Results 81 to 90 of about 12,817 (217)

Dominant negative effect of wild-type NS5A on NS5A-adapted subgenomic hepatitis C virus RNA replicon

Journal of General Virology, 2004
An efficient model is currently used to study hepatitis C virus (HCV) replication in cell culture. It involves transfection in Huh7, a hepatoma-derived cell line, of an antibiotic (neomycin) selectable HCV subgenomic replicon encoding the non-structural (NS) proteins from NS3 to NS5B.
Rita, Graziani, Giacomo, Paonessa
openaire   +2 more sources

Expression of NS5A in transgenic mice.

, 2017
(A) Expression levels of NS5A protein in liver tissues of NS5A-transgenic (Tg) and non-transgenic (NT) mice were determined by immunoblotting with mouse anti-NS5A antibody.
Kenichiro Enooku (465621), Kazuya Okushin (3687322), Tetsuro Suzuki (180340), Yoshiharu Matsuura (357989), Takeya Tsutsumi (699707), Hidetaka Fujinaga (3687325), Hideki Aizaki (139942), Kazuhiko Koike (135022), Kyoji Moriya (478623), Hiroshi Yotsuyanagi (699706)   +9 more
core   +1 more source

DEB025 (Alisporivir) inhibits hepatitis C virus replication by preventing a cyclophilin A induced cis-trans isomerisation in domain II of NS5A. [PDF]

PLoS ONE, 2010
DEB025/Debio 025 (Alisporivir) is a cyclophilin (Cyp)-binding molecule with potent anti-hepatitis C virus (HCV) activity both in vitro and in vivo. It is currently being evaluated in phase II clinical trials.
Lotte Coelmont, Xavier Hanoulle, Udayan Chatterji, Carola Berger, Joke Snoeck, Michael Bobardt, Precious Lim, Inge Vliegen, Jan Paeshuyse, Grégoire Vuagniaux, Anne-Mieke Vandamme, Anne-Mieke Vandamme, Ralf Bartenschlager, Philippe Gallay, Guy Lippens, Johan Neyts   +15 more
doaj   +1 more source

Hepatitis C Virus Nonstructural Protein 5A Inhibits MG132-Induced Apoptosis of Hepatocytes in Line with NF-κB-Nuclear Translocation.

PLoS ONE, 2015
BackgroundHepatitis C virus (HCV) infection is one of the major causes of cirrhosis and hepatocellular carcinoma. HCV nonstructural protein 5A (NS5A) is an attractive antiviral target and plays an important role in HCV replication as well as ...
Xia Jiang, Tatsuo Kanda, Shuang Wu, Shingo Nakamoto, Masato Nakamura, Reina Sasaki, Yuki Haga, Takaji Wakita, Hiroshi Shirasawa, Osamu Yokosuka   +9 more
doaj   +1 more source

Efficacy and Safety of 12‐Week Coblopasvir/Sofosbuvir Regimen for Hepatitis C in Northwest China: A Multi‐Center Prospective Study

Journal of Medical Virology, Volume 98, Issue 5, May 2026.
ABSTRACT To assess the sustained virologic response (SVR12) and safety profile of 12‐week coblopasvir/sofosbuvir (COB/SOF) therapy in hepatitis C virus (HCV)‐infected patients across genotypes in Northwest China. This prospective cohort study (2023–2024) enrolled HCV patients from 12 clinical centers in Northwest China, adhering to the China Hepatitis ...
Lu Yan, Maimaitijiang Wubuliaishan, Xiaoni Kou, Maimaitiaili Tuerxun, Mingbo Yang, Fanpu Ji, Yingli He, Jiuping Wang, Longdong Zhu, Xiaohong Gao, Fuchun Jing, Xiangchun Ding, Hong Du, Ye Zhang, Hong Jiang   +14 more
wiley   +1 more source

NS5A inhibitors in the treatment of hepatitis C

Journal of Hepatology, 2013
Hepatitis C virus infection is a major health problem worldwide and no vaccine has yet been developed against this virus. In addition, currently approved pharmacotherapies achieve suboptimal cure rates and have side effects that result in non-compliance and premature treatment discontinuation.
openaire   +2 more sources

Interaction between Nonstructural Proteins NS4B and NS5A Is Essential for Proper NS5A Localization and Hepatitis C Virus RNA Replication [PDF]

Journal of Virology, 2016
ABSTRACT The hepatitis C virus NS5A protein is tethered to cellular membranes via an amphipathic amino-terminal helix that is inserted in-plane into the outer endoplasmic reticulum (ER)-derived membrane leaflet. The charged face of the helix faces the cytoplasm and may contribute to interactions involved in replicase assembly and function ...
Avik, Biswas, Jason, Treadaway, Timothy L, Tellinghuisen   +2 more
openaire   +2 more sources

Identification of cellular factors interacting with HCV NS5A.

, 2013
(A) Purification of cellular proteins interacting with NS5A. Huh7.5 cells were electroporated with either pNTAP empty vector or pNTAP-NS5A plasmid. At 3 days after electroporation, cells were harvested and then TAP-tagged NS5A and its associated protein ...
Yun-Sook Lim (157976), Soon B. Hwang (157980), Jong-Wook Kim (430540), Seung-Ae Yim (430539)   +3 more
core   +1 more source

Hepatitis C virus nonstructural protein 5A inhibits thapsigargin-induced apoptosis.

PLoS ONE, 2014
BackgroundWe previously reported that the hepatitis C virus (HCV) nonstructural protein 5A (NS5A) down-regulates TLR4 signaling and lipopolysaccharide-induced apoptosis of hepatocytes. There have been several reports regarding the association between HCV
Xia Jiang, Tatsuo Kanda, Shuang Wu, Shingo Nakamoto, Takaji Wakita, Hiroshi Shirasawa, Osamu Yokosuka   +6 more
doaj   +1 more source

Quantitative Analysis of Hepatitis C NS5A Viral Protein Dynamics on the ER Surface

Viruses, 2018
Exploring biophysical properties of virus-encoded components and their requirement for virus replication is an exciting new area of interdisciplinary virological research.
Markus M. Knodel, Arne Nägel, Sebastian Reiter, Andreas Vogel, Paul Targett-Adams, John McLauchlan, Eva Herrmann, Gabriel Wittum   +7 more
doaj   +1 more source