Results 21 to 30 of about 38,049 (143)

The worldwide search for the new mutations in the RNA-directed RNA Polymerase domain of SARS-CoV-2

Macedonian Veterinary Review, 2021
Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is an RNA virus, responsible for the current pandemic outbreak. In total, 200 genomes of the SARS‐CoV‐2 strains from four host organisms have been analyzed.
Siarhei A. Dabravolski, Yury K. Kavalionak   +1 more
doaj   +1 more source

SARS-CoV-2 nsp12 attenuates type I interferon production by inhibiting IRF3 nuclear translocation [PDF]

Cellular & Molecular Immunology, 2021
AbstractSARS-CoV-2 is the pathogenic agent of COVID-19, which has evolved into a global pandemic. Compared with some other respiratory RNA viruses, SARS-CoV-2 is a poor inducer of type I interferon (IFN). Here, we report that SARS-CoV-2 nsp12, the viral RNA-dependent RNA polymerase (RdRp), suppresses host antiviral responses.
Wenjing Wang, Zhuo Zhou, Xia Xiao, Zhongqin Tian, Xuan Dong, Conghui Wang, Li Li, Lili Ren, Xiaobo Lei, Xiang Zou, Jianwei Wang   +10 more
openaire   +2 more sources

SARS-CoV-2 variants with NSP12 P323L/G671S mutations display enhanced virus replication in ferret upper airways and higher transmissibility

Cell Reports, 2023
Summary: With the emergence of multiple predominant SARS-CoV-2 variants, it becomes important to have a comprehensive assessment of their viral fitness and transmissibility.
Se-Mi Kim, Eun-Ha Kim, Mark Anthony B. Casel, Young-Il Kim, Rong Sun, Mi-Jeong Kwak, Ji-Seung Yoo, Mina Yu, Kwang-Min Yu, Seung-Gyu Jang, Rare Rollon, Jeong Ho Choi, Juryeon Gil, Kiyoung Eun, Hyunggee Kim, Armin Ensser, Jungwon Hwang, Min-Suk Song, Myung Hee Kim, Jae U. Jung, Young Ki Choi   +20 more
doaj   +1 more source

Inspection on the Mechanism of SARS-CoV-2 Inhibition by Penciclovir: A Molecular Dynamic Study

Molecules, 2022
In recent years, humanity has had to face a critical pandemic due to SARS-CoV-2. In the rapid search for effective drugs against this RNA-positive virus, the repurposing of already existing nucleotide/nucleoside analogs able to stop RNA replication by ...
Micaela Giannetti, Claudia Mazzuca, Giorgio Ripani, Antonio Palleschi   +3 more
doaj   +1 more source

The SARS-CoV nsp12 Polymerase Active Site Is Tuned for Large-Genome Replication

Journal of Virology, 2022
Replicating large genomes represents a challenge for RNA viruses because fast RNA synthesis is needed to escape innate immunity defenses, but faster polymerases are inherently low-fidelity enzymes. Nonetheless, the coronaviruses replicate their ≈30-kb genomes using the core polymerase structure and mechanism common to all positive-strand RNA viruses.
Campagnola, Grace, Govindarajan, Vishnu, Pelletier, Annelise, Canard, Bruno, Peersen, Olve   +4 more
openaire   +3 more sources

Evidence for broad cross-reactivity of the SARS-CoV-2 NSP12-directed CD4+ T-cell response with pre-primed responses directed against common cold coronaviruses

Frontiers in Immunology, 2023
IntroductionThe nonstructural protein 12 (NSP12) of the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) has a high sequence identity with common cold coronaviruses (CCC).MethodsHere, we comprehensively assessed the breadth and ...
Tim Westphal, Tim Westphal, Maria Mader, Hendrik Karsten, Leon Cords, Maximilian Knapp, Sophia Schulte, Lennart Hermanussen, Sven Peine, Vanessa Ditt, Alba Grifoni, Marylyn Martina Addo, Marylyn Martina Addo, Marylyn Martina Addo, Marylyn Martina Addo, Samuel Huber, Alessandro Sette, Marc Lütgehetmann, Marc Lütgehetmann, Sven Pischke, Sven Pischke, William W. Kwok, John Sidney, Julian Schulze zur Wiesch, Julian Schulze zur Wiesch   +24 more
doaj   +1 more source

Andrographolide binds to spike glycoprotein and RNA-dependent RNA polymerase (NSP12) of SARS-CoV-2 by in silico approach: a probable molecule in the development of anti-coronaviral drug

Journal of Genetic Engineering and Biotechnology, 2021
The SARS-CoV-2 belongs to Coronaviridae family infects host cells by the interaction of its spike glycoprotein and angiotensin-converting enzyme 2 (ACE 2) of host cells. Upon entry, the virus uses its RNA dependent RNA polymerase (NSP12) for transcribing
Lokanathan Srikanth, Potukuchi Venkata Gurunadha Krishna Sarma   +1 more
doaj   +1 more source

The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent [PDF]

Nucleic Acids Research, 2009
An RNA-dependent RNA polymerase (RdRp) is the central catalytic subunit of the RNA-synthesizing machinery of all positive-strand RNA viruses. Usually, RdRp domains are readily identifiable by comparative sequence analysis, but biochemical confirmation and characterization can be hampered by intrinsic protein properties and technical complications.
Velthuis, A.J.W.T., Arnold, J.J., Cameron, C.E., Worm, S.H.E. van den, Snijder, E.J.   +4 more
openaire   +4 more sources

Coronavirus RNA-dependent RNA polymerase interacts with the p50 regulatory subunit of host DNA polymerase delta and plays a synergistic role with RNA helicase in the induction of DNA damage response and cell cycle arrest in the S phase

Emerging Microbes and Infections, 2023
Disruption of the cell cycle is a common strategy shared by many viruses to create a conducible cellular microenvironment for their efficient replication. We have previously shown that infection of cells with gammacoronavirus infectious bronchitis virus (
Li Quan, Xinxin Sun, Linghui Xu, Rui Ai Chen, Ding Xiang Liu   +4 more
doaj   +1 more source

Transient SARS-CoV-2 RNA-Dependent RNA Polymerase Mutations after Remdesivir Treatment for Chronic COVID-19 in Two Transplant Recipients: Case Report and Intra-Host Viral Genomic Investigation

Microorganisms, 2023
Remdesivir is the first FDA-approved drug for treating severe SARS-CoV-2 infection and targets RNA-dependent RNA polymerase (RdRp) that is required for viral replication.
Shangxin Yang, Ashrit Multani, Jacob M. Garrigues, Michael S. Oh, Peera Hemarajata, Taylor Burleson, Nicole M. Green, Caspian Oliai, Pryce T. Gaynor, Omer E. Beaird, Drew J. Winston, Christopher S. Seet, Joanna M. Schaenman   +12 more
doaj   +1 more source