Results 31 to 40 of about 40,674 (185)

Identification of the SARS-CoV-2 genome packaging signal in the nsp12-coding region. [PDF]

Nat Commun
Selective genome packaging is a critical step for RNA viruses, which must distinguish genomic RNA from other abundant transcripts. For SARS-CoV-2, the cis-acting packaging signal is thought to be recognized by the nucleocapsid (N) protein, but its identity and mechanistic basis for selective recognition remain undefined.
Park Y, Lim J, Cho H, Son A, Jeong M, Lee YS, Kim VN.   +6 more
europepmc   +4 more sources

The SARS-CoV nsp12 Polymerase Active Site Is Tuned for Large-Genome Replication

Journal of Virology, 2022
Replicating large genomes represents a challenge for RNA viruses because fast RNA synthesis is needed to escape innate immunity defenses, but faster polymerases are inherently low-fidelity enzymes. Nonetheless, the coronaviruses replicate their ≈30-kb genomes using the core polymerase structure and mechanism common to all positive-strand RNA viruses.
Campagnola, Grace, Govindarajan, Vishnu, Pelletier, Annelise, Canard, Bruno, Peersen, Olve   +4 more
openaire   +3 more sources

Digging for the Discovery of SARS-CoV-2 nsp12 Inhibitors: A Pharmacophore-Based and Molecular Dynamics Simulation Study [PDF]

Future Virology, 2021
Abstract Background: The severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) is a grave global threat causing Coronavirus Disease 2019 (COVID-19). The therapeutics are urgently needed to cure patients severely infected with COVID-19. The aim of the study was to investigate for potential candidates of nsp12 inhibitors by searching for ...
Fatemeh Sana Askari, Mohsen Ebrahimi, Jabbar Parhiz, Mina Hassanpour, Alireza Mohebbi, Abbas Mirshafiey   +5 more
openaire   +2 more sources

RNA binding sites in NSP12 protein.

, 2023
(A) Surface notation for NSP12 protein and van der Waals notation for bound RNA. (B and C) The RNA binding space is exposed by using only the protein surface notation and cartoon notation. (PPTX)
Shiho Torii (12770667), Yong Dam Jeong (14846302), Kei Sato (493447), Rigel Suzuki (12770673), Kwang Su Kim (1960972), Jumpei Ito (761675), Hiroyuki Asakura (1617940), Teppei Shimamura (133856), Takasuke Fukuhara (673475), Yasuhisa Fujita (10344689), Shoya Iwanami (3945266), Kazuhisa Yoshimura (322429), Kenji Sadamasu (7438220), Jun Koseki (738263), Yoshiharu Matsuura (357989), Shingo Iwami (266092), Mami Nagashima (14846305)   +16 more
core   +2 more sources

Inspection on the Mechanism of SARS-CoV-2 Inhibition by Penciclovir: A Molecular Dynamic Study

Molecules, 2022
In recent years, humanity has had to face a critical pandemic due to SARS-CoV-2. In the rapid search for effective drugs against this RNA-positive virus, the repurposing of already existing nucleotide/nucleoside analogs able to stop RNA replication by ...
Micaela Giannetti, Claudia Mazzuca, Giorgio Ripani, Antonio Palleschi   +3 more
doaj   +1 more source

Coronavirus RNA-dependent RNA polymerase interacts with the p50 regulatory subunit of host DNA polymerase delta and plays a synergistic role with RNA helicase in the induction of DNA damage response and cell cycle arrest in the S phase

Emerging Microbes and Infections, 2023
Disruption of the cell cycle is a common strategy shared by many viruses to create a conducible cellular microenvironment for their efficient replication. We have previously shown that infection of cells with gammacoronavirus infectious bronchitis virus (
Li Quan, Xinxin Sun, Linghui Xu, Rui Ai Chen, Ding Xiang Liu   +4 more
doaj   +1 more source

Conservation of NSP12 E802 and NSP6 I168 in coronaviruses.

, 2021
Excel file with the frequency of NSP12 E802 (A) and frequency of NSP6 I168 (B). (XLSX)
Andres Merits (191446), Gauthier Lieber (10196207), Meredith E. Stewart (11450472), Oscar A. MacLean (10297450), Alain Kohl (252931), Sam J. Wilson (9344990), Brian J. Willett (11450469), Rute Maria Pinto (10196222), Arthur Wickenhagen (9344984), Daniel Mair (5849150), Agnieszka M. Szemiel (10196243), Wilhelm Furnon (10196183), Nicolas M. Suarez (11450466), Matthew L. Turnbull (10196156), Massimo Palmarini (81656), Ana da Silva Filipe (5849153), Arvind H. Patel (10196255), Emma C. Thomson (10196249), Richard J. Orton (7832015), Sainan Wang (713604)   +19 more
core   +1 more source

The P323L substitution in the SARS-CoV-2 polymerase (NSP12) confers a selective advantage during infection

