Results 11 to 20 of about 186,936 (344)
The cost effectiveness of Nucleic Acid Amplification Techniques for the diagnosis of tuberculosis [PDF]
Respiratory Medicine, 2011 There is wide variation in the techniques deployed to diagnose tuberculosis in the UK, with little agreement on which tools or strategies are cost effective. This analysis therefore comprehensively evaluated the cost effectiveness of currently available diagnostic strategies for routine diagnosis of TB in the NHS.The analysis compared strategies ...
Ralph Hughes +3 more
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Detection of Nucleic Acids with a Novel Stem-Loop Primer Rolling Circle Amplification Technique [PDF]
BioTechniques, 2018 This paper presents a new rolling circle amplification (RCA) technique using stem-loop primers (SLP). The technique enables detection of target DNA by either linear or exponential amplification (SLP-lRCA and SLP-eRCA) in both liquid and solid phases. For solid-phase detection, SLP-eRCA detects nucleic acids in four steps: (1) covalently immobilize an ...
Beibei Zhang +4 more
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Nucleic acid amplification-based techniques for pathogen detection and identification
Infection, Genetics and Evolution, 2005 Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and ...
Paul Monis, Steven Giglio
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Molecular and Cellular Probes, 1995 A set of primers (BTV-pr1/2) were selected that hybridized to the VP3 gene of the major North American serotypes of bluetongue virus (BTV). Polymerase chain reaction (PCR) testing yielded positive results from specimens of major North American BTV isolates (serotypes 10, 11, 13 and 17) propagated in Vero cells.
Martha J. Harding +2 more
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Review on Application of Nucleic Acid Amplification Techniques in Pharmaceutical Products Analysis [PDF]
Journal of Applied Pharmaceutical Science, 2015 The aim of this study was to describe application of molecular diagnostic tests based on nucleic acid amplification technologies (NAATs) in pharmaceutical product analysis. NAAT have become widely established in clinical microbiology laboratories in recent years, as well as in quality control (QC) laboratories for food testing, and lately introduced to
Mohammad Saleh Al-Aboody
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Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection [PDF]
Talanta, 2023 Nucleic acid amplification techniques have always been one of the hot spots of research, especially in the outbreak of COVID-19. From the initial polymerase chain reaction (PCR) to the current popular isothermal amplification, each new amplification techniques provides new ideas and methods for nucleic acid detection.
Hao Jiang +4 more
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Chemosensors, 2022 The determination of nucleic acids has become an analytical diagnostic method with many applications in fields such as biomedical sciences, environmental monitoring, forensic identification, and food safety.
Claudia Patiti +7 more
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A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain
Synthetic and Systems Biotechnology, 2023 Nucleic acid detection plays a key role in diverse diagnosis and disease control. Currently available nucleic acid detection techniques are challenged by trade-offs among speed, simplicity, precision and cost.
Yuting Shuai +10 more
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Application of the OSNA Technique (One-Step Nucleic Acid Amplification Test) in Breast Cancer [PDF]
International Journal of Molecular SciencesBreast cancer is one of the most common cancers diagnosed in both countries with high and low levels of socio-academic development. Routine, regular screening tests being introduced in an increasing number of countries make it possible to detect breast cancer at an early stage of development, as a result of which the trend in the incidence of ...
Grzegorz Sychowski +2 more
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Simple system for isothermal DNA amplification coupled to lateral flow detection. [PDF]
PLoS ONE, 2013 Infectious disease diagnosis in point-of-care settings can be greatly improved through integrated, automated nucleic acid testing devices. We have developed an early prototype for a low-cost system which executes isothermal DNA amplification coupled to ...
Kristina Roskos +6 more
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