Results 61 to 70 of about 159,475 (307)

Detailed characterisation of the trypanosome nuclear pore architecture reveals conserved asymmetrical functional hubs that drive mRNA export.

PLoS Biology
Nuclear export of mRNAs requires loading the mRNP to the transporter Mex67/Mtr2 in the nucleoplasm, controlled access to the pore by the basket-localised TREX-2 complex and mRNA release at the cytoplasmic site by the DEAD-box RNA helicase Dbp5 ...
Bernardo Papini Gabiatti, Johanna Krenzer, Silke Braune, Timothy Krüger, Martin Zoltner, Susanne Kramer   +5 more
doaj   +1 more source

An Efficient Method for Isolating and Purifying Nuclei from Mice Brain for Single-Molecule Imaging Using High-Speed Atomic Force Microscopy

Cells
Nuclear pore complexes (NPCs) on the nuclear membrane surface have a crucial function in controlling the movement of small molecules and macromolecules between the cell nucleus and cytoplasm through their intricate core channel resembling a spiderweb ...
Yujia Qiu, Elma Sakinatus Sajidah, Sota Kondo, Shinnosuke Narimatsu, Muhammad Isman Sandira, Yoshiki Higashiguchi, Goro Nishide, Azuma Taoka, Masaharu Hazawa, Yuka Inaba, Hiroshi Inoue, Ayami Matsushima, Yuki Okada, Mitsutoshi Nakada, Toshio Ando, Keesiang Lim, Richard W. Wong   +16 more
doaj   +1 more source

The Structure and Function of GLFGs in the Nuclear Pore Complex of Yeast [PDF]

, 2012
The nuclear pore complex (NPC) is a large multiprotein complex which perforates the nuclear envelope. The NPC is made up of nuclear pore proteins (Nups), one third of which are phenylalanine-glycine (FG) containing.
SPINK, MATTHEW,CHARLES
core  

Permeating the nuclear pore complex [PDF]

Nucleus, 2010
The extensive and multifaceted traffic between nucleus and cytoplasm is handled by a single type of macromolecular assembly called the nuclear pore complex (NPC). While being readily accessible to ions and metabolites, the NPC imposes stringent selectivity on the passage of proteins and RNA, tightly regulating their traffic between the two major ...
Ruti, Kapon, Bracha, Naim, David, Zbaida, Reinat, Nevo, Onie, Tsabari, Ziv, Reich   +5 more
openaire   +2 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

mSphere of Influence: the Dynamic Nature of the Nuclear Envelope during Mitosis of Malaria Parasites

mSphere, 2020
Sabrina Absalon works in the field of cellular and molecular biology of Plasmodium falciparum, the most virulent parasite causing malaria in humans. In this mSphere of Influence article, she reflects on how the paper “3D nuclear architecture reveals ...
Sabrina Absalon
doaj   +1 more source

Asynchronous nuclear division cycles in multinucleated cells [PDF]

, 2006
Synchronous mitosis is common in multinucleated cells. We analyzed a unique asynchronous nuclear division cycle in a multinucleated filamentous fungus, Ashbya gossypii.
Peter Philippsen, Gladfelter, A. S., Amy S. Gladfelter, A. Katrin Hungerbuehler, Hungerbuehler, A. K., Philippsen, P.   +5 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Nuclear Import of Hepatitis B Virus Capsids and Genome

Viruses, 2017
Hepatitis B virus (HBV) is an enveloped pararetrovirus with a DNA genome, which is found in an up to 36 nm-measuring capsid. Replication of the genome occurs via an RNA intermediate, which is synthesized in the nucleus.
Lara Gallucci, Michael Kann
doaj   +1 more source

Repo-Man coordinates chromosomal reorganization with nuclear envelope reassembly during mitotic exit [PDF]

, 2011
This article is available open access under a Creative Commons license (http://creativecommons.org/licenses/by/3.0/). Copyright @ 2011 Elsevier Inc.Repo-Man targets protein phosphatase 1 γ (PP1γ) to chromatin at anaphase onset and regulates chromosome ...
Vagnarelli, Paola, Rappsilber, Juri, Susana Ribeiro, David A. Kelly, Ribeiro, S, Sanchez-Pulido, Luis, Luis Sanchez-Pulido, Ponting, CP, Verheyen, Toon, de Lima Alves, F, Sennels, L, de Lima Alves, Flavia, Ponting, Chris P.; id_orcid, Ponting, Chris P., William C. Earnshaw, Earnshaw, William C., Vagnarelli, P, Sennels, Lau, Kelly, David A., Verheyen, T, Chris P. Ponting, Sanchez-Pulido, L, Ribeiro, Susana, Paola Vagnarelli, Juri Rappsilber, Alves, Flavia de Lima, Kelly, DA, Rappsilber, J, Earnshaw, WC, Lau Sennels, Flavia de Lima Alves, Toon Verheyen   +31 more
core   +1 more source