Mycoplasma pneumoniae: Current knowledge on nucleic acid amplification techniques and serological diagnostics [PDF]
Frontiers in Microbiology, 2016 Mycoplasma pneumoniae (M. pneumoniae) belongs to the class Mollicutes and has been recognized as a common cause of respiratory tract infections (RTIs), including community-acquired pneumonia (CAP), that occur worldwide and in all age groups. In addition,
Katherine eLoens, Margareta eIeven
doaj +7 more sources
Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR [PDF]
Practical Laboratory MedicineIsothermal nucleic acid amplification methods have gained prominence as a valuable innovation in molecular diagnostics. These methods function at a constant temperature without the requirement of thermal cycler apparatus, simplifying the amplification ...
Alazar Amare Amdiyee +1 more
doaj +3 more sources
Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis. [PDF]
Biochemistry (Mosc), 2020 Recently, there has been a rapid progress in the development of techniques for isothermal amplification of nucleic acids as an alternative to polymerase chain reaction (PCR). The advantage of these methods is that the nucleic acids amplification can be carried out at constant temperature, unlike PCR, which requires cyclic temperature changes. Moreover,
Bodulev OL, Sakharov IY.
europepmc +5 more sources
The cost effectiveness of Nucleic Acid Amplification Techniques for the diagnosis of tuberculosis [PDF]
Respiratory Medicine, 2011 There is wide variation in the techniques deployed to diagnose tuberculosis in the UK, with little agreement on which tools or strategies are cost effective. This analysis therefore comprehensively evaluated the cost effectiveness of currently available diagnostic strategies for routine diagnosis of TB in the NHS.The analysis compared strategies ...
Ralph Hughes +3 more
openalex +3 more sources
Detection of Nucleic Acids with a Novel Stem-Loop Primer Rolling Circle Amplification Technique [PDF]
BioTechniques, 2018 This paper presents a new rolling circle amplification (RCA) technique using stem-loop primers (SLP). The technique enables detection of target DNA by either linear or exponential amplification (SLP-lRCA and SLP-eRCA) in both liquid and solid phases. For solid-phase detection, SLP-eRCA detects nucleic acids in four steps: (1) covalently immobilize an ...
Beibei Zhang +4 more
openalex +4 more sources
Nucleic acid amplification-based techniques for pathogen detection and identification
Infection, Genetics and Evolution, 2005 Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and ...
Paul Monis, Steven Giglio
openalex +4 more sources
Molecular and Cellular Probes, 1995 A set of primers (BTV-pr1/2) were selected that hybridized to the VP3 gene of the major North American serotypes of bluetongue virus (BTV). Polymerase chain reaction (PCR) testing yielded positive results from specimens of major North American BTV isolates (serotypes 10, 11, 13 and 17) propagated in Vero cells.
Martha J. Harding +2 more
openalex +3 more sources
Review on Application of Nucleic Acid Amplification Techniques in Pharmaceutical Products Analysis [PDF]
Journal of Applied Pharmaceutical Science, 2015 The aim of this study was to describe application of molecular diagnostic tests based on nucleic acid amplification technologies (NAATs) in pharmaceutical product analysis. NAAT have become widely established in clinical microbiology laboratories in recent years, as well as in quality control (QC) laboratories for food testing, and lately introduced to
Mohammad Saleh Al-Aboody
openalex +2 more sources
Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection [PDF]
Talanta, 2023 Nucleic acid amplification techniques have always been one of the hot spots of research, especially in the outbreak of COVID-19. From the initial polymerase chain reaction (PCR) to the current popular isothermal amplification, each new amplification techniques provides new ideas and methods for nucleic acid detection.
Hao Jiang +4 more
openalex +3 more sources
Nucleic acid amplification techniques for rapid diagnosis of nontuberculous mycobacteria: A protocol of systematic review and meta-analysis. [PDF]
PLoS One, 2021 Yu G, Shen Y, Xu X, Lin L.
europepmc +3 more sources