Results 51 to 60 of about 713,586 (294)

Establishment of an efficient somatic cell nuclear transfer system for production of transgenic pigs.

Theriogenology, 2012
Handmade cloning (HMC) is now an established procedure used in several species for somatic cell nuclear transfer, but only applied in two related laboratories for pigs.
G. Vajta, H. Callesen
semanticscholar   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Proximal Molecular Probe Transfer (PROMPT), a new approach for identifying sites of protein/nucleic acid interaction in cells by correlated light and electron microscopy

Scientific Reports, 2023
The binding and interaction of proteins with nucleic acids such as DNA and RNA constitutes a fundamental biochemical and biophysical process in all living organisms.
Guillaume A. Castillon, Sebastien Phan, Junru Hu, Daniela Boassa, Stephen R. Adams, Mark H. Ellisman   +5 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Improved 19F{1H} Saturation Transfer Difference Experiments for Sensitive Detection to Fluorinated Compound Bound to Proteins

Magnetochemistry, 2018
The 19F{1H} saturation transfer difference (STD) method was improved for sensitive 19F detection using a human serum albumin-diflunisal complex. Because NMR (nuclear magnetic resonance) experiments with 19F detection are feasible for the selective ...
Kazuo Furihata, Mitsuru Tashiro
doaj   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

Mitochondrial Donation: The UK Experience

Fertility & Reproduction, 2023
Mutations in mitochondrial DNA (mtDNA) are inherited exclusively from our mothers and can cause a broad range of debilitating and fatal diseases. Reproductive technologies designed to uncouple the inheritance of the mitochondrial genome from the nuclear ...
Mary Herbert
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Reconstruction of mammalian oocytes by germinal vesicle transfer: A systematic review

International Journal of Reproductive BioMedicine, 2017
Nuclear transfer procedures have been recently applied for clinical and research targets as a novel assisted reproductive technique and were used for increasing the oocyte activity during its growth and maturation.
Sara Darbandi, Mahsa Darbandi, Hamid Reza Khorram Khorshid, Abolfaz Shirazi, Mohammad Reza Sadeghi, Ashok Agarwal, Safaa Al-Hasani, Mohammad Mehdi Naderi, Ahmet Ayaz, Mohammad Mehdi Akhondi   +9 more
doaj  

Coherent gamma photon generation in a Bose–Einstein condensate of 135mCs

Physics Letters B, 2018
We have identified a mechanism of collective nuclear de-excitation in a Bose–Einstein condensate of 135Cs atoms in their isomeric state, 135mCs, suitable for the generation of coherent gamma photons.
Luca Marmugi, Philip M. Walker, Ferruccio Renzoni   +2 more
doaj   +1 more source