Results 101 to 110 of about 12,817 (217)

Nonstructural protein 5A is incorporated into hepatitis C virus low-density particle through interaction with core protein and microtubules during intracellular transport.

PLoS ONE, 2014
Nonstructural protein 5A (NS5A) of hepatitis C virus (HCV) serves dual functions in viral RNA replication and virus assembly. Here, we demonstrate that HCV replication complex along with NS5A and Core protein was transported to the lipid droplet (LD ...
Chao-Kuen Lai, Vikas Saxena, Chung-Hsin Tseng, King-Song Jeng, Michinori Kohara, Michael M C Lai   +5 more
doaj   +1 more source

Genetic diversity of NS5A protein from hepatitis C virus genotype 3a and its relationship to therapy response

BMC Infectious Diseases, 2010
Background The quasispecies nature of HCV may have important implications for viral persistence, pathogenicity and resistance to antiviral agents. The variability of one of the viral proteins, NS5A, is believed to be related to the response to IFN ...
Rahal Paula, de Carvalho-Mello Isabel, Pinho João, de Queiróz Artur TL, Carareto Claudia MA, Yamasaki Lilian HT, Jardim Ana, Bittar Cíntia   +7 more
doaj   +1 more source

NS5A stability of H77C(1a) mutants.

, 2012
Amounts of NS5A were compared to Core after spread of infection to >80% of Huh7.5 cells transfected with H77C(1a) and P346A/P351A/P354A, Δ414-428 or “Δ414-428+20aa” mutants.
Thomas H. R. Carlsen (162324), Jens Bukh (162343), Troels K. H. Scheel (162316), Lotte S. Mikkelsen (162329), Judith M. Gottwein (162337), Jannick Prentoe (162323)   +5 more
core   +1 more source

Mapping of the PI4KIIIα - interaction site within NS5A domain 1.

, 2013
A: Schematic representation of expression constructs with deletions in NS5A D1 used to identify the PI4KIIIα interaction site. HCV coding sequences are indicated by blue or grey boxes, deleted sequences by black lines.
Volker Lohmann (115772), Danijela Radujkovic (411606), Alessia Ruggieri (76062), Simon Reiss (411604), Ralf Bartenschlager (32666), Ilka Rebhan (411608), Christian Harak (411605), Rahel Klein (411607), Inés Romero-Brey (113851)   +8 more
core   +1 more source

Two different NS5A dimer-interface mutations impaired both gt1a H77 and gt2a JFH1 NS5A self-interactions.

, 2018
(A) An NS5A self-interaction was determined by performing a checkmate mammalian two-hybrid assay in Huh-7 cells. Interaction between GAL4/BD (GAL4 DNA binding domain)-fused protein and VP16/AD (VP16 activation domain)-fused protein resulted in increased ...
Alyssa K. Nichols (5545664), MinKyung Yi (362029), Saravanabalaji Shanmugam (5545667), Dhanaranjani Saravanabalaji (5545670), Christoph Welsch (22852)   +4 more
core   +1 more source

Interfering with interferon: developing a reporter system to study the interaction between hepatitus C viral proteins and the interferon signalling pathway [PDF]

, 2008
PhDThe aim of the project was to investigate the mechanism by which HCV evades therapeutic IFN treatment. This involved the development of novel testing systems and their application to patient samples.
Jones, Meleri
core   +1 more source

Hepatitis C virus nonstructural protein 5A perturbs lipid metabolism by modulating AMPK/SREBP-1c signaling

Lipids in Health and Disease, 2019
Background Steatosis is an important clinical manifestation associated with chronic hepatitis C virus (HCV) infection. AMP-activated protein kinase (AMPK), a major mediator of lipid metabolism, regulates HCV-associated hepatic steatosis, but the ...
Ziyu Meng, Qiang Liu, Fujun Sun, Ling Qiao   +3 more
doaj   +1 more source

NS5A inhibitors impair NS5A-phosphatidylinositol 4-kinase III∝ complex formation and cause a decrease of phosphatidylinositol 4-phosphate and cholesterol levels in hepatitis C virus-associated membranes

, 2014
The hepatitis C virus (HCV) nonstructural (NS) protein 5A is a multifunctional protein that plays a central role in viral replication and assembly. Antiviral agents directly targeting NS5A are currently in clinical development.
P. Neddermann, S. Fenu, L. Donnici, V. Berno, M. Pagani, R. D. Francesco, V. Calabrese, S. Abrignani, V. Reghellin, F. Peri   +9 more
core   +1 more source

An NS5A single optimized method to determine genotype, subtype and resistance profiles of Hepatitis C strains.

PLoS ONE, 2017
The objective was to develop a method of HCV genome sequencing that allowed simultaneous genotyping and NS5A inhibitor resistance profiling. In order to validate the use of a unique RT-PCR for genotypes 1-5, 142 plasma samples from patients infected with
Elisabeth Andre-Garnier, Bernard Besse, Audrey Rodallec, Olivier Ribeyrol, Virginie Ferre, Caroline Luco, Laura Le Guen, Nathalie Bourgeois, Jérôme Gournay, Eric Billaud, François Raffi, Marianne Coste-Burel, Berthe-Marie Imbert-Marcille   +12 more
doaj   +1 more source

HCV core residues critical for infectivity are also involved in core-NS5A complex formation.

PLoS ONE, 2014
Hepatitis C virus (HCV) infection is a major cause of liver disease. The molecular machinery of HCV assembly and particle release remains obscure. A better understanding of the assembly events might reveal new potential antiviral strategies.
Katarzyna Gawlik, James Baugh, Udayan Chatterji, Precious J Lim, Michael D Bobardt, Philippe A Gallay   +5 more
doaj   +1 more source