Cellular eEF1G Inhibits Porcine Deltacoronavirus Replication by Binding Nsp12 and Disrupting Its Interaction With Viral Genomic RNA [PDF]
VirusesPorcine deltacoronavirus (PDCoV) is an emerging pathogen that causes severe, often fatal, diarrhea in suckling piglets and has zoonotic potential. Its nonstructural protein 12 (Nsp12), functioning as the RNA-dependent RNA polymerase (RdRp), is a central ...
Yongning Zhang, Xinna Ge, Jun Han
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Serine ubiquitination of the SARS-CoV-2 RdRp by the non-canonical E3 ligase MYCBP2 [PDF]
mSpherePost-translational modifications, including ubiquitination, have emerged as important regulators of viral infection and the host innate immune response.
RuthMabel Boytz +6 more
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Insights into SARS-CoV-2 replication control via targeting the host hijacking function of NSP12 [PDF]
Acta Pharmaceutica Sinica BNonstructural protein 12 (NSP12), the RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2, serves as the catalytic core of the viral replication–transcription complex and is pivotal for viral RNA synthesis.
Shirui Fan +11 more
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Adaptive mutations at lysine residues of PRRSV-2 nsp12 enable evasion of host proteasomal degradation to promote subgenomic RNA synthesis [PDF]
Journal of VirologyUbiquitin signaling in viral infections can either enhance immune responses and degrade viral proteins or be exploited by viruses to target host antiviral factors.
Yongjie Chen +5 more
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Cell Reports, 2023 Summary: With the emergence of multiple predominant SARS-CoV-2 variants, it becomes important to have a comprehensive assessment of their viral fitness and transmissibility.
Young Ki Choi
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GSK3 maintains NSP12 stability to promote bat-origin swine acute diarrhea syndrome coronavirus infection [PDF]
Journal of VirologyBat-origin swine acute diarrhea syndrome coronavirus (SADS-CoV) causes diarrhea and death in piglets and also poses a cross-species transmission risk due to its broad host cell tropism.
Maowen Sun +8 more
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Frontiers in Immunology, 2023 IntroductionThe nonstructural protein 12 (NSP12) of the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) has a high sequence identity with common cold coronaviruses (CCC).MethodsHere, we comprehensively assessed the breadth and ...
Maria Mader, Vanessa Ditt
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The Nsp12-Coding Region of Type 2 PRRSV Is Required for Viral Subgenomic mRNA Synthesis
Emerging Microbes and Infections, 2019 As one of many nonstructural proteins of porcine reproductive and respiratory syndrome virus (PRRSV), nonstructural protein 12 (Nsp12) has received relatively little attention, and its role in virus replication, if any, is essentially unknown.
Yan-Dong Tang +2 more
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Virologic insights from the phase 3 REDPINE trial: remdesivir in renally impaired and immunocompromised patients with COVID-19 [PDF]
Antimicrobial Agents and ChemotherapyRemdesivir inhibits the SARS-CoV-2 RNA-dependent polymerase (Nsp12). Here, we present virology analyses from the Phase 3 REDPINE trial in participants with impaired kidney function hospitalized for COVID-19 and treated with remdesivir or placebo for 5 ...
Lauren Rodriguez +15 more
doaj +2 more sources
Redefining NSP12 Activity in SARS-CoV-2 and Its Regulation by NSP8 and NSP7
Molecular Therapy - Nucleic AcidsRdRp is a critical component of an RNA virus life cycle. Among coronaviruses, NSP12, along with one copy of NSP7 and two copies of NSP8, forms the RdRp holoenzyme and exhibits polymerase activity.
Krishna K Inampudi
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