Results 11 to 20 of about 54,464 (302)
Protocol to analyze tRNA and rRNA processing using biotin-labeled probes [PDF]
STAR ProtocolsSummary: Mature tRNAs and rRNAs are derived from larger precursor transcripts through intricate endo- and exonuclease processing steps. Here, we present a protocol to analyze rRNA and tRNA processing using total RNA extracted from human cells.
Sseu-Pei Hwang +2 more
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Current Issues in Molecular Biology, 2021 Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity.
Waqar Ahmad +3 more
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Agronomy, 2021 RNA Interference (RNAi), which works against invading nucleic acids or modulates the expression of endogenous genes, is a natural eukaryotic regulating system, and it works by noncoding smaller RNA molecules.
Mohammad Faisal +2 more
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Isolation and detection of small RNA molecules
Plant, Soil and Environment, 2007 The article describes in detail methodical procedures for isolation of small RNA molecules, their electrophoresis, northern blotting and finally detection by hybridization with radioactively labeled riboprobes.
J. Fulneček
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Primary culture and mRNA analysis of human ovarian cells
Biological Procedures Online, 2002 Established cell lines are invaluable for studying cell and molecular biological questions. A variety of human ovarian cancer (OC) cell lines exist, however, most have acquired significant genetic alterations from their cells of origin, including ...
Dunfield Lesley D. +2 more
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Polyclonal antibodies against potato spindle tuber viroid RNA
Frontiers in Bioscience-Elite, 2022 Ribonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with
Yuri Drygin +2 more
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Biological characterization of coronavirus noncanonical transcripts in vitro and in vivo
Virology Journal, 2023 Background In addition to the well-known coronavirus genomes and subgenomic mRNAs, the existence of other coronavirus RNA species, which are collectively referred to as noncanonical transcripts, has been suggested; however, their biological ...
Ching-Hung Lin +10 more
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Northern Blotting of RNA Denatured in Glyoxal Without Buffer Recirculation
BioTechniques, 1997 A rapid and easy procedure for preparing Northern blots is described. RNA is denatured with glyoxal in the presence of ethidium bromide and glycerol, then electrophoresed through agarose in a buffer that does not require recirculation.
William V. Burnett
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RNA Isolation and Northern Blot Analysis
Bio-Protocol, 2014 The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA in a sample. With northern blotting it is possible to observe particular gene expression levels during differentiation, morphogenesis, as ...
Ying Liao +5 more
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Frontiers in Microbiology, 2017 In bacteria, small non-coding RNAs (sRNAs) are critical regulators of cellular adaptation to changes in metabolism, physiology, or the external environment.
Xie Fuli +7 more
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