Results 51 to 60 of about 3,842 (144)

Cooperative translocation enhances the unwinding of duplex DNA by SARS coronavirus helicase nsP13 [PDF]

Nucleic Acids Research, 2010
SARS coronavirus encodes non-structural protein 13 (nsP13), a nucleic acid helicase/NTPase belonging to superfamily 1 helicase, which efficiently unwinds both partial-duplex RNA and DNA. In this study, unwinding of DNA substrates that had different duplex lengths and 5'-overhangs was examined under single-turnover reaction conditions in the presence of
Lee, Na-Ra, Kwon, Hyun-Mi, Park, Kkothanahreum, Oh, Sangtaek, Jeong, Yong-Joo, Kim, Dong-Eun   +5 more
openaire   +2 more sources

Possible therapeutic targets and promising drugs based on unsymmetrical hetaryl-substituted porphyrins to combat SARS-CoV-2

Journal of Pharmaceutical Analysis, 2021
Coronavirus disease 2019 is a serious disease that causes acute respiratory syndrome and negatively affects the central nervous system. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) crosses the blood-brain barrier due to the spike (S ...
Yury A. Gubarev, Natalya Sh. Lebedeva, Elena S. Yurina, Sergey A. Syrbu, Aleksey N. Kiselev, Mikhail A. Lebedev   +5 more
doaj   +1 more source

Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Helicase

mSphere, 2016
Middle East respiratory syndrome coronavirus (MERS-CoV) helicase is a superfamily 1 helicase containing seven conserved motifs. We have cloned, expressed, and purified a Strep-fused recombinant MERS-CoV nonstructural protein 13 (M-nsp13) helicase ...
Adeyemi O. Adedeji, Hilary Lazarus
doaj   +1 more source

Transforming RNA Degradation into Accurate RNA Detection: RNase I–Assisted Rolling Circle Amplification for Clinical Samples

Angewandte Chemie, Volume 138, Issue 9, 23 February 2026.
Here, we transform this otherwise destructive enzymatic activity into a powerful diagnostic advantage through an RNase I–assisted rolling circle amplification (RI‐RCA) strategy. By integrating controlled RNase I–mediated RNA digestion with circular DNA templates, this approach enables direct and highly sensitive detection of target RNA sequences. Using
Amal Mathai, Cheng‐Yu Lee, Zijie Zhang, Jimmy Gu, John D. Brennan, Yingfu Li   +5 more
wiley   +2 more sources

Crystal structure of Middle East respiratory syndrome coronavirus helicase. [PDF]

PLoS Pathogens, 2017
Middle East respiratory syndrome coronavirus (MERS-CoV) remains a threat to public health worldwide; however, effective vaccine or drug against CoVs remains unavailable. CoV helicase is one of the three evolutionary most conserved proteins in nidoviruses,
Wei Hao, Justyna Aleksandra Wojdyla, Rong Zhao, Ruiyun Han, Rajat Das, Ivan Zlatev, Muthiah Manoharan, Meitian Wang, Sheng Cui   +8 more
doaj   +1 more source

Inhibitory potentials of ivermectin, nafamostat, and camostat on spike protein and some nonstructural proteins of SARS-CoV-2: Virtual screening approach

Jurnal Teknologi Laboratorium, 2022
The search for potent oral drugs either through synthetic routes or by drug repurposing for combating the dreaded covid-19 virus is still ongoing. The coronavirus spike glycoprotein and several other non-structural proteins play crucial roles in the ...
Haruna Isiyaku Umar, Ijeoma Akunna Duru, Uchechi Emmanuela Enenebeaku, Lynda Chioma Ngozi Olehi, Christian Ebere Enyoh, Chidi Edbert Duru   +5 more
doaj   +1 more source

Effective Binding Affinity of Inhibitor N-(3-(Carbamoylamino) Phenyl) Acetamide against the SARS-CoV-2 NSP13 Helicase

Medical Laboratory Journal, 2022
Background and objectives: The outbreak of coronavirus disease 2019 (COVID-19) has become a global health emergency. The severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) NSP13 helicase plays an important role in SARS-CoV-2 replication and ...
Rajneesh Prajapat, Suman Jain
doaj  

Coronavirus RNA-dependent RNA polymerase interacts with the p50 regulatory subunit of host DNA polymerase delta and plays a synergistic role with RNA helicase in the induction of DNA damage response and cell cycle arrest in the S phase

Emerging Microbes and Infections, 2023
Disruption of the cell cycle is a common strategy shared by many viruses to create a conducible cellular microenvironment for their efficient replication. We have previously shown that infection of cells with gammacoronavirus infectious bronchitis virus (
Li Quan, Xinxin Sun, Linghui Xu, Rui Ai Chen, Ding Xiang Liu   +4 more
doaj   +1 more source

SARS-CoV-2 Nsp13 Fragments and the Open Chemistry Network

, 2022
This is Matthew Todd's presentation at the monthly [virtual] Antiviral Drug Discovery (AViDD) Open Science Forum on 16 November 2022. Their talk centers around the druggability of SARS-CoV-2 nsp13. SGC nsp13 Open Lab Notebook You can find a recording of their talk on the AViDD Open Science Forum Youtube page Mat Todd is a part of the Rapidly Emerging ...
openaire   +2 more sources

Unnatural Amino Acid and Emerging Chemistry Approaches to Map RNA–Protein Interactions

Angewandte Chemie, Volume 138, Issue 6, 2 February 2026.
This review highlights emerging chemistries for mapping RNA–protein interactions, including genetically encoded unnatural amino acids, novel photocrosslinkers, and non‐photoactivatable crosslinking systems. We compare their mechanisms, reactivity and applications, outlining how these next‐generation tools enable higher‐resolution, site‐specific ...
Eryn Lundrigan, Parrish Evers, Elexa Scott, John Paul Pezacki   +3 more
wiley   +2 more sources