetic Strategies to Diversify and Cyclize Peptide Libraries in Phage Display Selections

Chemistry – A European Journal, EarlyView.
Hybrid macrocyclic peptides unite genetically encoded peptide libraries with the structural complexity of synthetic small molecules. This Review critically analyzes phage‐compatible cyclization and diversification chemistries, highlights emerging opportunities beyond traditional cysteine‐based approaches, and outlines how future advances may enable the
Titia Rixt Oppewal, Clemens Mayer
wiley   +1 more source

Automated genome mining for natural products

BMC Bioinformatics, 2009
Background Discovery of new medicinal agents from natural sources has largely been an adventitious process based on screening of plant and microbial extracts combined with bioassay-guided identification and natural product structure elucidation ...
Zajkowski James, Ung Peter MU, Li Michael HT, Garneau-Tsodikova Sylvie, Sherman David H   +4 more
doaj   +1 more source

Taxonomic Positions of a Nyuzenamide-Producer and Its Closely Related Strains

Microorganisms, 2022
Streptomyces sp. N11-34 is a producer of bicyclic peptides named nyuzenamides A and B. We elucidated its taxonomic position and surveyed its nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) gene clusters by whole genome analysis ...
Hisayuki Komaki, Yasuhiro Igarashi, Tomohiko Tamura   +2 more
doaj   +1 more source

[Biologically active nonribosomal peptides. III. Mechanism of biosynthesis of nonribosomal peptides].

Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic], 2013
The third part of the review is concerned with investigation of nonribosomal peptides.
T. I. Orlova, V. G. Bulgakova, A. N. Polin   +2 more
openaire   +2 more sources

Naphthyridinomycin Biosynthesis Revealing the Use of Leader Peptide to Guide Nonribosomal Peptide Assembly

, 2016
Analysis of naphthyridinomycin gene cluster revealed that this antibiotic is generated by nonribosomal peptide synthetase (NRPS) machinery. However, four modules encoded by two genes do not correspond with the structural units in the final product ...
Yue Zhang (30585), Jin-Yue Pu (1934254), Man-Cheng Tang (1493491), Li-Qiang Song (1934251), Jian-Ping Guo (412563), Qiang Hua (114970), Gong-Li Tang (1829416), Chao Peng (686643)   +7 more
core   +1 more source

Antibacterial Property Of A Coral-Associated Bacterium Pseudoalteromonas Luteoviolacea Against Shrimp Pathogenic Vibrio Harveyi(In Vitro Study) [PDF]

, 2004
A coral-associated bacterium was successfully screened for secondary metabolites production based on PCR amplification of the non-ribosomal peptide synthetase gene and was identified as closely related to Pseudoalteromonas luteoviolacea based on its 16S ...
Grossart, Hans-Peter, Sabdono, Agus, Bachtiar, Tonny, Karna Radjasa, Ocky, Marten, Torben, Simon, Meinhard   +5 more
core   +2 more sources

ExMODE: A comprehensive resource for extremophile genomic and functional exploration

iMetaOmics, EarlyView.
ExMODE (https://db.genomics.cn/exmode/) integrates 3518 samples to build a unified extremophile resource, which hosts 1.35 billion habitat‐specific non‐redundant genes, 5.25 million representative protein structures, 67,026 metagenome‐assembled genomes (MAGs), and 164,132 biosynthetic gene clusters (BGCs). By combining sequence‐, structure‐, and genome‐
Denghui Li, Kailong Ma, Xiaoqiang Li, Jieni Wang, Zhen Cui, Yulu Zhang, Weiwen Wang, Jiawei Tong, Yang Guo, Zongan Wang, Peiyun Zeng, Jieyu Wang, Xiaokai Xu, Nannan Zhang, Yingying Zhang, Jing Chen, Qingjiang Hu, Wenzhen Yang, Zhiyong Li, Tao Yang, Wensi Du, Zhicheng Xu, Zhen Yue, Jian Wang, Guangyi Fan, Weijia Zhang, Xun Xu, Liujie Huo, Xiaofeng Wei, Liang Meng, Shanshan Liu   +30 more
wiley   +1 more source