Results 111 to 120 of about 186,936 (344)

Cas12a Cis-cleavage mediated lateral flow assay enables multiplex and ultra-specific nucleic acid detection

Nature Communications
CRISPR technology holds significant promise for advancing nucleic acid assays. However, current CRISPR diagnostic techniques, reliant on indiscriminate trans-cleavage mechanisms, face challenges in developing multiplex detection formats.
Mei Lin, Zhiqiang Qiu, Mengen Hao, Weiwei Qi, Ting Zhang, Yuting Shen, Hongrui Xiao, Chaoyue Liang, Longxu Xie, Yongzhong Jiang, Meng Cheng, Tian Tian, Xiaoming Zhou   +12 more
doaj   +1 more source

A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan. [PDF]

Biologicals, 2022
Lin PL, Wu MS, Wu PC, Chen HM, Peng YH, Hsu JC, Wang DY.   +6 more
europepmc   +1 more source

Development of a Photothermal Bead-Based Nucleic Acid Amplification Test (Pbbnaat) Technique for a High-Performance Loop-Mediated Isothermal Amplification (Lamp)–Based Point-of-Care Test (Poct)

, 2022
Min‐Hsien Wu, Chih-Yu Chen, Hung Wei Yang, Ping-Han Hsieh, Chia‐Hsun Hsieh   +4 more
openalex   +1 more source

Detection of cryptosporidium oocysts in water and environmental concentrates [PDF]

, 1997
Whilst current methods for the isolation and enumeration of Cryptosporidium spp. oocysts in water have provided some insight into their occurrence and significance, they are regarded as being inefficient, variable and time-consuming, with much of the ...
Smith, H.V.
core  

cDNA-RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG. [PDF]

, 2001
Identifying genes that are differentially expressed by Mycobacterium bovis BCG after phagocytosis by macrophages will facilitate the understanding of the molecular mechanisms of host cell-intracellular pathogen interactions. To identify such genes a cDNA-
Centre Gunnels Wood, Irene M. Monahan, Joseph A. Mangan, Martin J. Everett, Ming-shi Li, Philip D. Butcher, Simon J. Waddell, Steve L. Martin   +7 more
core   +3 more sources

From RNA to DNA: How Cargo Identity Reprograms Lipid Nanoparticle Architecture and Function

Advanced Healthcare Materials, EarlyView.
The evolution of lipid nanoparticles (LNPs) spans from RNA‐LNPs, used in mRNA vaccines, to DNA‐LNPs, ideal for gene therapies. Emerging bionano architectures, decorated with DNA and plasma proteins, pave the way for advanced DNA‐based therapies that are more stable, targeted, and customizable.
Erica Quagliarini, Daniela Pozzi, Giulio Caracciolo   +2 more
wiley   +1 more source

Rapid Manufacturing of Glass‐Based Digital Nucleic Acid Amplification Chips by Ultrafast Bessel Pulses

Small Science
Advanced nucleic acid amplification techniques require separating test samples into numerous units (digitalization), which enables higher sensitivity and accuracy than traditional methods.
Jiawei Zhang, Kotaro Obata, Kazunari Ozasa, Takanori Uzawa, Yoshihiro Ito, Koji Sugioka   +5 more
doaj   +1 more source

Advancing transcriptome platforms [PDF]

, 2011
During the last decade of years, remarkable technological innovations have emerged that allow the direct or indirect determination of the transcriptome at unprecedented scale and speed.
Shuobo Shi
core   +2 more sources

Bacteria‐Responsive Nanostructured Drug Delivery Systems for Targeted Antimicrobial Therapy

Advanced Materials, EarlyView.
Bacteria‐responsive nanocarriers are designed to release antimicrobials only in the presence of infection‐specific cues. This selective activation ensures drug release precisely at the site of infection, avoiding premature or indiscriminate release, and enhancing efficacy.
Guillermo Landa, Gracia Mendoza, Silvia Irusta, Manuel Arruebo   +3 more
wiley   +1 more source

Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives

Advanced Materials, EarlyView.
Kelvin probe force microscopy (KPFM) enables the nanoscale mapping of electrostatic surface potentials. While widely applied in materials science, its use in biological systems remains emerging. This review presents recent advances in KPFM applied to biological samples and provides a critical perspective on current limitations and future directions for
Ehsan Rahimi, Mario Palacios‐Corella, Arjan Mol, Salvador Pané, Josep Puigmartí‐Luis   +4 more
wiley   +1 more source