PLoS ONE, 2017 The objective was to develop a method of HCV genome sequencing that allowed simultaneous genotyping and NS5A inhibitor resistance profiling. In order to validate the use of a unique RT-PCR for genotypes 1-5, 142 plasma samples from patients infected with
Elisabeth Andre-Garnier +12 more
doaj +1 more source
Hepatitis C virus NS5A: tales of a promiscuous protein
Journal of General Virology, 2004 The non-structural 5A (NS5A) protein of hepatitis C virus (HCV) has been the subject of intensive research over the last decade. It is generally accepted that NS5A is a pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell.
Andrew, Macdonald, Mark, Harris
openaire +2 more sources
Hepatitis C Virus Nonstructural 5A Protein Inhibits Lipopolysaccharide-Mediated Apoptosis of Hepatocytes by Decreasing Expression of Toll-Like Receptor 4 [PDF]
, 2017 Background. Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) has been shown to modulate multiple cellular processes, including apoptosis. The aim of this study was to assess the effects of HCV NS5A on apoptosis induced by Toll-like receptor (TLR ...
Arai, Makoto +12 more
core
Hepatitis C virus production requires apolipoprotein A-I and affects its association with nascent low-density lipoproteins [PDF]
, 2011 Background/aims The life cycle of hepatitis C virus (HCV) is intimately linked to the lipid metabolism of the host. In particular, HCV exploits the metabolic machinery of the lipoproteins in several steps of its life cycle such as circulation in the ...
Amicone, Laura +11 more
core +1 more source
Viruses, 2017 Hepatitis C virus (HCV) genotype (GT)3 is associated with increased risk of steatosis, development of cirrhosis and hepatocellular carcinoma. Limited data are available regarding genetic variability and use of direct-acting antiviral agents in these ...
Barbara Bartolini +9 more
doaj +1 more source
NS2 is dispensable for efficient assembly of hepatitis C virus-like particles in a bipartite trans-encapsidation system. [PDF]
, 2014 Infectious hepatitis C virus (HCV) particle production in the genotype 2a JFH-1-based cell culture system involves non-structural proteins in addition to canonical virion components.
Baumert +7 more
core +2 more sources
Hepatitis C virus NS5A inhibitors and drug resistance mutations
World Journal of Gastroenterology, 2014 Some direct-acting antiviral agents for hepatitis C virus (HCV), such as telaprevir and boceprevir have been available since 2011. It was reported that HCV NS5A is associated with interferon signaling related to HCV replication and hepatocarcinogenesis. HCV NS5A inhibitors efficiently inhibited HCV replication in vitro.
Shingo, Nakamoto +4 more
openaire +2 more sources
HCV core residues critical for infectivity are also involved in core-NS5A complex formation.
PLoS ONE, 2014 Hepatitis C virus (HCV) infection is a major cause of liver disease. The molecular machinery of HCV assembly and particle release remains obscure. A better understanding of the assembly events might reveal new potential antiviral strategies.
Katarzyna Gawlik +5 more
doaj +1 more source
Immunogenicity of variable regions of hepatitis C virus proteins: selection and modification of peptide epitopes to assess hepatitis C virus genotypes by ELISA [PDF]
, 1999 The immunogenicity of variable regions of hepatitis C virus (HCV) proteins was studied by ELISA by using 543 synthetic peptides from 120 variable regions and 90 sera from HCV-infected patients.
Berasain, C. (Carmen) +6 more
core
PLoS ONE, 2018 ObjectiveThe present study aimed to reveal the factors associated with virologic failure in sofosbuvir and ledipasvir (SOF/LDV)-treated patients, and identify baseline NS5A or NS5B resistance-associated substitutions (RASs).MethodsFour hundred ninety ...
Seiichi Mawatari +16 more
doaj +1 more source