Structures and function of a tailoring oxidase in complex with a nonribosomal peptide synthetase module [PDF]
Nature Communications, 2022 Nonribosomal peptide synthetases work with additional enzymes to synthesise secondary metabolites and therapeutics. Here, the authors explore bacillamide D synthesis and show the oxidase action is done while the intermediate is attached to the synthetase
Camille Marie Fortinez +5 more
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Polyketide Synthase and Nonribosomal Peptide Synthetase Gene Clusters in Type Strains of the Genus Phytohabitans [PDF]
Life, 2020 (1) Background: Phytohabitans is a recently established genus belonging to rare actinomycetes. It has been unclear if its members have the capacity to synthesize diverse secondary metabolites.
Hisayuki Komaki, Tomohiko Tamura
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Activity-based protein profiling of a surfactin-producing nonribosomal peptide synthetase in Bacillus subtilis [PDF]
STAR Protocols, 2022 Summary: We present an in vitro and in-cell activity-based protein profiling (ABPP) protocol for endogenous nonribosomal peptide synthetases (NRPSs).
Fumihiro Ishikawa +3 more
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Structures of teixobactin-producing nonribosomal peptide synthetase condensation and adenylation domains [PDF]
Current Research in Structural Biology, 2020 The recently discovered antibiotic teixobactin is produced by uncultured soil bacteria. The antibiotic inhibits cell wall synthesis of Gram-positive bacteria by binding to precursors of cell wall building blocks, and therefore it is thought to be less ...
Kemin Tan +7 more
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In Vivo Quantification of Surfactin Nonribosomal Peptide Synthetase Complexes in Bacillus subtilis [PDF]
MicroorganismsSurfactin, a potent biosurfactant produced by Bacillus subtilis, is synthesized using a non-ribosomal peptide synthetase (NRPS) encoded by the srfAA-AD operon.
Maliheh Vahidinasab +6 more
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The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase [PDF]
Nature Communications, 2019 The antimicrobial β-lactone obafluorin is produced by a Nonribosomal Peptide Synthetase (NRPS). Here the authors present the crystal structure of the obafluorin NRPS and develop a reconstitution assay that allows them to analyse product formation from ...
Dale F. Kreitler +4 more
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Catalytic trajectory of a dimeric nonribosomal peptide synthetase subunit with an inserted epimerase domain [PDF]
Nature Communications, 2022 The catalytic domains in nonribosomal peptide synthetases (NRPSs) are responsible for a choreography of events that elongates substrates into natural products.
Jialiang Wang +9 more
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Diversity of Nonribosomal Peptide Synthetase Genes in the Microbial Metagenomes of Marine Sponges
Marine Drugs, 2012 Genomic mining revealed one major nonribosomal peptide synthetase (NRPS) phylogenetic cluster in 12 marine sponge species, one ascidian, an actinobacterial isolate and seawater.
Ute Hentschel +3 more
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Structure, Function and Engineering of the Nonribosomal Peptide Synthetase Condensation Domain [PDF]
International Journal of Molecular SciencesZhenkuai Huang
exaly +2 more sources
BioCAT: Search for biosynthetic gene clusters producing nonribosomal peptides with known structure
Computational and Structural Biotechnology Journal, 2022 Nonribosomal peptides are a class of secondary metabolites synthesized by multimodular enzymes named nonribosomal peptide synthetases and mainly produced by bacteria and fungi.
Dmitry N. Konanov +3 more
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