Results 71 to 80 of about 38,142 (142)

SARS-CoV-2 Point Mutation and Deletion Spectra and Their Association with Different Disease Outcomes

Microbiology Spectrum, 2022
Mutant spectra of RNA viruses are important to understand viral pathogenesis and response to selective pressures. There is a need to characterize the complexity of mutant spectra in coronaviruses sampled from infected patients.
Brenda Martínez-González, María Eugenia Soria, Lucía Vázquez-Sirvent, Cristina Ferrer-Orta, Rebeca Lobo-Vega, Pablo Mínguez, Lorena de la Fuente, Carlos Llorens, Beatriz Soriano, Ricardo Ramos, Marta Cortón, Rosario López-Rodríguez, Carlos García-Crespo, Isabel Gallego, Ana Isabel de Ávila, Jordi Gómez, Luis Enjuanes, Llanos Salar-Vidal, Jaime Esteban, Ricardo Fernandez-Roblas, Ignacio Gadea, Carmen Ayuso, Javier Ruíz-Hornillos, Nuria Verdaguer, Esteban Domingo, Celia Perales   +25 more
doaj   +1 more source

Implementation of a Multiplex PCR Amplification System Combined With Next‐Generation Genome Sequencing to Decipher the Circulation of Human Coronavirus 229E Lineages in Southern France

Journal of Medical Virology, Volume 97, Issue 11, November 2025.
ABSTRACT Coronaviruses rapidly evolve and are prone to new virus emergence. Human coronavirus (HCoV)−229E is one of the seven coronaviruses (aside HCoV‐OC43, HCoV‐HKU1, HCoV‐NL63, SARS‐CoV, MERS‐CoV, SARS‐CoV‐2) causing respiratory infections in humans. Genomic data are very scarce for this virus.
Houmadi Hikmat, Justine Py, Céline Boschi, Emilie Burel, Lorlane Le Targa, Matthieu Million, Lucile Lesage, Aurélie Morand, Bernard La Scola, Philippe Colson   +9 more
wiley   +1 more source

Increased flexibility of the SARS-CoV-2 RNA-binding site causes resistance to remdesivir.

PLoS Pathogens, 2023
Mutations continue to accumulate within the SARS-CoV-2 genome, and the ongoing epidemic has shown no signs of ending. It is critical to predict problematic mutations that may arise in clinical environments and assess their properties in advance to ...
Shiho Torii, Kwang Su Kim, Jun Koseki, Rigel Suzuki, Shoya Iwanami, Yasuhisa Fujita, Yong Dam Jeong, Jumpei Ito, Hiroyuki Asakura, Mami Nagashima, Kenji Sadamasu, Kazuhisa Yoshimura, Genotype to Phenotype Japan (G2P-Japan) Consortium, Kei Sato, Yoshiharu Matsuura, Teppei Shimamura, Shingo Iwami, Takasuke Fukuhara   +17 more
doaj   +1 more source

Drug repurposing screen to identify inhibitors of the RNA polymerase (nsp12) and helicase (nsp13) from SARS-CoV-2 replication and transcription complex

Virus Research
Coronaviruses contain one of the largest genomes among the RNA viruses, coding for 14–16 non-structural proteins (nsp) that are involved in proteolytic processing, genome replication and transcription, and four structural proteins that build the core of ...
Maria Kuzikov, Jeanette Reinshagen, Krzysztof Wycisk, Angela Corona, Francesca Esposito, Paolo Malune, Candida Manelfi, Daniela Iaconis, Andrea Beccari, Enzo Tramontano, Marcin Nowotny, Björn Windshügel, Philip Gribbon, Andrea Zaliani   +13 more
doaj   +1 more source

SARS-CoV-2 NSP12 utilizes various host splicing factors for replication and splicing regulation

, 2023
Abstract Background The RNA-dependent RNA polymerase (RdRp) is a crucial element in the replication and transcription of RNA viruses. Although the RdRps of lethal human coronaviruses SARS-CoV-2, SARS-CoV, and MARS-CoV have been extensively studied, the molecular mechanism of the catalytic subunit NSP12, which is involved in pathogenesis ...
Li Yang, Xiao-Tao Zeng, Si-Xue Ren, Lin-Lin Liang, Qiu-Xia Huang, Hong Fan, Hai-Yan Ren, Wei Cheng   +7 more
openaire   +1 more source

Crystallographic fragment screening against SARS‐CoV‐2 nonstructural protein 1 using the F2X‐Entry Screen and a newly developed fragment library

