Results 61 to 70 of about 75,707 (295)
Nuclear Import of Adenovirus DNA in Vitro Involves the Nuclear Protein Import Pathway and hsc70 [PDF]
Journal of Biological Chemistry, 2000 Adenovirus, a respiratory virus with a double-stranded DNA genome, replicates in the nuclei of mammalian cells. We have developed a cytosol-dependent in vitro assay utilizing adenovirus nucleocapsids to examine the requirements for adenovirus docking to the nuclear pore complex and for DNA import into the nucleus.
A C, Saphire +4 more
openaire +2 more sources
FEBS Open Bio, EarlyView.Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero +5 more
wiley +1 more source
HIV-1 exploits importin 7 to maximize nuclear import of its DNA genome
Retrovirology, 2009 Background Nuclear import of the HIV-1 reverse transcription complex (RTC) is critical for infection of non dividing cells, and importin 7 (imp7) has been implicated in this process.
Leyens Lada +5 more
doaj +1 more source
The Journal of Cell Biology, 2006 ![][1] In vitro nuclear transport (top) works only with lysate and ATP. GERACE The nuclear pore serves as the gateway to the nucleus, allowing molecules to slip in and out. A technique devised by Larry Gerace (Scripps Research Institute in La Jolla, California) and colleagues ...
openaire +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Analysis of the Activity of Nuclear Export Signals Using Fluorescent BSA Conjugates
BioTechniques, 2002 Here we demonstrate that fluorescein-labeled BSA conjugated with a mixture of nuclear import and export signals can be used to evaluate export activity.
Carl M. Feldherr, Debra Akin
doaj +1 more source
Nuclear Import of Viral DNA Genomes [PDF]
Traffic, 2003 The genomes of many viruses traffic into the nucleus, where they are either integrated into host chromosomes or maintained as episomal DNA and then transcriptionally activated or silenced. Here, we discuss the existing evidence on how the lentiviruses, adenoviruses, herpesviruses, hepadnaviruses and autonomous parvoviruses enter the nucleus.
Greber, U F, Fassati, A
openaire +4 more sources
Cutaneous Melanoma Drives Metabolic Changes in the Aged Bone Marrow Immune Microenvironment
Aging and Cancer, EarlyView.Melanoma, the deadliest form of skin cancer, increasingly affects older adults. Our study reveals that melanoma induces changes in iron and lipid levels in the bone marrow, impacting immune cell populations and increasing susceptibility to ferroptosis.
Alexis E. Carey +12 more
wiley +1 more source
The Structure and Function of GLFGs in the Nuclear Pore Complex of Yeast [PDF]
, 2012 The nuclear pore complex (NPC) is a large multiprotein complex which perforates the nuclear envelope. The NPC is made up of nuclear pore proteins (Nups), one third of which are phenylalanine-glycine (FG) containing.
SPINK, MATTHEW,CHARLES
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