Genetic Code Expansion and Bio-Orthogonal Labeling Reveal Intact HIV-1 Capsids inside the Nucleus
mBio, 2022 Microscopy is one of the few techniques that can directly observe the HIV-1 capsid as it traverses the cell. However, an extrinsic or intrinsic label is needed to facilitate detection and this can perturb capsid behavior. Now, S. Schifferdecker, V. Zila,
Leo C. James
doaj +1 more source
Molecular Dynamics Analysis of Biomolecular Systems Including Nucleic Acids [PDF]
arXiv, 2021 Along with recent progress in structural biology and genome biology, structural dynamics of molecular systems including nucleic acids has attracted attention in the context of gene regulation. Structure-function relationship is an important topic, where physicochemical properties of nucleotides are important as well as those of amino acids in proteins.
arxiv
Nanosecond chain dynamics of single-stranded nucleic acids [PDF]
arXiv, 2023 The conformational dynamics of single-stranded nucleic acids are fundamental for nucleic acid folding and function. However, their elementary chain dynamics have been difficult to resolve experimentally. Here we employ a combination of single-molecule F\"orster resonance energy transfer, nanosecond fluorescence correlation spectroscopy, fluorescence ...
arxiv
Paradigms for computational nucleic acid design [PDF]
, 2004 The design of DNA and RNA sequences is critical for many endeavors, from DNA nanotechnology, to PCRâbased applications, to DNA hybridization arrays.
Dirks, Robert M. +3 more
core +5 more sources
Identification and differentiation among chicken's, duck's, quail's, rabbit's and turkey's meat using PCR-RFLP technique [PDF]
Biotechnology in Animal Husbandry, 2015 PCR-RFLP technique was developed for identification and differentiation among chicken's, duck's, quail's, rabbit's and turkey's meat. DNA from small amount of muscles (0.05 g) was extracted and a region of mitochondrial DNA (cytochrome-b gene) in chicken,
Abdel-Rahman S.M. +2 more
doaj
Mechanisms of HIV-1 Nucleocapsid Protein Inhibition by Lysyl-Peptidyl-Anthraquinone Conjugates [PDF]
, 2016 The Nucleocapsid protein NCp7 (NC) is a nucleic acid chaperone responsible for essential steps of the HIV-1 life cycle and an attractive candidate for drug development.
Alice Sosic +12 more
core +2 more sources
Application of magnetic nanoparticles in nucleic acid detection
Journal of Nanobiotechnology, 2020 Nucleic acid is the main material for storing, copying, and transmitting genetic information. Gene sequencing is of great significance in DNA damage research, gene therapy, mutation analysis, bacterial infection, drug development, and clinical diagnosis.
Congli Tang +12 more
semanticscholar +1 more source
The Nucleic Acid Database: A Resource for Nucleic Acid Science [PDF]
Acta Crystallographica Section D Biological Crystallography, 1998 The Nucleic Acid Database (NDB) distributes information about nucleic acid-containing structures. Here the information content of the database as well as the query capabilities are described. A summary of how the technology developed by this project has been used to develop other macromolecular databases is given.
John D. Westbrook +2 more
openaire +3 more sources
On-Chip DNA Methylation Analysis Using Osmium Complexation
Journal of Nucleic Acids, 2011 The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject.
Kaori Sugizaki +2 more
doaj +1 more source
Developing methods to study conformational changes in RNA crystals using a photocaged ligand
Frontiers in Molecular Biosciences, 2022 Crystallographic observation of structural changes in real time requires that those changes be uniform both spatially and temporally. A primary challenge with time-resolved ligand-mixing diffraction experiments is asynchrony caused by variable factors ...
Hyun Kyung Lee +8 more
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