Acta Crystallographica Section D, Volume 81, Issue 11, Page 630-645, November 2025.
Two crystallographic fragment screening campaigns against SARS‐CoV‐2 nonstructural protein 1 resulted in the identification of 21 new hits.Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) continues to threaten global health. This underpins the need for novel therapeutics against this virus.
Frank Lennartz, Jan Wollenhaupt, Melanie Oelker, Paula Fröling, Uwe Mueller, Anke Deckers, Christoph Grathwol, Stefan Bräse, Nicole Jung, Manfred S. Weiss   +9 more
wiley   +1 more source

Rapid incorporation of Favipiravir by the fast and permissive viral RNA polymerase complex results in SARS-CoV-2 lethal mutagenesis

Nature Communications, 2020
Favipiravir (T-705) is an inhibitor of viral RNA-dependent-RNA-polymerases (RdRp) and clinical trials for the treatment of COVID-19 are ongoing. Here, the authors show that SARS-CoV nsp12 is the fastest known viral RdRp and they provide insights into the
Ashleigh Shannon, Barbara Selisko, Nhung-Thi-Tuyet Le, Johanna Huchting, Franck Touret, Géraldine Piorkowski, Véronique Fattorini, François Ferron, Etienne Decroly, Chris Meier, Bruno Coutard, Olve Peersen, Bruno Canard   +12 more
doaj   +1 more source

A New Fragment‐Based Pharmacophore Virtual Screening Workflow Identifies Potent Inhibitors of SARS‐CoV‐2 NSP13 Helicase

Journal of Computational Chemistry, Volume 46, Issue 23, September 5, 2025.
The new fragment‐based pharmacophore virtual screening workflow FragmentScout is introduced, which aggregates feature information present in each experimental fragment pose from XChem high‐throughput crystallographic screening. The method is applied to the SARS‐CoV‐2 NSP13 helicase, and we report the in silico discovery of 13 novel micromolar potent ...
Jordi Doijen, Jiexiong Xie, Simone Marsili, Trpta Bains, Mandeep Kaur Mann, Pravien Abeywickrema, Nick Van den Broeck, Christian Permann, Thierry Langer, Gökhan Ibis, Charles‐Alexandre Mattelaer, Jeremy Harvey, Sebastiaan van Raalte, Roberto Fino, Vineet Pande, Danielle Peeters, Aaron Patrick, Ellen Van Damme, Herman van Vlijmen, Marnix Van Loock, Edgar Jacoby   +20 more
wiley   +1 more source

NSP7 Molecular Degrader Attenuates Coronaviral Infection Through the β‐TrCP1/FBXO5 Axis

Advanced Science, Volume 12, Issue 36, September 25, 2025.
Host E3 ligase FBXO5 as a key regulator that promotes K48‐linked ubiquitination and proteasomal degradation of SARS‐CoV‐2 NSP7, thereby suppressing viral replication. NSP7 ubiquitination is co‐regulated by β‐TrCP1 and TAF1. Small molecule BC24877 disrupts β‐TrCP1‐mediated degradation of FBXO5, stabilizing FBXO5 and enhancing NSP7 clearance ...
Yao Tong, Travis B. Lear, Ferhan Tuncer, John J. Villandre, Áine N. Boudreau, Bo Lin, Irene Alfaras, Jason R. Kennerdell, Daniel P. Camarco, Mads B. Larsen, Yun Hua, Yanwen Chen, Meigin E. Chandler, Ricardo Pineda, Simon M. Barratt‐Boyes, John W. Evankovich, Toren Finkel, Yuan Liu, Bill B. Chen   +18 more
wiley   +1 more source

Spatial Transcriptomics and Single Cell‐RNASeq Reveals Cellular Heterogeneity of SARS‐CoV‐2 in Lung Tissues and Global Mutational Patterns in COVID‐19 Patients

Journal of Medical Virology, Volume 97, Issue 9, September 2025.
ABSTRACT RNA viruses have high mutation frequency, quick generation periods and vast population numbers, which promote fast evolution and host environment adaptation. We integrated scRNA‐seq and spatial transcriptomics to profile immune cells and viral gene expression in COVID‐19.
Seyed Taleb Hosseini, Mohammadamin Mahmanzar, Karim Rahimian, Saleha Bayat, Amir Gholamzad, Mahsa Mollapour Sisakht, Amin Farhadi, Donna Lee Kuehu, Youping Deng   +8 more
wiley   +1 more source