Results 41 to 50 of about 8,751,128 (381)
In this paper we explore high-throughput Illumina sequencing of nuclear protein-coding, ribosomal, and mitochondrial genes in small, dried insects stored in natural history collections.
K. Kanda+4 more
semanticscholar +1 more source
Nuclear Polyadenylate-Binding Protein [PDF]
Polyadenylate-binding activity can be detected in eluates from sodium dodecyl sulfate gels by a nitrocellulose filter-binding assay. Nuclear extracts from rat liver show a single peak of binding activity at 50 to 55 kilodaltons; cytoplasmic extracts show a single peak at 70 to 80 kilodaltons, corresponding to a 75-kilodalton protein previously ...
Roger D. Kornberg, Alan B. Sachs
openaire +3 more sources
Dual Requirement for Yeast hnRNP Nab2p in mRNA poly(A) Tail Length Control and Nuclear Export [PDF]
Recent studies of mRNA export factors have provided additional evidence for a mechanistic link between mRNA 3′‐end formation and nuclear export. Here, we identify Nab2p as a nuclear poly(A)‐binding protein required for both poly(A) tail length control ...
Anderson, James T.+8 more
core +3 more sources
Hydrogen Sulfide Biology and Its Role in Cancer
Hydrogen sulfide (H2S) is an endogenous biologically active gas produced in mammalian tissues. It plays a very critical role in many pathophysiological processes in the body.
Saadullah Khattak+11 more
doaj +1 more source
Cyclin D1 is a nuclear protein required for cell cycle progression in G1.
A cascade of events is triggered upon the addition of growth factor to quiescent mammalian cells, which ultimately restarts proliferation by inducing the transition from G0/G1 to S-phase. We have studied cyclin D1, a putative G1 cyclin, in normal diploid
ronique Baldin+4 more
semanticscholar +1 more source
Age-related changes in local water and protein content of human eye lenses measured by Raman microspectroscopy [PDF]
The Raman microspectroscopic method was used to determine the local water and protein content in human lenses. In 18 lenses of varying age position-defined water/protein content measurements were carried out along the visual and the equatorial axis.\ud ...
Greve, J.+3 more
core +3 more sources
Nanosecond electric pulses penetrate the nucleus and enhance speckle formation [PDF]
Nanosecond electric pulses generate nanopores in the interior membranes of cells and modulate cellular functions. Here, we used confocal microscopy and flow cytometry to observe Smith antigen antibody (Y12) binding to nuclear speckles, known as small ...
Beebe, S J+9 more
core +1 more source
Proteins Connecting the Nuclear Pore Complex with the Nuclear Interior [PDF]
While much has been learned in recent years about the movement of soluble transport factors across the nuclear pore complex (NPC), comparatively little is known about intranuclear trafficking. We isolated the previously identified Saccharomyces protein Mlp1p (myosin-like protein) by an assay designed to find nuclear envelope (NE) associated proteins ...
Günter Blobel+2 more
openaire +3 more sources
Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. [PDF]
To study the functions of heterogeneous nuclear ribonucleoproteins (hnRNPs), we have characterized nuclear polyadenylated RNA-binding (Nab) proteins from Saccharomyces cerevisiae.
Datar, KV+4 more
core +1 more source
The subnuclear localization of tRNA ligase in yeast [PDF]
Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
core +1 more source