Results 71 to 80 of about 463,256 (209)

Dereplication and de novo sequencing of nonribosomal peptides [PDF]

Nature Methods, 2009
Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP 'dereplication' and sequencing. We used multistage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also developed an algorithm for comparative NRP dereplication that establishes
Ng, Julio, Bandeira, Nuno, Liu, Wei-Ting, Ghassemian, Majid, Simmons, Thomas L., Gerwick, William, Linington, Roger, Dorrestein, Pieter, Pevzner, Pavel   +8 more
openaire   +2 more sources

Reengineering and Discovery of Nonribosomal Peptide Synthetases [PDF]

, 2010
Nonribosomal peptides are an important class of natural products including the life-saving antibiotics penicillin and vancomycin and the deadly microcystins and cereulide. This important category of natural products is thus a focus of many research labs
Evans, Bradley S.
core  

Novel chemical probes for the investigation of nonribosomal peptide assembly [PDF]

, 2017
Chemical probes were devised and evaluated for the capture of biosynthetic intermediates involved in the bio-assembly of the nonribosomal peptide echinomycin. Putative intermediate peptide species were isolated and characterised, providing fresh insights
Manuela Tosin, Y. T. C. Ho, Bushell, Dexter P., Ho, Y. T. Candace, Ghiringhelli, Francesca, Havemann, Judith, O. Köstner, Wilkening, Ina, E. Riva, Daniel J. Leng, Elena Riva, Riva, Elena, Judith Havemann, Francesca Ghiringhelli, J. Havemann, Otto Köstner, Leng, Daniel J., Passarella, Daniele, Dexter P. Bushell, I. Wilkening, Y. T. Candace Ho, D. P. Bushell, D. Passarella, M. Tosin, Daniele Passarella, F. Ghiringhelli, Ina Wilkening, Tosin, Manuela, Köstner, Otto, D. J. Leng   +29 more
core   +1 more source

The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase

Nature Communications, 2019
The antimicrobial β-lactone obafluorin is produced by a Nonribosomal Peptide Synthetase (NRPS). Here the authors present the crystal structure of the obafluorin NRPS and develop a reconstitution assay that allows them to analyse product formation from ...
Dale F. Kreitler, Erin M. Gemmell, Jason E. Schaffer, Timothy A. Wencewicz, Andrew M. Gulick   +4 more
doaj   +1 more source

Diversity of Linear Non-Ribosomal Peptide in Biocontrol Fungi

Journal of Fungi, 2020
Biocontrol fungi (BFs) play a key role in regulation of pest populations. BFs produce multiple non-ribosomal peptides (NRPs) and other secondary metabolites that interact with pests, plants and microorganisms. NRPs—including linear and cyclic peptides (L-
Xiaoyan Niu, Narit Thaochan, Qiongbo Hu
doaj   +1 more source

Repurposing FDA‐Approved Drugs to Inhibit Fungal PPTases for Broad‐Spectrum Synergy

Advanced Science, EarlyView.
Fungal secondary metabolites serve as crucial virulence effectors; however, the potential of their biosynthetic pathways as antifungal targets remains inadequately comprehended. Repurposed FDA‐approved drugs inhibit fungal PPTase (essential for secondary metabolism) in vivo.
Zili Song, Can Zhao, Qiaoling Wang, Hongjiao Zhang, Xiao Liu, Linqi Wang, Michael Bromley, Wen‐Bing Yin   +7 more
wiley   +1 more source

Designing and implementing an assay for the detection of rare and divergent NRPS and PKS clones in European, Antarctic and Cuban soils [PDF]

, 2015
The ever increasing microbial resistome means there is an urgent need for new antibiotics. Metagenomics is an underexploited tool in the field of drug discovery. In this study we aimed to produce a new updated assay for the discovery of biosynthetic gene
David A Pearce, Gregory C A Amos, Gregory C. A. Amos (799040), Leo Calvo-Bado (507757), Berry, Andrew E., Chiara Borsetto (799041), Andrew E Berry, Laskaris, Paris, Calvo-Bado, Leo, Paris Laskaris, Carlos Vallin (5665333), Berry, Andrew, Calvo-Bado, Leo A., David A. Pearce (189743), Leo Calvo-Bado, Elizabeth M H Wellington, Chiara Borsetto, Elizabeth M. H. Wellington (325617), Borsetto, Chiara, Kevin K Newsham, Amos, Gregory, Carlos Vallin, Paris Laskaris (325613), Andrew E. Berry (799043), Martin Krsek, Wellington, Elizabeth M. H., Martin Krsek (799042), Pearce, David, Pearce, David A., Kevin K. Newsham (352778), Vallin, Carlos, Krsek, Martin, Wellington, Elizabeth, Newsham, Kevin, Wellington, E. M. H., Amos, Gregory C. A., Newsham, Kevin K.   +36 more
core   +1 more source

Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters in Mucoromycotina Genomes

Journal of Fungi, 2021
Early-diverging fungi harbour unprecedented diversity in terms of living forms, biological traits and genome architecture. Before the sequencing era, non-Dikarya fungi were considered unable to produce secondary metabolites (SM); however, this ...
Grzegorz Koczyk, Julia Pawłowska, Anna Muszewska   +2 more
doaj   +1 more source

Structural Biology of Nonribosomal Peptide Synthetases [PDF]

, 2016
The nonribosomal peptide synthetases are modular enzymes that catalyze synthesis of important peptide products from a variety of standard and non-proteinogenic amino acid substrates. Within a single module are multiple catalytic domains that are responsible for incorporation of a single residue. After the amino acid is activated and covalently attached
Bradley R, Miller, Andrew M, Gulick
openaire   +2 more sources

Structural and Functional Insights into a Peptide Bond-Forming Bidomain from a Nonribosomal Peptide Synthetase [PDF]

, 2007
SummaryThe crystal structure of the bidomain PCP-C from modules 5 and 6 of the nonribosomal tyrocidine synthetase TycC was determined at 1.8 Å resolution.
Marahiel, Mohamed A., Samel, Stefan A., Schoenafinger, Georg, Essen, Lars-Oliver, Knappe, Thomas A.   +4 more
core   +1 more source