Results 1 to 10 of about 38,894 (153)
The Nsp12-coding region of type 2 PRRSV is required for viral subgenomic mRNA synthesis [PDF]
Emerging Microbes and Infections, 2019 As one of many nonstructural proteins of porcine reproductive and respiratory syndrome virus (PRRSV), nonstructural protein 12 (Nsp12) has received relatively little attention, and its role in virus replication, if any, is essentially unknown.
Tong-Yun Wang +2 more
exaly +6 more sources
The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent [PDF]
Nucleic Acids Research, 2010 An RNA-dependent RNA polymerase (RdRp) is the central catalytic subunit of the RNA-synthesizing machinery of all positive-strand RNA viruses. Usually, RdRp domains are readily identifiable by comparative sequence analysis, but biochemical confirmation ...
Aartjan J W Te Velthuis +2 more
exaly +8 more sources
Journal of Biomolecular Structure and Dynamics, 2023 SARS-COV-2 is responsible for the COVID-19 pandemic, which has infected more than 767 million people worldwide including about 7 million deaths till 5 June 2023.
N. R. Jena (2329891) +1 more
core +3 more sources
SARS-CoV-2 NSP12 associates with TRiC and the P323L substitution acts as a host adaption. [PDF]
Journal of Virology, 2023 ImportanceSARS-CoV-2 has caused a worldwide health and economic crisis. During the course of the pandemic, genetic changes occurred in the virus, which have resulted in new properties of the virus-particularly around gains in transmission and the ability
Armstrong, Stuart +8 more
core +3 more sources
SARS-CoV-2 NSP12 Protein Is Not an Interferon-β Antagonist
Journal of Virology, 2021 Previous studies investigated the interaction between SARS-CoV-2 viral proteins and interferon signaling and proposed that several SARS-CoV-2 viral proteins, including NSP12, could suppress IFN-β activation. However, most of these results were generated from IFN-β promoter luciferase reporter assay and have not been validated functionally.
Kaitao Zhao +2 more
exaly +4 more sources
Transient and stabilized complexes of Nsp7, Nsp8, and Nsp12 in SARS-CoV-2 replication [PDF]
Biophysical Journal, 2021 The replication transcription complex (RTC) from the virus SARS-CoV-2 is responsible for recognizing and processing RNA for two principal purposes. The RTC copies viral RNA for propagation into new virus and for ribosomal transcription of viral proteins.
Zhang, Qiu +23 more
core +8 more sources
Preparation of SARS-CoV-2 Polymerase Nsp12 and Optimization of Expression Conditions
Sustainability in Environment, 2023 he core component of transcription and replication of SARS-CoV-2 is Nsp12, a non-structural protein that function as an RNA-dependent RNA polymerase. Nsp12 is a key drug target for the development of anti-coronavirus drugs.
Liu, Yifei
core +2 more sources
Cell Reports, 2023 With the emergence of multiple predominant SARS-CoV-2 variants, it becomes important to have a comprehensive assessment of their viral fitness and transmissibility.
Young Ki Choi
exaly +2 more sources
SARS-CoV-2 nsp12 attenuates type I interferon production by inhibiting IRF3 nuclear translocation [PDF]
Cellular and Molecular Immunology, 2021 AbstractSARS-CoV-2 is the pathogenic agent of COVID-19, which has evolved into a global pandemic. Compared with some other respiratory RNA viruses, SARS-CoV-2 is a poor inducer of type I interferon (IFN). Here, we report that SARS-CoV-2 nsp12, the viral RNA-dependent RNA polymerase (RdRp), suppresses host antiviral responses.
Zhuo Zhou, Xiaojing Dong, Xiaobo Lei
exaly +3 more sources
Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12 [PDF]
Molecular Therapy, 2021 Lung inflammation is a hallmark of coronavirus disease 2019 (COVID-19). In this study, we show that mice develop inflamed lung tissue after being administered exosomes released from the lung epithelial cells exposed to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Nsp12 and Nsp13 (exosomesNsp12Nsp13).
Xiang Yang Zhang +2 more
exaly +3 more sources