Mycoplasma pneumoniae: Current knowledge on nucleic acid amplification techniques and serological diagnostics [PDF]
Frontiers in Microbiology, 2016 Mycoplasma pneumoniae (M. pneumoniae) belongs to the class Mollicutes and has been recognized as a common cause of respiratory tract infections (RTIs), including community-acquired pneumonia (CAP), that occur worldwide and in all age groups. In addition,
Katherine eLoens, Margareta eIeven
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Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis. [PDF]
Biochemistry (Mosc), 2020 Recently, there has been a rapid progress in the development of techniques for isothermal amplification of nucleic acids as an alternative to polymerase chain reaction (PCR). The advantage of these methods is that the nucleic acids amplification can be carried out at constant temperature, unlike PCR, which requires cyclic temperature changes. Moreover,
Bodulev OL, Sakharov IY.
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Nucleic acid amplification techniques for rapid diagnosis of nontuberculous mycobacteria: A protocol of systematic review and meta-analysis. [PDF]
PLoS One, 2021 Yu G, Shen Y, Xu X, Lin L.
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Standardization of SARS-CoV-2 Nucleic Acid Amplification Techniques by Calibration and Quantification to the First WHO International Standard for SARS-CoV-2 RNA. [PDF]
Int J Microbiol, 2023 Voermans JJC +8 more
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Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses. [PDF]
Anal Chim Acta, 2022 Islam MM, Koirala D.
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A direct multiplex isothermal amplification-reverse dot blot hybridization system for β-thalassemia diagnosis [PDF]
Annals of HematologyMolecular diagnostic methods largely rely on expensive equipment and complex operations, which makes it difficult to achieve rapid and low-cost detection.
Chao Ye +8 more
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Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection
Chemosensors, 2022 Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive.
Thi Ngoc Diep Trinh, Nae Yoon Lee
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A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions
Sensors and Actuators Reports, 2021 Isothermal nucleic acid amplification techniques are used to detect a variety of molecules such as RNAs, DNAs and proteins. When compared to the gold standard polymerase chain reaction, isothermal methods are rapid and simple but often have lower ...
Burcu Özay, Stephanie E McCalla
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Molecular Mechanisms Underlying CRISPR/Cas-Based Assays for Nucleic Acid Detection
Current Issues in Molecular Biology, 2023 Applied to investigate specific sequences, nucleic acid detection assays can help identify novel bacterial and viral infections. Most up-to-date systems combine isothermal amplification with Cas-mediated detection.
Denis N. Antropov, Grigory A. Stepanov
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Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review
Biosensors, 2023 Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to ...
Soo Min Lee +4 more
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