Genome Biology, 2023
Abstract Background The mutational landscape of SARS-CoV-2 varies at the dominant viral genome sequence and minor genomic variant population. During the COVID-19 pandemic, an early substitution in the genome was the D614G change in the spike protein, associated with an increase in transmissibility. Genomes with D614G are
Goldswain, H., Dong, X., Penrice-Randal, R., Alruwaili, M., Shawli, G.T., Prince, T., Williamson, M.K., Raghwani, J., Randle, N., Jones, B., Donovan-Banfield, I., Salguero, F.J., Tree, J.A., Hall, Y., Hartley, C., Erdmann, M., Bazire, J., Jearanaiwitayakul, T., Semple, M.G., Openshaw, P.J.M., Baillie, J.K., Baillie, J.K., Semple, M.G., Openshaw, P.J.M., Carson, G., Alex, B., Andrikopoulos, P., Bach, B., Barclay, W.S., Bogaert, D., Chand, M., Chechi, K., Cooke, G.S., da Silva Filipe, A., de Silva, T., Docherty, A.B., dos Santos Correia, G., Dumas, M.-E., Dunning, J., Fletcher, T., Green, C.A., Greenhalf, W., Griffin, J.L., Gupta, R.K., Harrison, E.M., Hiscox, J.A., Ho, A.Y.W., Horby, P.W., Ijaz, S., Khoo, S., Klenerman, P., Law, A., Lewis, M.R., Liggi, S., Lim, W.S., Maslen, L., Mentzer, A.J., Merson, L., Meynert, A.M., Moore, S.C., Noursadeghi, M., Olanipekun, M., Osagie, A., Palmarini, M., Palmieri, C., Paxton, W.A., Pollakis, G., Price, N., Rambaut, A., Robertson, D.L., Russell, C.D., Sancho-Shimizu, V., Sands, C.J., Scott, J.T., Sigfrid, L., Solomon, T., Sriskandan, S., Stuart, D., Summers, C., Swann, O.V., Takats, Z., Takis, P., Tedder, R.S., Thompson, A.A.R., Thomson, E.C., Thwaites, R.S., Turtle, L.C.W., Zambon, M., Hardwick, H., Donohue, C., Griffiths, F., Oosthuyzen, W., Donegan, C., Spencer, R.G., Norman, L., Pius, R., Drake, T.M., Fairfield, C.J., Knight, S.R., Mclean, K.A., Murphy, D., Shaw, C.A., Dalton, J., Girvan, M., Saviciute, E., Roberts, S., Harrison, J., Marsh, L., Connor, M., Halpin, S., Jackson, C., Gamble, C., Plotkin, D., Lee, J., Leeming, G., Law, A., Wham, M., Clohisey, S., Hendry, R., Scott-Brown, J., Shaw, V., McDonald, S.E., Keating, S., Ahmed, K.A., Armstrong, J.A., Ashworth, M., Asiimwe, I.G., Bakshi, S., Barlow, S.L., Booth, L., Brennan, B., Bullock, K., Catterall, B.W.A., Clark, J.J., Clarke, E.A., Cole, S., Cooper, L., Cox, H., Davis, C., Dincarslan, O., Dunn, C., Dyer, P., Elliott, A., Evans, A., Finch, L., Fisher, L.W.S., Foster, T., Garcia-Dorival, I., Gunning, P., Jensen, R.L., Jones, C.B., Jones, T.R., Khandaker, S., King, K., Kiy, R.T., Koukorava, C., Lake, A., Lant, S., Latawiec, D., Lavelle-Langham, L., Lefteri, D., Lett, L., Livoti, L.A., Mancini, M., McDonald, S., McEvoy, L., McLauchlan, J., Metelmann, S., Miah, N.S., Middleton, J., Mitchell, J., Moore, S.C., Murphy, E.G., Pilgrim, J., Reynolds, W., Ridley, P.M., Sales, D., Shaw, V.E., Shears, R.K., Small, B., Subramaniam, K.S., Szemiel, A., Taggart, A., Tanianis-Hughes, J., Thomas, J., Trochu, E., van Tonder, L., Wilcock, E., Zhang, J.E., Flaherty, L., Maziere, N., Cass, E., Carracedo, A.D., Carlucci, N., Holmes, A., Massey, H., Murphy, L., McCafferty, S., Clark, R., Fawkes, A., Donnelly, L., MacGillivray, L., Bernatoniene, J., Berry, C., Chukkambotla, S., Collini, P., Cosgrove, C., Dark, P., Dushianthan, A., Finn, A., Gkrania-Klotsas, E., Hartshorn, S., Hawcutt, D.B., Hodgson, L., Hormis, A., Leiner, T., Lillie, P., Lyttle, M., Meda, M., Moon, J., Morgan, P., Moses, S., O’Shea, M.K., Ostermann, M., Patel, B., Pattison, N., Peters, M., Planche, T., Price, D., Shah, A., Sharma, A., Shankar-Hari, M., Smith, R., Szakmany, T., Tridente, A., Vancheeswaran, R., Welters, I., Whitehouse, T., Papineni, P., Wootton, D.G., Matthews, D.A., Darby, A.C., Hiscox, J.A.   +242 more
openaire   +10 more sources

Heat Treatment Promotes Ubiquitin-Mediated Proteolysis of SARS-CoV-2 RNA Polymerase and Decreases Viral Load

Research, 2022
Despite extensive efforts, COVID-19 pandemic caused by the SARS-CoV-2 virus is still at large. Vaccination is an effective approach to curb virus spread, but several variants (e.g., delta, delta plus, omicron, and IHU) appear to weaken or possibly escape
Yasen Maimaitiyiming, Tao Yang, Qian Qian Wang, Yan Feng, Zhi Chen, Mikael Björklund, Fudi Wang, Chonggao Hu, Chih-Hung Hsu, Hua Naranmandura   +9 more
doaj   +1 more source

High-throughput rational design of the remdesivir binding site in the RdRp of SARS-CoV-2: implications for potential resistance

iScience, 2021
Summary: The use of remdesivir to treat COVID-19 will likely continue before clinical trials are completed. Due to the lengthening pandemic and evolving nature of the virus, predicting potential residues prone to mutation is crucial for the management of
Aditya K. Padhi, Rohit Shukla, Prakash Saudagar, Timir Tripathi   +3 more
doaj   +1 more source