Results 91 to 100 of about 326,922 (335)

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Meiotic cellular rejuvenation is coupled to nuclear remodeling in budding yeast. [PDF]

, 2019
Production of healthy gametes in meiosis relies on the quality control and proper distribution of both nuclear and cytoplasmic contents. Meiotic differentiation naturally eliminates age-induced cellular damage by an unknown mechanism.
Chetlapalli, Keerthana, Goodman, Jay S, Jorgens, Danielle M, King, Grant A, McDonald, Kent L, Schick, Jennifer G, Ünal, Elçin   +6 more
core   +1 more source

Three-dimensional structure and flexibility of a membrane-coating module of the nuclear pore complex. [PDF]

, 2009
The nuclear pore complex mediates nucleocytoplasmic transport in all eukaryotes and is among the largest cellular assemblies of proteins, collectively known as nucleoporins. Nucleoporins are organized into distinct subcomplexes.
Blobel, Günter, Kampmann, Martin
core   +2 more sources

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

MODELLING THE INFLUENCE OF NUCLEUS ELASTICITY ON CELL INVASION IN FIBER NETWORKS AND MICROCHANNELS [PDF]

, 2013
Cell migration in highly constrained extracellular matrices is exploited in scaffold-based tissue engineering and is fundamental in a wide variety of physiological and pathological phenomena, among others in cancer invasion and development. Research into
Preziosi, Luigi, Scianna, Marco
core   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore

eLife, 2018
Non-centrosomal microtubule organizing centers (MTOCs) are important for microtubule organization in many cell types. In fission yeast Schizosaccharomyces pombe, the protein Mto1, together with partner protein Mto2 (Mto1/2 complex), recruits the γ ...
Xun X Bao, Christos Spanos, Tomoko Kojidani, Eric M Lynch, Juri Rappsilber, Yasushi Hiraoka, Tokuko Haraguchi, Kenneth E Sawin   +7 more
doaj   +1 more source

Exportins can inhibit major mitotic assembly events in vitro: membrane fusion, nuclear pore formation, and spindle assembly

Nucleus, 2020
Xenopus egg extracts are a powerful in vitro tool for studying complex biological processes, including nuclear reconstitution, nuclear membrane and pore assembly, and spindle assembly.
Matthew S. Nord, Cyril Bernis, Sarah Carmona, Dennis C. Garland, Anna Travesa, Douglass J. Forbes   +5 more
doaj   +1 more source

Nucleocytoplasmic transport: a thermodynamic mechanism

, 2009
The nuclear pore supports molecular communication between cytoplasm and nucleus in eukaryotic cells. Selective transport of proteins is mediated by soluble receptors, whose regulation by the small GTPase Ran leads to cargo accumulation in, or depletion ...
Adam SA, Akey CW, Alber F, Bapteste E, Beck M, Becskei A, Ben‐Efraim I, Bickel T, Caspi Y, Catimel B, Cingolani G, Coppey M, Costa M, Cronshaw JM, Dange T, Denning DP, Devos D, Dixon M, Fanara P, Fradin C, Fradin C, Frey S, Grünwald D, Görlich D, Haldane JBS, Harreman MT, Henderson GP, Hinshaw JE, Hodel AE, Hu W, Hübner S, Jovanovic‐Talisman T, Kalab P, Kalderon D, Kapon R, Kobe B, Kopito RB, Kose S, Kotera I, Kubitscheck U, Kustanovich T, Köhler M, Lam MH, Lee SJ, Lyman SK, Mans BJ, Melcák I, Michael Elbaum, Michael WM, Miyamoto Y, Nachury MV, Naim B, Nakielny S, Newmeyer DD, Ossareh‐Nazari B, Pederson T, Pollard VW, Pyhtila B, Ribbeck K, Ribbeck K, Riddick G, Riddick G, Rihs HP, Robbins J, Ronen Benjamine Kopito, Rout MP, Salman H, Schmidt‐Zachmann MS, Schwartz TU, Shah S, Smith A, Stade K, Steggerda SM, Stein WD, Stoffler D, Strawn LA, Timney BL, Tokunaga M, Wen W, Yang Q, Yang W, Yang W, Zeitler B, Zilman A   +83 more
core   +2 more sources

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source