Results 271 to 280 of about 624,823 (322)

Assessment of Gene Transfer: Magnetic Resonance Imaging and Nuclear Medicine Techniques

, 2008
Annette Altmann, Uwe Haberkorn
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Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells

2006
In this chapter, methods are described that permit the enucleation of mouse embryonic stem (ES) cells and the transfer of donor nuclei to these cells before or after enucleation has taken place. The small size and high nucleus-to-cytoplasm volume ratio of ES cells poses a challenge to their enucleation.
Danièle, Pralong, Paul J, Verma
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Comparison of two approaches to nuclear transfer in the bovine: hand-made cloning with modifications and the conventional nuclear transfer technique

Reproduction, Fertility and Development, 2005
The aim of the present study was to compare the in vitro and in vivo developmental competence of hand-made cloning (HMC) embryos with the conventional nuclear transfer (NT) method using five somatic cell lines and in vitro-fertilised (IVF; control) embryos.
Tecirlioglu, R T, Cooney, M A, Lewis, I M, Korfiatis, N A, Hodgson, R, Ruddock, N T, Vajta, Gabor, Downie, S, Trounson, A O, Holland, M K, French, A J   +10 more
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Comparison of gene transcription in cloned bovine embryos produced by different nuclear transfer techniques

Molecular Reproduction and Development, 2001
AbstractThe efficiency of animal production using cloning technology is still relatively low and research to determine a more efficient nuclear transfer procedure is ongoing. One approach which may be informative in assessing the viability of nuclear transfer embryos is the analysis of embryonic gene expression.
R, Daniels, V J, Hall, A J, French, N A, Korfiatis, A O, Trounson   +4 more
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Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer

Biochemical and Biophysical Research Communications, 2006
The low success rate of animal cloning by somatic cell nuclear transfer (SCNT) is believed to be associated with epigenetic errors including abnormal DNA hypermethylation. Recently, we elucidated by using round spermatids that, after nuclear transfer, treatment of zygotes with trichostatin A (TSA), an inhibitor of histone deacetylase, can remarkably ...
Satoshi, Kishigami, Eiji, Mizutani, Hiroshi, Ohta, Takafusa, Hikichi, Nguyen Van, Thuan, Sayaka, Wakayama, Hong-Thuy, Bui, Teruhiko, Wakayama   +7 more
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Comparison of intra- and interspecies nuclear transfer techniques in the production of cloned caprine embryos

Small Ruminant Research, 2011
Abstract Among the ARTs that are applied in goat farming industry, reproductive cloning technology in production of cloned goat embryos are foreseen to facilitate the effort of mass goat production in just a short time frame. There are two possible approaches that can be applied to produce cloned embryos, namely intraspecies SCNT (intraSCNT) and ...
Abdullah, Ramli, Kwong, P.J., Khadijah, W.E.W.   +2 more
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A novel gene-transfer technique mediated by HVJ (Sendai virus), nuclear protein, and liposomes.

Cancer detection and prevention, 1995
There have been many reports of in vivo direct gene transfer methods, but there are problems with these methods. Recently, we developed a novel, nontoxic and efficient in vivo direct gene transfer method mediated by Sendai virus (HVJ) and liposomes. In our system, foreign genes and nuclear proteins were encapsulated into the same liposomes, which were ...
N, Tomita, J, Higaki, T, Ogihara, T, Kondo, Y, Kaneda   +4 more
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Transforming heat transfer with thermal metamaterials and devices

Nature Reviews Materials, 2021
Ying Li, Wei Li, Tiancheng Han
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Transfer Learning Technique Using Similar Phenomena Training for Fuel Digital Twins of Nuclear Systems

13th Nuclear Plant Instrumentation, Control & Human-Machine Interface Technologies (NPIC&HMIT 2023), 2023
James Daniell, Kazuma Kobayashi, Palash Bhowmik, Souvik Chakraborty, Syed Alam   +4 more
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Horizontal gene transfer and adaptive evolution in bacteria

Nature Reviews Microbiology, 2021
Brian J Arnold, William P Hanage
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