Results 21 to 30 of about 54,464 (302)

melRNA-seq for Expression Analysis of SINE RNAs and Other Medium-Length Non-Coding RNAs

Mobile DNA, 2021
Background Small interspersed elements (SINEs) are transcribed by RNA polymerase III (Pol III) to produce RNAs typically 100–500 nucleotides in length.
Yoshinobu Mori, Kenji Ichiyanagi
doaj   +1 more source

Methods for the Detection of D-Amino-Acid Oxidase

Biological Procedures Online, 1998
Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.
Konno Ryuichi
doaj   +1 more source

Atorvastatin action involves diminished recovery of hepatic HMG-CoA reductase activity

Journal of Lipid Research, 1998
The effects of atorvastatin on the expression of the hepatic HMG-CoA reductase and LDL receptor genes were investigated in rats. Like the other statins, atorvastatin increased the rate of degradation and presumably cycling of the hepatic LDL receptor. In
Gene C. Ness, Christopher M. Chambers, Dayami Lopez   +2 more
doaj   +1 more source

Evaluation of the Stress Effect on Expression of BEST-5 Gene in Gultured Rat Hepatocytes and Cloning of This Gene [PDF]

Journal of Kerman University of Medical Sciences, 2006
Introduction:Liver has important roles in body metabolic regulation and for this reason hepatocytes are used worldwide.Investigations showed that isolation of hepatocytes causes activation of stress related genes.The aim of this study was to study the ...
GH.H Hasanshahi, A Jafarzadeh, H Hakimi, M Rezaeian, R Vaziri nejhad, S.Z Tabatabaei, A Esmaeili, E Dikson   +7 more
doaj  

Cell type-specific over-expression of chromosome 21 genes in fibroblasts and fetal hearts with trisomy 21

BMC Medical Genetics, 2006
Background Down syndrome (DS) is caused by trisomy 21 (+21), but the aberrations in gene expression resulting from this chromosomal aneuploidy are not yet completely understood.
Zigman Warren B, Silverman Wayne, Schupf Nicole, Salas Martha, Guo Meirong, Li Chi-Ming, Husain Sameera, Warburton Dorothy, Thaker Harshwardhan, Tycko Benjamin   +9 more
doaj   +1 more source

Optimized RNA Extraction and Northern Hybridization in Streptomycetes

Biological Procedures Online, 2010
Northern blot hybridization is a useful tool for analyzing transcript patterns. To get a picture of what really occurs in vivo, it is necessary to use a protocol allowing full protection of the RNA integrity and recovery and unbiased transfer of the ...
Tagliavia Marcello, Taravella Anna, Marineo Sandra, Puglia Anna, La Farina Mario   +4 more
doaj   +3 more sources

Identification and characteristics of microRNAs from Bombyx mori

BMC Genomics, 2008
Background MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression by targeting messenger RNAs (mRNAs) and causing mRNA cleavage or translation blockage. Of the 355 Arthropod miRNAs that have been identified, only 21 are B.
Gao Xiaolian, Zhou Songping, Sheng Qing, Lv Zhengbing, Chen Jian, Chen Jianqing, Nie Zuoming, He Ping-an, Kong Lingyin, Wu Xiangfu, Jin Yongfeng, Zhang Yaozhou   +11 more
doaj   +1 more source

Polysome Profiling Analysis

Bio-Protocol, 2013
Polysome profiling is a method that allows monitoring of translation activity of mRNAs in cells and tissues. Once each polysome fractions are collected, the translation activity of each mRNA is analyzed using various molecular biology techniques such as ...
Masahiro Morita, Tommy Alain, Ivan Topisirovic, Nahum Sonenberg   +3 more
doaj   +1 more source

Near-infrared fluorescent northern blot [PDF]

RNA, 2018
Northern blot analysis detects RNA molecules immobilized on nylon membranes through hybridization with radioactive 32P-labeled DNA or RNA oligonucleotide probes. Alternatively, nonradioactive northern blot relies on chemiluminescent reactions triggered by horseradish peroxidase (HRP) conjugated probes.
Miller, Bret R., Wei, Tianqi, Fields, Christopher J., Sheng, Peike, Xie, Mingyi   +4 more
openaire   +2 more sources

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

FEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava, Anoop Saraya, Deepak Gunjan, Rinu Sharma   +3 more
wiley   +1 more source