Types of nuclear localization signals and mechanisms of protein import into the nucleus
Cell Communication and Signaling, 2021 Nuclear localization signals (NLS) are generally short peptides that act as a signal fragment that mediates the transport of proteins from the cytoplasm into the nucleus.
Juane Lu +6 more
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Prediction of nuclear proteins using SVM and HMM models
BMC Bioinformatics, 2009 Background The nucleus, a highly organized organelle, plays important role in cellular homeostasis. The nuclear proteins are crucial for chromosomal maintenance/segregation, gene expression, RNA processing/export, and many other processes.
Raghava Gajendra PS, Kumar Manish
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Joint single-cell measurements of nuclear proteins and RNA in vivo. [PDF]
Nat Methods, 2021 Chung H +9 more
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Modeling the transport of nuclear proteins along single skeletal muscle cells. [PDF]
Proc Natl Acad Sci U S A, 2020 Significance Skeletal muscle cells are unique among human cells because they contain hundreds of nuclei distributed over large distances within a shared cytoplasm.
Taylor-Weiner H +6 more
europepmc +2 more sources
Electrophilic PROTACs that degrade nuclear proteins by engaging DCAF16. [PDF]
Nat Chem Biol, 2019 Ligand-dependent protein degradation has emerged as a compelling strategy to pharmacologically control the protein content of cells. So far, however, only a limited number of E3 ligases have been found to support this process.
Zhang X +4 more
europepmc +2 more sources
Single domain antibodies for the knockdown of cytosolic and nuclear proteins. [PDF]
Protein Sci, 2017 Böldicke T.
europepmc +2 more sources
Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins. [PDF]
Sci Rep, 2015 Targeted protein degradation is a powerful tool in determining the function of specific proteins or protein complexes. We fused nanobodies to SPOP, an adaptor protein of the Cullin-RING E3 ubiquitin ligase complex, resulting in rapid ubiquitination and ...
Shin YJ +11 more
europepmc +2 more sources
Degradation of specific nuclear proteins occurs in the cytoplasm in Saccharomyces cerevisiae. [PDF]
Genetics, 2014 Chen L, Madura K.
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Laminopathies: what can humans learn from fruit flies
Cellular & Molecular Biology Letters, 2018 Lamin proteins are type V intermediate filament proteins (IFs) located inside the cell nucleus. They are evolutionarily conserved and have similar domain organization and properties to cytoplasmic IFs. Lamins provide a skeletal network for chromatin, the
Marta Pałka +6 more
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Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myelo\"id cell lines nuclear proteomes [PDF]
, 2012 One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids.
Chevallet, Mireille +5 more
core +4 more sources