Results 31 to 40 of about 557,797 (165)

Distinct roles for intra- and extracellular siderophores during Aspergillus fumigatus infection.

PLoS Pathogens, 2007
Siderophore biosynthesis by the highly lethal mould Aspergillus fumigatus is essential for virulence, but non-existent in humans, presenting a rare opportunity to strategize therapeutically against this pathogen.
Markus Schrettl, Elaine Bignell, Claudia Kragl, Yasmin Sabiha, Omar Loss, Martin Eisendle, Anja Wallner, Herbert N Arst, Ken Haynes, Hubertus Haas   +9 more
doaj   +1 more source

Structure of a bound peptide phosphonate reveals the mechanism of nocardicin bifunctional thioesterase epimerase-hydrolase half-reactions

Nature Communications, 2019
NocTE is a nonribosomal peptide synthetase thioesterase that completes the biosynthesis of pro-nocardicin G, the precursor for nocardicin β-lactam antibiotics. Here the authors provide mechanistic insights into NocTE by determining its crystal structures
Ketan D. Patel, Felipe B. d’Andrea, Nicole M. Gaudelli, Andrew R. Buller, Craig A. Townsend, Andrew M. Gulick   +5 more
doaj   +1 more source

Genetic and functional characterization of the gene cluster directing the biosynthesis of putisolvin I and II in Pseudomonas putida strain PCL1445 [PDF]

, 2008
Pseudomonas putida PCL1445 secretes two cyclic lipopeptides, putisolvin I and putisolvin II, which possess a surface-tension-reducing ability, and are able to inhibit biofilm formation and to break down biofilms of Pseudomonas species including ...
Dubern, J.F., Stiekema, W.J., Coppoolse, E.R., Bloemberg, G.V., Dubern JF, Coppoolse ER, Bloemberg GV, Stiekema WJ   +7 more
core   +1 more source

Systematic analysis of the kalimantacin assembly line NRPS module using an adapted targeted mutagenesis approach

MicrobiologyOpen, 2016
Kalimantacin is an antimicrobial compound with strong antistaphylococcal activity that is produced by a hybrid trans‐acyltransferase polyketide synthase/nonribosomal peptide synthetase system in Pseudomonas fluorescens BCCM_ID9359.
Birgit Uytterhoeven, Kenny Appermans, Lijiang Song, Joleen Masschelein, Thomas Lathouwers, Chris W. Michiels, Rob Lavigne   +6 more
doaj   +1 more source

Structural insights into the substrate-bound condensation domains of non-ribosomal peptide synthetase AmbB

Scientific Reports, 2022
Non-ribosomal peptide synthetases (NRPS) are multi-modular/domain enzymes that catalyze the synthesis of bioactive peptides. A crucial step in the process is peptide elongation accomplished by the condensation (C) domain with the aid of a peptidyl ...
Melissa-Jane Chu Yuan Kee, Sakshibeedu R. Bharath, Sheena Wee, Matthew W. Bowler, Jayantha Gunaratne, Shenquan Pan, Lianhui Zhang, Haiwei Song   +7 more
doaj   +1 more source

Adenylation Domain Engineering Enables Biosynthesis of an Improved Polymyxin Antibiotic

Angewandte Chemie, Volume 138, Issue 43, 19 October 2026.
To enable incorporation of l‐2‐aminodecanoic acid into polymyxin by fermentation, a single Trp‐to‐Ala substitution is rationally introduced into the adenylation‐domain residue of its nonribosomal peptide synthetase (NRPS) and efficiently switches substrate selectivity. The engineered strain produces biologically active Ada‐polymyxin, demonstrating that
Maximilian Müll, Pia Lanvers, Giulia Ravagnan, Kilian Klaus, Olena Nosovska, Ivan Vilotijevic, Jochen Schmid, Hajo Kries   +7 more
wiley   +2 more sources

Peptaibols: Diversity, bioactivity, and biosynthesis

Engineering Microbiology, 2022
Peptaibols are a large family of linear, amphipathic polypeptides consisting of 5-20 amino acid residues generated from the fungal nonribosomal peptide synthetase (NRPS) pathway.
Xuewen Hou, Ruonan Sun, Yanyan Feng, Runfang Zhang, Tianjiao Zhu, Qian Che, Guojian Zhang, Dehai Li   +7 more
doaj   +1 more source

Taxonogenomic Analysis of Marine-Derived Streptomyces sp. N11-50 and the Profile of NRPS and PKS Gene Clusters

Hydrobiology, 2023
Streptomyces sp. N11-50 was isolated from deep-sea water and found to produce diketopiperazine (DKP) compounds such as albonoursin and cyclo(Phe-Leu).
Hisayuki Komaki, Yasuhiro Igarashi, Tomohiko Tamura   +2 more
doaj   +1 more source

Biosynthesis of Tetronates by a Nonribosomal Peptide Synthetase–Polyketide Synthase System

, 2023
A cryptic tetronate biosynthetic pathway was identified in Kitasatospora niigatensis DSM 44781 via heterologous expression. Distinct from the currently known biosynthetic pathways, this system utilizes a partially functional nonribosomal peptide ...
Wenya Tian (4062244), Guangzheng Wei (4335901), Jun Zhang (48506), Mengmeng Zheng (1922920), Xinru Chen (6430319), Zixin Deng (31222), Xudong Qu (133214)   +6 more
core   +1 more source

A Fungal Nonribosomal Peptide Synthetase Module that can Synthesize Thiopyrazines

, 2016
A nonribosomal peptide synthetase-like enzyme (NRPS325) from Aspergillus terreus was reconstituted in vitro and was shown to synthesize thiopyrazines using an unprecedented mechanism. Substrate promiscuity of NRPS325 toward different amino acids and free
Neil Garg (2216656), Wenjun Zhang (685002), Kangjian Qiao (1458310), Yi Tang (55726), Hui Zhou (13228), Wei Xu (28953)   +5 more
core   +1 more source