Results 71 to 80 of about 1,947,312 (301)
Organellar proteomics: the prizes and pitfalls of opening the nuclear envelope [PDF]
, 2002 Proteomic studies have the potential to comprehensively define the composition of organelles but are limited by the organellar cross-contamination that arises during subcellular fractionation.
Gerace, Larry, Schirmer, Eric C
core +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
CDK12 Activity-Dependent Phosphorylation Events in Human Cells
Biomolecules, 2019 We asked whether the C-terminal repeat domain (CTD) kinase, CDK12/CyclinK, phosphorylates substrates in addition to the CTD of RPB1, using our CDK12analog-sensitive HeLa cell line to investigate CDK12 activity-dependent phosphorylation events in human ...
Bartlomiej Bartkowiak +3 more
doaj +1 more source
Cells, 2021 Hutchinson-Gilford progeria syndrome (HGPS) is a rare premature aging disease caused by a mutation in LMNA. A G608G mutation in exon 11 of LMNA is responsible for most HGPS cases, generating a truncated protein called “progerin”.
Jennifer M. Röhrl +2 more
doaj +1 more source
The interplay of nuclear pores and lipids
Current Opinion in Cell Biology, 2023 Nuclear pore complexes (NPCs) mediate the bidirectional transport of cargo across the nuclear envelope (NE). NPCs are also membrane remodeling machines with a capacity to curve and fuse the membranes of the NE. However, little is known about the interplay of NPCs and lipids at a mechanistic level.
Edvinas Stankunas, Alwin Köhler
openaire +2 more sources
Electron tomography reveals posttranscriptional binding of pre-mRNPs to specific fibers in the nucleoplasm [PDF]
, 2000 International audienceUsing electron tomography, we have analyzed whether the Balbiani ring (BR) pre-mRNP particles in transit from the gene to the nuclear pore complex (NPC) are bound to any structure that could impair free diffusion through the ...
Sabri, N +21 more
core +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Molecular genetics of oogenesis in Drosophila melanogaster [PDF]
Invertebrate Survival Journal, 2004 Gonadal development requires complex differentiation programs leading to the production offunctional female and male gametes. Upon fertilization, while the male germ cell contributes to thenewly formed zygote only its genetic material, the female germ ...
S Gigliotti +4 more
doaj +2 more sources
Insights into Adenovirus Uncoating from Interactions with Integrins and Mediators of Host Immunity
Viruses, 2016 Human adenoviruses are large (150 MDa) nonenveloped double-stranded DNA (dsDNA) viruses that cause acute respiratory, gastrointestinal and ocular infections.
Glen R. Nemerow, Phoebe L. Stewart
doaj +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source