Results 81 to 90 of about 9,676 (179)

Genetic diversity of NS5A protein from hepatitis C virus genotype 3a and its relationship to therapy response

BMC Infectious Diseases, 2010
Background The quasispecies nature of HCV may have important implications for viral persistence, pathogenicity and resistance to antiviral agents. The variability of one of the viral proteins, NS5A, is believed to be related to the response to IFN ...
Rahal Paula, de Carvalho-Mello Isabel, Pinho João, de Queiróz Artur TL, Carareto Claudia MA, Yamasaki Lilian HT, Jardim Ana, Bittar Cíntia   +7 more
doaj   +1 more source

Hepatitis C virus nonstructural protein 5A perturbs lipid metabolism by modulating AMPK/SREBP-1c signaling

Lipids in Health and Disease, 2019
Background Steatosis is an important clinical manifestation associated with chronic hepatitis C virus (HCV) infection. AMP-activated protein kinase (AMPK), a major mediator of lipid metabolism, regulates HCV-associated hepatic steatosis, but the ...
Ziyu Meng, Qiang Liu, Fujun Sun, Ling Qiao   +3 more
doaj   +1 more source

Hepatitis C virus NS5A: tales of a promiscuous protein

Journal of General Virology, 2004
The non-structural 5A (NS5A) protein of hepatitis C virus (HCV) has been the subject of intensive research over the last decade. It is generally accepted that NS5A is a pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell.
Andrew, Macdonald, Mark, Harris
openaire   +2 more sources

An NS5A single optimized method to determine genotype, subtype and resistance profiles of Hepatitis C strains.

PLoS ONE, 2017
The objective was to develop a method of HCV genome sequencing that allowed simultaneous genotyping and NS5A inhibitor resistance profiling. In order to validate the use of a unique RT-PCR for genotypes 1-5, 142 plasma samples from patients infected with
Elisabeth Andre-Garnier, Bernard Besse, Audrey Rodallec, Olivier Ribeyrol, Virginie Ferre, Caroline Luco, Laura Le Guen, Nathalie Bourgeois, Jérôme Gournay, Eric Billaud, François Raffi, Marianne Coste-Burel, Berthe-Marie Imbert-Marcille   +12 more
doaj   +1 more source

HCV RNA traffic and association with NS5A in living cells

Virology, 2016
The spatiotemporal dynamics of Hepatitis C Virus (HCV) RNA localisation are poorly understood. To address this we engineered HCV genomes harbouring MS2 bacteriophage RNA stem-loops within the 3'-untranslated region to allow tracking of HCV RNA via specific interaction with a MS2-Coat-mCherry fusion protein.
Fiches, GN, Eyre, NS, Aloia, AL, Van Der Hoek, K, Betz-Stablein, B, Luciani, F, Chopra, A, Beard, MR, Maher, Lisa   +8 more
openaire   +5 more sources

Characterization of Naturally Occurring NS5A and NS5B Polymorphisms in Patients Infected with HCV Genotype 3a Treated with Direct-Acting Antiviral Agents

Viruses, 2017
Hepatitis C virus (HCV) genotype (GT)3 is associated with increased risk of steatosis, development of cirrhosis and hepatocellular carcinoma. Limited data are available regarding genetic variability and use of direct-acting antiviral agents in these ...
Barbara Bartolini, Emanuela Giombini, Chiara Taibi, Raffaella Lionetti, Marzia Montalbano, Ubaldo Visco-Comandini, Gianpiero D’Offizi, Maria Rosaria Capobianchi, Fiona McPhee, Anna Rosa Garbuglia   +9 more
doaj   +1 more source

HCV core residues critical for infectivity are also involved in core-NS5A complex formation.

PLoS ONE, 2014
Hepatitis C virus (HCV) infection is a major cause of liver disease. The molecular machinery of HCV assembly and particle release remains obscure. A better understanding of the assembly events might reveal new potential antiviral strategies.
Katarzyna Gawlik, James Baugh, Udayan Chatterji, Precious J Lim, Michael D Bobardt, Philippe A Gallay   +5 more
doaj   +1 more source

The co-existence of NS5A and NS5B resistance-associated substitutions is associated with virologic failure in Hepatitis C Virus genotype 1 patients treated with sofosbuvir and ledipasvir.

PLoS ONE, 2018
ObjectiveThe present study aimed to reveal the factors associated with virologic failure in sofosbuvir and ledipasvir (SOF/LDV)-treated patients, and identify baseline NS5A or NS5B resistance-associated substitutions (RASs).MethodsFour hundred ninety ...
Seiichi Mawatari, Kohei Oda, Kazuaki Tabu, Sho Ijuin, Kotaro Kumagai, Kunio Fujisaki, Masafumi Hashiguchi, Yukiko Inada, Hirofumi Uto, Yasunari Hiramine, Takeshi Kure, Takeshi Hori, Oki Taniyama, Ai Kasai, Tsutomu Tamai, Akihiro Moriuchi, Akio Ido   +16 more
doaj   +1 more source

Hepatitis C virus NS5A and core protein induce fibrosis-related genes regulation on Huh7 cells through activation of LX2 cells

Annals of Hepatology
Introduction and Objectives: Liver fibrosis remains a complication derived from a chronic Hepatitis C Virus (HCV) infection even when it is resolved, and no liver antifibrotic drug has been approved.
Tania G. Heredia-Torres, Veronica Alvarado-Martínez, Ana R. Rincón-Sánchez, Sonia A. Lozano-Sepúlveda, Kame A. Galán-Huerta, Daniel Arellanos-Soto, Ana M. Rivas-Estilla   +6 more
doaj   +1 more source

Should NS5A inhibitors serve as the scaffold for all-oral anti-HCV combination therapies?

Hepatic Medicine: Evidence and Research, 2015
Sujit V Janardhan, Nancy S Reau Center for Liver Diseases, Section of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Chicago, Chicago, IL, USA Abstract: Chronic hepatitis C virus (HCV) infection represents a global ...
Janardhan SV, Reau NS
doaj