Results 11 to 20 of about 345,890 (219)

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

Frontiers in Molecular Biosciences, 2021
The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects.
Nadide Altincekic, Nadide Altincekic, Sophie Marianne Korn, Sophie Marianne Korn, Nusrat Shahin Qureshi, Nusrat Shahin Qureshi, Marie Dujardin, Martí Ninot-Pedrosa, Rupert Abele, Marie Jose Abi Saad, Caterina Alfano, Fabio C. L. Almeida, Fabio C. L. Almeida, Islam Alshamleh, Islam Alshamleh, Gisele Cardoso de Amorim, Gisele Cardoso de Amorim, Thomas K. Anderson, Cristiane D. Anobom, Cristiane D. Anobom, Chelsea Anorma, Jasleen Kaur Bains, Jasleen Kaur Bains, Adriaan Bax, Martin Blackledge, Julius Blechar, Julius Blechar, Anja Böckmann, Louis Brigandat, Anna Bula, Matthias Bütikofer, Aldo R. Camacho-Zarco, Teresa Carlomagno, Teresa Carlomagno, Icaro Putinhon Caruso, Icaro Putinhon Caruso, Icaro Putinhon Caruso, Betül Ceylan, Betül Ceylan, Apirat Chaikuad, Apirat Chaikuad, Feixia Chu, Laura Cole, Marquise G. Crosby, Vanessa de Jesus, Vanessa de Jesus, Karthikeyan Dhamotharan, Karthikeyan Dhamotharan, Isabella C. Felli, Isabella C. Felli, Jan Ferner, Jan Ferner, Yanick Fleischmann, Marie-Laure Fogeron, Nikolaos K. Fourkiotis, Christin Fuks, Boris Fürtig, Boris Fürtig, Angelo Gallo, Santosh L. Gande, Santosh L. Gande, Juan Atilio Gerez, Dhiman Ghosh, Francisco Gomes-Neto, Francisco Gomes-Neto, Oksana Gorbatyuk, Serafima Guseva, Carolin Hacker, Sabine Häfner, Bing Hao, Bruno Hargittay, Bruno Hargittay, K. Henzler-Wildman, Jeffrey C. Hoch, Katharina F. Hohmann, Katharina F. Hohmann, Marie T. Hutchison, Marie T. Hutchison, Kristaps Jaudzems, Katarina Jović, Janina Kaderli, Gints Kalniņš, Iveta Kaņepe, Robert N. Kirchdoerfer, John Kirkpatrick, John Kirkpatrick, Stefan Knapp, Stefan Knapp, Robin Krishnathas, Robin Krishnathas, Felicitas Kutz, Felicitas Kutz, Susanne zur Lage, Roderick Lambertz, Andras Lang, Douglas Laurents, Lauriane Lecoq, Verena Linhard, Verena Linhard, Frank Löhr, Frank Löhr, Anas Malki, Luiza Mamigonian Bessa, Rachel W. Martin, Rachel W. Martin, Tobias Matzel, Tobias Matzel, Damien Maurin, Seth W. McNutt, Nathane Cunha Mebus-Antunes, Nathane Cunha Mebus-Antunes, Beat H. Meier, Nathalie Meiser, Miguel Mompeán, Elisa Monaca, Roland Montserret, Laura Mariño Perez, Celine Moser, Claudia Muhle-Goll, Thais Cristtina Neves-Martins, Thais Cristtina Neves-Martins, Xiamonin Ni, Xiamonin Ni, Brenna Norton-Baker, Roberta Pierattelli, Roberta Pierattelli, Letizia Pontoriero, Letizia Pontoriero, Yulia Pustovalova, Oliver Ohlenschläger, Julien Orts, Andrea T. Da Poian, Dennis J. Pyper, Dennis J. Pyper, Christian Richter, Christian Richter, Roland Riek, Chad M. Rienstra, Angus Robertson, Anderson S. Pinheiro, Anderson S. Pinheiro, Raffaele Sabbatella, Nicola Salvi, Krishna Saxena, Krishna Saxena, Linda Schulte, Linda Schulte, Marco Schiavina, Marco Schiavina, Harald Schwalbe, Harald Schwalbe, Mara Silber, Marcius da Silva Almeida, Marcius da Silva Almeida, Marc A. Sprague-Piercy, Georgios A. Spyroulias, Sridhar Sreeramulu, Sridhar Sreeramulu, Jan-Niklas Tants, Jan-Niklas Tants, Kaspars Tārs, Felix Torres, Sabrina Töws, Miguel Á. Treviño, Sven Trucks, Aikaterini C. Tsika, Krisztina Varga, Ying Wang, Marco E. Weber, Julia E. Weigand, Christoph Wiedemann, Julia Wirmer-Bartoschek, Julia Wirmer-Bartoschek, Maria Alexandra Wirtz Martin, Maria Alexandra Wirtz Martin, Johannes Zehnder, Martin Hengesbach, Andreas Schlundt, Andreas Schlundt   +178 more
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ssNMRlib: a comprehensive library and tool box for acquisition of solid-state nuclear magnetic resonance experiments on Bruker spectrometers [PDF]

