Results 61 to 70 of about 38,894 (153)

Genetic and Respiratory Pathogenic Comparison of Two PRRSV‐2 Isolates Classified as Lineage Korean A and B

Transboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
Porcine reproductive and respiratory syndrome virus (PRRSV) continues to cause substantial economic losses worldwide, and certain lineages circulate in specific countries. Lineage Korean A (LKA), B (LKB), and C (LKC) have been reported exclusively in Korea. However, studies on LKA and LKB have been limited to strains isolated before 2015. In this study,
Hyejin Na, Chanhee Chae, Nan-hua Chen
wiley   +1 more source

Five‐Year (2017–2022) Evolutionary Dynamics of Human Coronavirus OC43 in Southern France Based on Whole Genome Next‐Generation Sequencing

Journal of Medical Virology, Volume 97, Issue 12, December 2025.
ABSTRACT HCoV‐OC43 genomes and their evolution are scarcely studied worldwide and in France, with only 361 genomes available as of October 2023. Here, an in‐house PCR amplification system was implemented to obtain retrospectively by next‐generation sequencing, then analyze HCoV‐OC43 genomes for infections diagnosed with this virus in southern France ...
Hikmat Houmadi, Céline Boschi, Marine Lefebvre, Lorlane Le Targa, Justine Py, Jean‐Christophe Lagier, Lucile Lesage, Jacques Fantini, Aurélie Morand, Bernard La Scola, Philippe Colson   +10 more
wiley   +1 more source

Comparison of the effect of nsp12 on nsp13 activity using RNA and DNA substrates.

, 2012
(A, B): Time course of (A) RNA (31/18-mer) and (B) DNA (31/18-mer) unwinding by GST-nsp13 alone (▪), or by GST-nsp13 with nsp12 (♦) or GST-nsp13 with Foot-and-Mouth Disease Virus RNA polymerase (▴).
Eric J. Snijder (45119), Aartjan J. W. te Velthuis (164518), Susan Weiss (164526), Kamalendra Singh (164534), Robert L. Eoff (129297), Adeyemi O. Adedeji (164507), Bruno Marchand (164513), Stefan G. Sarafianos (164540)   +7 more
core   +1 more source

Engagement of nsp9_cov19 with the nsp12 NiRAN domain and VHH_2nsp23.

, 2023
A) Cartoon representation nsp9COV19 when engaged with the nsp12 NiRAN domain (10). B) Aligned nsp9COV19 structure when VHH2nsp23-bound demonstrating distortion of the NiRAN-engaging structural elements.
Indu R. Chandrashekaran (448750), Yue Pan (267516), Luke Tennant (15203526), Jamie Rossjohn (127997), Dene R. Littler (12088019)   +4 more
core   +1 more source

“Fertile” Mutations in SARS‐CoV‐2 RNA More Frequently Occurred in Hairpin Loops That Determine Virus Evolution

APMIS, Volume 133, Issue 12, December 2025.
ABSTRACT RNA hairpins may constitute a foundation of genetic evolution both in viruses and other organisms. Stem‐loops theoretically comprise a stable part, the double‐stranded stem, and a single‐stranded loop allowing evolution. Here we tested for SARS‐CoV‐2 if “fertile” mutations were in loops while mutations in stems were poorly tolerated and rarely
Philippe Colson, Pierre Pontarotti, Jacques Fantini, Anthony Levasseur, Christian Devaux, Didier Raoult   +5 more
wiley   +1 more source

Andrographolide binds to spike glycoprotein and RNA-dependent RNA polymerase (NSP12) of SARS-CoV-2 by in silico approach: a probable molecule in the development of anti-coronaviral drug

, 2021
The SARS-CoV-2 belongs to Coronaviridae family infects host cells by the interaction of its spike glycoprotein and angiotensin-converting enzyme 2 (ACE 2) of host cells. Upon entry, the virus uses its RNA dependent RNA polymerase (NSP12) for transcribing
Lokanathan Srikanth, Potukuchi Venkata Gurunadha Krishna Sarma   +1 more
core   +1 more source

Role of remdesivir for the treatment of COVID‐19 in patients with hematologic malignancy—A narrative review and expert opinion

European Journal of Clinical Investigation, Volume 55, Issue 12, December 2025.
Created in BioRender. Ghezzi, S. (2025) https://BioRender.com/q1lticg. Abstract Background COVID‐19 remains a potentially severe condition for immunocompromised individuals, such as patients with hematologic malignancy. These patients are at increased risk of progressing to severe–critical or prolonged COVID‐19.
Malgorzata Mikulska, Matteo Bassetti, Alessandro Busca, Valeria Cento, Maddalena Giannella, Michele Bartoletti   +5 more
wiley   +1 more source

A genus-specific nsp12 region impacts polymerase assembly in Alpha- and Gammacoronaviruses

Abstract Coronavirus relevancy for human health has surged over the past 20 years as they have a propensity for spillover into humans from animal reservoirs resulting in pandemics such as COVID-19. The diversity within the Coronavirinae subfamily and high infection frequency in ...
Peter J. Hoferle, Thomas K. Anderson, Robert N. Kirchdoerfer   +2 more
openaire   +2 more sources

Galectin-3 inhibits replication of porcine reproductive and respiratory syndrome virus by interacting with viral Nsp12 in vitro

Virus Research, 2018
Porcine galectin-3 (GAL3) is a 29-kDa protein encoded by a single gene, LGALS3, located on chromosome 1. Here, using a yeast two-hybrid screen of a cDNA library from porcine alveolar macrophage cells (PAMs), we report for the first time that GAL3 interacts with nonstructural protein 12 (Nsp12) of the porcine reproductive and respiratory syndrome virus (
Li, Liwei, Zhou, Yanjun, Jiang, Yifeng, Gao, Fei, Shan, Tongling, Zhao, Kuan, Zhang, Yujiao, Li, Lin, Tong, Guangzhi   +8 more
openaire   +2 more sources

The NSP5, ORF6 and NSP13 of SARS‐CoV‐2 Cooperate to Modulate Inflammatory Cell Death Activation

Advanced Science, Volume 12, Issue 41, November 6, 2025.
ZBP1 is capable of initiating a large cell death complex to induce programmed cell death during SARS‐CoV‐2 infection. However, SARS‐CoV‐2 can inhibit the activation of ZBP1‐mediated cell death by targeting key components of this complex. This suppression of ZBP1‐mediated cell death may account for the increased mortality observed in patients co ...
Huan Wang, Mengdi Liang, Jing Zhang, Hua Tong, Fenfen Zhang, Ying Liu, Pui Wang, Mengmeng Chang, Fei Han, Siwen Liu, Yongping Lin, Wenjun Song, Rajendra Karki, Peihui Wang, Honglin Chen, Yang Liu, Min Zheng   +16 more
wiley   +1 more source