Results 1 to 10 of about 54,464 (302)
Protocol to detect m22G modification on single tRNA iso-decoders by immuno-northern blotting [PDF]
STAR ProtocolsSummary: tRNA modifications are highly dynamic and play crucial roles in regulating gene expression. Here, we present an assay for analyzing N2, N2-dimethylguanosine (m22G) modifications on tRNAs using an antibody-based detection approach.
Sseu-Pei Hwang +2 more
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Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs [PDF]
Nucleic Acids Research, 2017 Antony M Jose
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Methods Enzymol, 2013 To measure the size and amount of RNA transcribed from a specific gene of interest.
Shan L, He, Rachel, Green
europepmc +4 more sources
Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. [PDF]
PLoS ONE, 2015 The biological roles of RNA modifications are still largely not understood. Thus, developing a method for detecting RNA modifications is important for further clarification.
Eikan Mishima +13 more
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Proceedings of the Nutrition Society, 1996 Northern blotting is the main method for examining the expression of genes through measurement of an mRNA. Tissue specificity and the factors which regulate expression can both be determined by Northern blotting. A cDNA radioactively labelled with 32P is the most-commonly-used hybridization probe.
Paul Trayhurn
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Highly Sensitive Northern Hybridization Using a Rapid Protocol for Downward Alkaline Blotting of RNA
BioTechniques, 1998 A simple and fast RNA gel blot procedureis described that uses 50 mM NaOH tosimultaneously transfer and fix RNA to apositively charged nylon membrane. TheRNA is transferred in a downward direction,and transfer is routinely completedwithin 2.5 h.
Ivan L. Ingelbrecht +2 more
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Detection and Verification of Mammalian Mirtrons by Northern Blotting [PDF]
Methods Mol Biol, 2018 Mohammad Farid Zia, Alex S. Flynt
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Northern Blot Protocol v1 [PDF]
, 2020 Northern blot is a hybridization technique to visualize and quantify RNAs from cells or tissues using a radio-labeled probe. mRNAs only constitute a small fraction of total RNA in cells. Through enrichment of poly(A)-tailed mRNAs, this protocol provides increased sensitivity for the detection of lowly expressed mRNAs.
Sibylle Mitschka, Christine Mayr
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Validation and characterization of murine gammaherpesvirus 68 antisense transcripts by northern blot analysis and quantitative reverse transcription-PCR [PDF]
Archives of Biological Sciences, 2023 The transcription of mammalian genomes exhibits an intriguing complexity and numerous novel RNA molecules have been identified. Viruses with large DNA genomes, especially herpesviruses, generate many different RNA species, including long non-coding RNAs (
Kara Mehmet
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