Magnetic Resonance, 2020
We introduce ssNMRlib, a comprehensive suite of pulse sequences and jython scripts for user-friendly solid-state nuclear magnetic resonance (NMR) data acquisition, parameter optimization and storage on Bruker spectrometers.
A. Vallet, A. Favier, B. Brutscher, P. Schanda   +3 more
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Discovery of 5,5′-Methylenedi-2,3-Cresotic Acid as a Potent Inhibitor of the Chemotactic Activity of the HMGB1·CXCL12 Heterocomplex Using Virtual Screening and NMR Validation

Frontiers in Chemistry, 2020
HMGB1 is a key molecule that both triggers and sustains inflammation following infection or injury, and is involved in a large number of pathologies, including cancer.
Federica De Leo, Giacomo Quilici, Francesco De Marchis, Malisa Vittoria Mantonico, Marco Emilio Bianchi, Marco Emilio Bianchi, Giovanna Musco   +6 more
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Structural determination of biomolecular interfaces by nuclear magnetic resonance of proteins with reduced proton density [PDF]

Journal of Biomolecular NMR, 2010
Protein interactions are important for understanding many molecular mechanisms underlying cellular processes. So far, interfaces between interacting proteins have been characterized by NMR spectroscopy mostly by using chemical shift perturbations and cross-saturation via intermolecular cross-relaxation.
Ferrage, Fabien, Dutta, Kaushik, Shekhtman, Alexander, Cowburn, David   +3 more
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Site-Specific Introduction of Alanines for the Nuclear Magnetic Resonance Investigation of Low-Complexity Regions and Large Biomolecular Assemblies

ACS Chemical Biology, 2023
Nuclear magnetic resonance (NMR) studies of large biomolecular machines and highly repetitive proteins remain challenging due to the difficulty of assigning frequencies to individual nuclei. Here, we present an efficient strategy to address this challenge by engineering a Pyrococcus horikoshii tRNA/alanyl-tRNA synthetase pair that enables the ...
Carlos A. Elena-Real, Annika Urbanek, Lionel Imbert, Anna Morató, Aurélie Fournet, Frédéric Allemand, Nathalie Sibille, Jérôme Boisbouvier, Pau Bernadó   +8 more
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ChemInform Abstract: Recent Advances in Solid‐State Nuclear Magnetic Resonance Techniques to Quantify Biomolecular Dynamics [PDF]

ChemInform, 2013
AbstractReview: 52 refs.
Chad M. Rienstra, Eric D. Watt
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Driving force of biomolecular liquid–liquid phase separation probed by nuclear magnetic resonance spectroscopy

Biophysics Reports, 2021
The assembly of biomolecular condensates is driven by liquid-liquid phase separation. To understand the structure and functions of these condensates, it is essential to characterize the underlying driving forces, e.g., protein-protein and protein-RNA interactions.
Zhang, Hanyu, Fan, Weiwei, Nshogoza, Gilbert, Liu, Yaqian, Gao, Jia, Wu, Jihui, Shi, Yunyu, Tu, Xiaoming, Zhang, Jiahai, Ruan, Ke   +9 more
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Synthesis of lanthanide tag and experimental studies on paramagnetically induced residual dipolar couplings

BMC Chemistry, 2022
Nuclear Magnetic Resonance (NMR) spectroscopy is an indispensable technique for the structure elucidation of molecules and determination of their characteristic interactions.
Ali Yassin, Bilal Nehmeh, Sally El Kantar, Yara Al Kazzaz, Elias Akoury   +4 more
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Integrating a prospective pilot trial and patient-derived xenografts to trace metabolic changes associated with acute myeloid leukemia

Journal of Hematology & Oncology, 2016
Despite the considerable progress in understanding the molecular bases of acute myeloid leukemia (AML), new tools to link disease biology to the unpredictable patient clinical course are still needed.
Matteo G. Carrabba, Laurette Tavel, Giacomo Oliveira, Alessandra Forcina, Giacomo Quilici, Francesca Nardelli, Cristina Tresoldi, Alessandro Ambrosi, Fabio Ciceri, Massimo Bernardi, Luca Vago, Giovanna Musco   +11 more
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¹⁷O NMR Spectroscopy: A Novel Probe for Characterizing Protein Structure and Folding

Biology, 2021
Oxygen is a key atom that maintains biomolecular structures, regulates various physiological processes, and mediates various biomolecular interactions. Oxygen-17 (17O), therefore, has been proposed as a useful probe that can provide detailed information ...
Srinivasan Muniyappan, Yuxi Lin, Young-Ho Lee, Jin Hae Kim   +3 more
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