Results 71 to 80 of about 7,665 (202)

SARS‐CoV‐2 targets mitochondria, exacerbating COVID‐19 pneumonia

The Journal of Physiology, EarlyView.
Abstract figure legend Following entry into airway epithelial cells (AECs), SARS‐CoV‐2 releases its single‐stranded RNA into the cytoplasm, where it is translated into viral proteins. Several of these viral proteins localize to mitochondria and interact with key mitochondrial components.
Danchen Wu, Asish Dasgupta, Jaskaran Singh Hora, Kuang‐Hueih Chen, Arinjay Banerjee, Stephen L. Archer   +5 more
wiley   +1 more source

Human enteroids: Preclinical models of non-inflammatory diarrhea [PDF]

, 2013
Researchers need an available and easy-to-use model of the human intestine to better understand human intestinal physiology and pathophysiology of diseases, and to offer an enhanced platform for developing drug therapy.
Broughman, J.R. (James), Donowitz, M. (Mark), Estes, M. (Mary), Ettayebi, K. (Khalil), Foulke-Abel, J. (Jennifer), Hyser, J.M. (Joseph), In, J. (Julie), Jonge, H.R. (Hugo) de, Kovbasnjuk, O. (Olga), Middendorp, S., Zachos, N.C. (Nicholas C.), Zeng, X.-L. (Xi-Lei)   +11 more
core   +1 more source

A VSV-based assay quantifies coronavirus Mpro/3CLpro/Nsp5 main protease activity and chemical inhibition

Communications Biology, 2022
A vesicular stomatitis virus (VSV)-based assay enables high-throughput screening for small molecular protease inhibitors that can block viral proteases, like the Mpro/3CLpro/Nsp5 in SARS-CoV-2.
Emmanuel Heilmann, Francesco Costacurta, Stephan Geley, Seyad Arad Mogadashi, Andre Volland, Bernhard Rupp, Reuben Stewart Harris, Dorothee von Laer   +7 more
doaj   +1 more source

The Open Reading Frame 7b of the SARS‐CoV‐2 Disperse Trans‐Golgi and Activate the NLRP3 Inflammasome

Journal of Medical Virology, Volume 98, Issue 4, April 2026.
ABSTRACT Inflammasomes orchestrate the inflammatory response against bacterial and viral infections, thereby initiating the synthesis of pro‐inflammatory cytokines, mainly IL‐1β and IL‐18. SARS‐CoV‐2 infection induces an inflammatory response mediated by the activation of NLRP1 and NLRP3 inflammasomes.
Julio García‐Villalba, Laura Hurtado‐Navarro, Diego Angosto‐Bazarra, Alberto Baroja‐Mazo, Pablo Pelegrin   +4 more
wiley   +1 more source

Assessing Genomic Mutations in SARS-CoV-2: Potential Resistance to Antiviral Drugs in Viral Populations from Untreated COVID-19 Patients

Microorganisms, 2023
Naturally occurring SARS-CoV-2 variants mutated in genomic regions targeted by antiviral drugs have not been extensively studied. This study investigated the potential of the RNA-dependent RNA polymerase (RdRp) complex subunits and non-structural protein
Daniele Lombardo, Cristina Musolino, Valeria Chines, Giuseppe Caminiti, Claudia Palermo, Irene Cacciola, Giuseppina Raffa, Teresa Pollicino   +7 more
doaj   +1 more source

Liquid–Liquid Phase Separation in Viral Infection and Immunology

MedComm, Volume 7, Issue 4, April 2026.
LLPS organizes viral replication and antiviral immunity. Viruses hijack LLPS to form replication factories and evade immune sensors, while hosts assemble LLPS‐driven signaling hubs (e.g., MAVS, RIG‐I, and SGs) to amplify interferon responses. Targeting these condensate interfaces offers novel therapeutic strategies against infectious diseases ...
Jiuzhi Xu, Lan Bai, Bin Wang, Hai Song, Long Zhang, Fangfang Zhou   +5 more
wiley   +1 more source

Antivirals Targeting Coronavirus RNA‐Dependent RNA Polymerase and Main Protease: From Mechanisms of Action to Outcomes in COVID‐19 Clinical Trials

Microbial Biotechnology, Volume 19, Issue 4, April 2026.
The target sites of COVID‐19 antivirals discussed in the present opinion paper, namely the RNA dependent RNA polymerase Nsp12 and of the main viral protease Nsp5, are indicated by a red star in the overview of the replication cycle of coronavirus SARS‐CoV‐2.
Harald Brüssow
wiley   +1 more source

NSP5 phosphorylation regulates the fate of viral mRNA in rotavirus infected cells

Archives of Virology, 2002
Elucidation of the function of the non-structural rotavirus proteins during infection is difficult in the absence of a reverse genetic system. To study the role of NSP5, nonstructural phosphoprotein NSP5, we constructed a reassortant strain (SACC11) in the SA11 background that harbours a heterologous segment 11 encoding a variant protein (h-NSP5 ...
Chnaiderman, J, Barro, M, Spencer, E
openaire   +4 more sources

Sequence Analysis and Structural Prediction of the Severe Acute Respiratory Syndrome Coronavirus nsp5 [PDF]

Acta Biochimica et Biophysica Sinica, 2005
The non-structural proteins (nsp or replicase proteins) of coronaviruses are relatively conserved and can be effective targets for drugs. Few studies have been conducted into the function of the severe acute respiratory syndrome coronavirus (SARS-CoV) nsp5.
Jia-Hai, Lu, Ding-Mei, Zhang, Guo-Ling, Wang, Zhong-Min, Guo, Juan, Li, Bing-Yan, Tan, Li-Ping, Ou-Yang, Wen-Hua, Ling, Xin-Bing, Yu, Nan-Shan, Zhong   +9 more
openaire   +2 more sources

Whole‐Genome Characterization of Rare Artiodactyl‐Like G10P [14] and G8P [14] Rotavirus A Strains Detected in Pediatric Gastroenteritis Cases in Hokkaido Prefecture, Japan

Journal of Medical Virology, Volume 98, Issue 3, March 2026.
ABSTRACT Rotavirus alphagastroenteritidis (RVA) is the leading cause of acute gastroenteritis in young children worldwide, as well as in a wide range of animal species. In 2020 and 2022, we identified three unusual RVA strains in pediatric gastroenteritis patients in Hokkaido Prefecture, Japan, using next‐generation sequencing: one G10P[14] strain ...
Akira Takebayashi, Yuya Fukuda, Yoshiki Fujii, Hiroshi Nihira, Minako Kihara, Yoshimasa Kudou, Shuhei Adachi, Noritaka Shintani, Takeshi Tsugawa   +8 more
wiley   +1 more source