Results 91 to 100 of about 2,343,531 (340)

Nuclear pore density controls heterochromatin reorganization during senescence

bioRxiv, 2018
Oncogene induced senescence (OIS) is a cell cycle arrest program triggered by oncogenic signalling. An important characteristic of OIS is activation of the senescence associated secretory phenotype (SASP)1 which can reinforce cell cycle arrest, lead to ...
Charlene Boumendilrid, P. Hari, K. Olsen, J. Acosta, W. Bickmore   +4 more
semanticscholar   +1 more source

Structure-function mapping of a heptameric module in the nuclear pore complex. [PDF]

, 2012
The nuclear pore complex (NPC) is a multiprotein assembly that serves as the sole mediator of nucleocytoplasmic exchange in eukaryotic cells. In this paper, we use an integrative approach to determine the structure of an essential component of the yeast ...
Chait, Brian T, Diaz-Avalos, Ruben, Fernandez-Martinez, Javier, Franke, Josef D, Phillips, Jeremy, Rout, Michael P, Sali, Andrej, Sekedat, Matthew D, Stokes, David L, Velazquez-Muriel, Javier, Williams, Rosemary   +10 more
core   +2 more sources

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source

CDK12 Activity-Dependent Phosphorylation Events in Human Cells

Biomolecules, 2019
We asked whether the C-terminal repeat domain (CTD) kinase, CDK12/CyclinK, phosphorylates substrates in addition to the CTD of RPB1, using our CDK12analog-sensitive HeLa cell line to investigate CDK12 activity-dependent phosphorylation events in human ...
Bartlomiej Bartkowiak, Christopher M. Yan, Erik J. Soderblom, Arno L. Greenleaf   +3 more
doaj   +1 more source

Simple rules for passive diffusion through the nuclear pore complex

Journal of Cell Biology, 2016
Passive macromolecular diffusion through nuclear pore complexes is thought to decrease dramatically beyond ∼40 kD. Using time-resolved fluorescence microscopy and Brownian dynamics simulations, Timney et al. show that this barrier is in fact much softer,
Benjamin L Timney, Barak Raveh, Roxana Mironska, Jill Trivedi, Seung Joong Kim, Daniel Russel, S. Wente, A. Sali, M. Rout   +8 more
semanticscholar   +1 more source

AFM imaging of functionalized carbon nanotubes on biological membranes [PDF]

, 2009
Multifunctional carbon nanotubes are promising for biomedical applications as their nano-size, together with their physical stability, gives access into the cell and various cellular compartments including the nucleus.
Coley, Helen M., Danzberger, Jürgen, Ebner, Andreas, Flahaut, Emmanuel, Gruber, Hermann J., Heister, Elena, Hinterdorfer, Peter, Kienberger, Ferry, Lamprecht, Constanze, Liashkovich, I., McFadden, Johnjoe, Neves, Vera, Rangl, Martina   +12 more
core   +1 more source

Refining the NaV1.7 pharmacophore of a class of venom‐derived peptide inhibitors via a combination of in silico screening and rational engineering

FEBS Letters, EarlyView.
Venom peptides have shown promise in treating pain. Our study uses computer screening to identify a peptide that targets a sodium channel (NaV1.7) linked to chronic pain. We produced the peptide in the laboratory and refined its design, advancing the search for innovative pain therapies.
Gagan Sharma, Jennifer R. Deuis, Xinying Jia, Theo Crawford, Sassan Rahnama, Eivind A. B. Undheim, Irina Vetter, Yanni K. ‐Y. Chin, Mehdi Mobli   +8 more
wiley   +1 more source

A thermodynamic paradigm for solution demixing inspired by nuclear transport in living cells [PDF]

Phys. Rev. Lett. 118, 158101 (2017), 2016
Living cells display a remarkable capacity to compartmentalize their functional biochemistry. A particularly fascinating example is the cell nucleus. Exchange of macromolecules between the nucleus and the surrounding cytoplasm does not involve traversing a lipid bilayer membrane.
arxiv   +1 more source

Rescue of DNA damage after constricted migration reveals a mechano-regulated threshold for cell cycle. [PDF]

, 2019
Migration through 3D constrictions can cause nuclear rupture and mislocalization of nuclear proteins, but damage to DNA remains uncertain, as does any effect on cell cycle.
Bannister, Beadle, Belin, Bennett, Britton, Buxboim, Charlotte R. Pfeifer, Cho, Cornelius, Cumming, Dazhen Liu, Denais, Dennis E. Discher, Dong, Duheron, Emily J. Chen, Frigault, Garay, Giese, Gomes, Grundy, Harada, Harding, Hatch, Houtgraaf, Imlay, Irena L. Ivanovska, Irianto, Irianto, Ivkovic, Jerome Irianto, Kalia Pannell, Khatau, Kryston, Kuangzheng Zhu, Lawrence J. Dooling, Lee, Liu, Liu, Lu, Lucas R. Smith, López-Bigas, Maciejowski, Mahaseth, Mai Wang, Mavrakis, Mello Filho, Michael P. Tobin, Mogilner, Morita, Pajerowski, Pathak, Petrie, Pfeifer, Pfeifer, Raab, Raab, Radisky, Rancati, Rice, Rico, Rizvi, Roger A. Greenberg, Sancar, Skamagki, Strano, Stroka, Surcel, Takaki, Thiam, Thomas, Turgay, Wang, Wolf, Xia, Yan, Yuntao Xia, Zhang   +77 more
core   +1 more source

The power of microRNA regulation—insights into immunity and metabolism

FEBS Letters, EarlyView.
MicroRNAs are emerging as crucial regulators at the intersection of metabolism and immunity. This review examines how miRNAs coordinate glucose and lipid metabolism while simultaneously modulating T‐cell development and immune responses. Moreover, it highlights how cutting‐edge artificial intelligence applications can identify miRNA biomarkers ...
Stefania Oliveto, Nicola Manfrini, Stefano Biffo   +2 more
wiley   +1 more source