Results 261 to 270 of about 624,823 (322)

Application of the Zona-Free Manipulation Technique to Porcine Somatic Nuclear Transfer

Cloning and Stem Cells, 2001
The recent demonstration of a successful zona-free manipulation technique for bovine somatic nuclear transfer (NT) that is both simpler and less labor intensive is of considerable benefit to advance the applications of this technology. Here, we describe that this method is also applicable to porcine somatic NT.
P.J. Booth, Seçil Tan, P. Holm, Henrik Callesen   +3 more
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Evaluation of Lumped Parameter Heat Transfer Techniques for Nuclear Reactor Applications

Nuclear Science and Engineering, 1973
An analytical study was performed to investigate the utility and accuracy of the lumped parameter approach to heat transfer analysis in a nuclear reactor subassembly.
David M. France, T. Ginsberg
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Improvement of a porcine somatic cell nuclear transfer technique by optimizing donor cell and recipient oocyte preparations

Theriogenology, 2003
This study was conducted to improve a porcine somatic cell nuclear transfer (SCNT) technique by optimizing donor cell and recipient oocyte preparations. Adult and fetal fibroblasts, and cumulus and oviduct cells were used as donor cells, and in vivo- and in vitro-matured oocytes were employed as recipient oocytes.
Gab-sang Lee, Sang‐Hwan Hyun, Hye-soo Kim, Dae‐Young Kim, So-Hyun Lee, Jeong-Mook Lim, Eun‐Song Lee, Sung-keun Kang, Byeong Chun Lee, Woo Suk Hwang   +9 more
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134 Generation of NANOS2 knockout goats using CRISPR/Cas9 and somatic cell nuclear transfer techniques

Reproduction, Fertility and Development, 2019
The NANOS2 gene, encoding an RNA binding protein, is known to play a critical role in the development of germline for all organisms studied to date. The male mice with biallelic NANOS2 knockouts (KOs) are sterile due to apoptosis of prospermatogonia shortly after birth but with morphologically intact seminiferous tubules. Thus, the choice of NANOS2 for
Zhiqiang Fan, Misha Regouski, Y. Liu, J. Keim, Iuri Viótti Périssé, Jon M. Oatley, Irina A. Polejaeva   +6 more
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Enhancement of Heat Transfer Performance in Nuclear Fuel Rod Using Nanofluids and Surface Roughness Technique

Volume 8B: Heat Transfer and Thermal Engineering, 2015
The experimental investigations were carried out of a pressurized water nuclear reactor (PWR) with enhanced surface using different concentration (0.5 and 2.0 vol%) of ZnO/DI-water based nanofluids as a coolant. The experimental setup consisted of a flow loop with a nuclear fuel rod section that was heated by electrical current.
Kang Liu, Titan C. Paul, Leo A. Carrilho, Jamil A. Khan   +3 more
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Simplification of Bovine Somatic Cell Nuclear Transfer by Application of a Zona-Free Manipulation Technique

Cloning and Stem Cells, 2001
Contemporary nuclear transfer techniques often require the involvement of skilled personnel and extended periods of micromanipulation. Here, we present details of the development of a nuclear transfer technique for somatic cells that is both simpler and faster than traditional methods.
P.J. Booth, Seçil Tan, R. Reipurth, P. Holm, Henrik Callesen   +4 more
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Development of somatic cell nuclear transfer technique for biomedical and agricultural research (year II) : report

, 2005
There are two objectives of the somatic nuclear transfer studies. The first objective was to evaluate the possibility to produce the inter-species cloned embryos from donor cells of different animals transfer to rabbit, pig or swamp buffalo oocytes. The second objective was to study the donor cell type can significantly affect the in vitro development ...
Mongkol Techakumphu, Pranee Numchaisrika, Ampika Thongpakdee, Rattajuk Rungsiwiwat, Kamtorn Prugsananon, Pramuan Virutamasen   +5 more
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Editing the CYP19 Gene in Goat Embryos Using CRISPR/Cas9 and Somatic Cell Nuclear Transfer Techniques

Cellular Reprogramming
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) system is revolutionizing genome engineering and is expected to bring significant advancements in livestock traits, including the treatment of genetic diseases. This study focuses on CRISPR/Cas9-mediated modifications of the CYP19 gene, which encodes aromatase, an enzyme ...
Ahmad Pirali, Farnoosh Jafarpour, Mehdi Hajian, Seyed Hossein Hosseini Moghaddam, Reza Moradi, Nima Tanhaie-vash, Mohsen Rahimi Andani, Tayebeh Izadi, Hanieh Shiralian-Esfahani, Zahra Safaeinejad, Wilfried A. Kues, Mohammad Hossein Nasr‐Esfahani, Shahin Eghbalsaied   +12 more
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Microinsemination, nuclear transfer, and cytoplasmic transfer: the application of new reproductive engineering techniques to mouse genetics

Mammalian Genome, 2001
Atsuo Ogura, Narumi Ogonuki, Kaoru Takano, Kimiko Inoue   +3 more
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Development of the techniques for nuclear transfer in pigs

Theriogenology, 1999
Nuclear transfer in pigs was developed in the late 1980's. The techniques were based on previous studies in frogs, mice and cattle. Within stage nuclear transfer, pronuclear exchange, was followed by the transfer of nuclei from cleavage stage embryos. While these have resulted in term development, many problems remain.
R S, Prather, T, Tao, Z, Macháty
openaire   +2 more sources