Results 11 to 20 of about 40,674 (185)

Structure of the SARS-CoV nsp12 Polymerase Bound to nsp7 and nsp8 Co-Factors [PDF]

Nature Communications, 2019
The pathogenic human coronaviruses SARS- and MERS-CoV can cause severe respiratory disease. Here the authors present the 3.1Å cryo-EM structure of the SARS-CoV RNA polymerase nsp12 bound to its essential co-factors nsp7 and nsp8, which is of interest for
Andrew B Ward, Robert N Kirchdoerfer
exaly   +4 more sources

SARS-CoV-2 Remdesivir Exposure Leads to Different Evolutionary Pathways That Converge in Moderate Levels of Drug Resistance [PDF]

Viruses
Various SARS-CoV-2 remdesivir resistance-associated substitutions (RAS) have been reported, but a comprehensive comparison of their resistance levels is lacking.
Carlota Fernandez-Antunez, Line A. Ryberg, Kuan Wang, Long V. Pham, Lotte S. Mikkelsen, Ulrik Fahnøe, Katrine T. Hartmann, Henrik E. Jensen, Kenn Holmbeck, Jens Bukh, Santseharay Ramirez   +10 more
doaj   +3 more sources

Peptide inhibitors derived from the nsp7 and nsp8 cofactors of nsp12 targeting different substrate binding sites of nsp12 of the SARS-CoV-2

Journal of Biomolecular Structure and Dynamics, 2023
SARS-COV-2 is responsible for the COVID-19 pandemic, which has infected more than 767 million people worldwide including about 7 million deaths till 5 June 2023. Despite the emergency use of certain vaccines, deaths due to COVID-19 have not yet stopped completed. Therefore, it is imperative to design and develop drugs that can be used to treat patients
N. R. Jena, Suyash Pant
openaire   +3 more sources

Development of novel monoclonal antibodies against nsp12 of SARS-CoV-2

Virology Journal, 2022
A novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused a global pandemic of coronavirus disease 19. Coronaviruses, including SARS-CoV-2, use RNA-dependent RNA polymerase (RdRP) for viral replication and ...
Mitsuhiro Machitani, Junko Takei, Mika K. Kaneko, Saori Ueki, Hirofumi Ohashi, Koichi Watashi, Yukinari Kato, Kenkichi Masutomi   +7 more
doaj   +3 more sources

Preparation of SARS-CoV-2 Polymerase Nsp12 and Optimization of Expression Conditions [PDF]

Sustainability in Environment, 2023
he core component of transcription and replication of SARS-CoV-2 is Nsp12, a non-structural protein that function as an RNA-dependent RNA polymerase. Nsp12 is a key drug target for the development of anti-coronavirus drugs. The purpose of this study was to explore and optimize the expression conditions of Nsp12 of SARS-CoV-2 with the aim of ...
Liu, Yifei, Yifei Liu
openaire   +2 more sources

SARS-CoV-2 NSP12 associates with TRiC and the P323L substitution acts as a host adaption [PDF]

Journal of Virology, 2023
ABSTRACT SARS-CoV-2 emerged in the human population in late 2019, and human-to-human transmission has dominated the evolutionary landscape and driven the selection of different lineages. The first major change that resulted in increased transmission was the D614G substitution in the spike protein.
Muhannad Alruwaili, Stuart Armstrong, Tessa Prince, Maximillian Erdmann, David A. Matthews, Lisa Luu, Andrew Davidson, Waleed Aljabr, Julian A. Hiscox   +8 more
openaire   +3 more sources

The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent [PDF]

Nucleic Acids Research, 2009
An RNA-dependent RNA polymerase (RdRp) is the central catalytic subunit of the RNA-synthesizing machinery of all positive-strand RNA viruses. Usually, RdRp domains are readily identifiable by comparative sequence analysis, but biochemical confirmation and characterization can be hampered by intrinsic protein properties and technical complications.
Velthuis, A.J.W.T., Arnold, J.J., Cameron, C.E., Worm, S.H.E. van den, Snijder, E.J.   +4 more
openaire   +6 more sources

Investigation of protein-protein interactions and hotspot region on the NSP7-NSP8 binding site in NSP12 of SARS-CoV-2 [PDF]

Frontiers in Molecular Biosciences
Background: The RNA-dependent RNA polymerase (RdRp) complex, essential in viral transcription and replication, is a key target for antiviral therapeutics.
José Xavier Lima Neto, Katyanna Sales Bezerra, Emmanuel Duarte Barbosa, Roniel Lima Araujo, Douglas Soares Galvão, Marcelo Leite Lyra, Jonas Ivan Nobre Oliveira, Shopnil Akash, Yousef A. Bin Jardan, Hiba-Allah Nafidi, Mohammed Bourhia, Umberto Laino Fulco   +11 more
doaj   +2 more sources

Andrographolide binds to spike glycoprotein and RNA-dependent RNA polymerase (NSP12) of SARS-CoV-2 by in silico approach: a probable molecule in the development of anti-coronaviral drug

Journal of Genetic Engineering and Biotechnology, 2021
The SARS-CoV-2 belongs to Coronaviridae family infects host cells by the interaction of its spike glycoprotein and angiotensin-converting enzyme 2 (ACE 2) of host cells. Upon entry, the virus uses its RNA dependent RNA polymerase (NSP12) for transcribing
Lokanathan Srikanth, Potukuchi Venkata Gurunadha Krishna Sarma   +1 more
doaj   +2 more sources

Transient SARS-CoV-2 RNA-Dependent RNA Polymerase Mutations after Remdesivir Treatment for Chronic COVID-19 in Two Transplant Recipients: Case Report and Intra-Host Viral Genomic Investigation [PDF]

Microorganisms, 2023
Remdesivir is the first FDA-approved drug for treating severe SARS-CoV-2 infection and targets RNA-dependent RNA polymerase (RdRp) that is required for viral replication.
Shangxin Yang, Ashrit Multani, Jacob M. Garrigues, Michael S. Oh, Peera Hemarajata, Taylor Burleson, Nicole M. Green, Caspian Oliai, Pryce T. Gaynor, Omer E. Beaird, Drew J. Winston, Christopher S. Seet, Joanna M. Schaenman   +12 more
doaj   +2 more sources